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Cited by 112Neurotransmitter Receptor Influence on BehaviorNeuroscience and Neuropharmacology ResearchDeep Brain Stimulation and Movement Disorders

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Effects of dopamine depletion on the morphology of medium spiny neurons in the shell and core of the rat nucleus accumbens

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Meredith, GE, Ypma, P, Zahm, DS·1995·The Journal of Neuroscience
DOI 10.1523/jneurosci.15-05-03808.1995PMC PMC6578201
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Abstract

Nucleus accumbens receives a dense dopaminergic innervation which is important in regulating motivated states of behavior such as goal-directed actions, stimulus-reward associations and reinforcement of addictive substances. The shell and core territories of this nucleus each receive functionally and morphologically distinct dopaminergic inputs and lesions of the ascending pathways totally deprive the core but not the shell of dopaminergic fibers. Medium spiny neurons are the principal targets of dopaminergic terminals. The present study explored whether the loss of dopamine inputs can affect these neurons and whether cells in the shell and core would be equally susceptible to such a loss. Intracellular injection in fixed slices and neuronal reconstruction were used to analyze the dendritic trees of 62 neurons in the shell and core of animals that received a unilateral, chronic 6-hydroxydopamine lesion of the medial forebrain bundle. In the dopamine-depleted core, dendrites are significantly shorter (16% decrease) than in the intact core and in both the dopamine-depleted core and lateral shell, dendrites are less spiny than in respective control regions. Dopamine loss in the medial shell is associated with significantly more tortuous dendrites that are lower in spine density. However, the number of spines is not reduced which may mean that the increase recorded for segment length, although insignificant in tests, could be responsible for the change in spine density. These data suggest that the loss of dopamine can affect accumbal neuronal morphology and, moreover, can affect neuronal structures differentially in the shell and core.

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Registry

Crossref · 10.1523/jneurosci.15-05-03808.1995

Cites

66 of 66 references verified0 citation errors · 0 not checkable

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Figures

Figure 1 A, Photomicrograph of tyrosine hydroxylase immunoreactivity in a transverse section through the brain of a rat that received a unilateral injection of 6-OHDA in the medial forebrain bundle 21 days earlier. Note the near complete absence of tyrosine hydroxylase immunoreactivity on one side. B, Higher magnification of a portion of the medial accumbal shell in the DA-depleted hemisphere. Note that remaining tyrosine hydroxylase-immunoreactive fibers and varicosities are visible (open arrowhead). The star (B) marks the location of the anterior commissure. Scale bars: A,500μm; B, 100μm.
Figure 1 A, Photomicrograph of tyrosine hydroxylase immunoreactivity in a transverse section through the brain of a rat that received a unilateral injection of 6-OHDA in the medial forebrain bundle 21 days earlier. Note the near complete absence of tyrosine hydroxylase immunoreactivity on one side. B, Higher magnification of a portion of the medial accumbal shell in the DA-depleted hemisphere. Note that remaining tyrosine hydroxylase-immunoreactive fibers and varicosities are visible (open arrowhead). The star (B) marks the location of the anterior commissure. Scale bars: A,500μm; B, 100μm.CC-BY-NC-SA · From the PDF
Image 2
CC-BY-NC-SA · From the PDF
Figure 2 Diagrams showing the position of neurons filled with LY (solid circles) in sections through mid- to caudal levels of the nucleus accumbens. Core, lateral shell (LS) and medial shell (MS) are marked and A shows cells injeċted in the control side; B shows neurons filled in the DA-depleted hemisphere.
Figure 2 Diagrams showing the position of neurons filled with LY (solid circles) in sections through mid- to caudal levels of the nucleus accumbens. Core, lateral shell (LS) and medial shell (MS) are marked and A shows cells injeċted in the control side; B shows neurons filled in the DA-depleted hemisphere.CC-BY-NC-SA · From the PDF
Figure 3 Photomicrographs of two LY-immunoreacted core neurons (A, C) found in the first section of a slice and the full reconstructions of those neurons (B, D). A and B are from the intact hemisphere and C and D from the DA-depleted core. Arrows point to the proximal aspiny segments of these cells. Note that spines begin at or beyond the first branch point. A star marks a filled cell with filamentous dendrites. Scale bars, 25 μm.
Figure 3 Photomicrographs of two LY-immunoreacted core neurons (A, C) found in the first section of a slice and the full reconstructions of those neurons (B, D). A and B are from the intact hemisphere and C and D from the DA-depleted core. Arrows point to the proximal aspiny segments of these cells. Note that spines begin at or beyond the first branch point. A star marks a filled cell with filamentous dendrites. Scale bars, 25 μm.CC-BY-NC-SA · From the PDF
Image 5
CC-BY-NC-SA · From the PDF
Figure 5 High magnification photomicrographs of LY-immunoreacted dendritic segments in the intact (A) and DA-depleted (B) medial shell. Arrowheads point out the different shapes and sizes of spines. Segments pictured in C and D are derived from neurons in the intact and DAdepleted lateral shell, respectively. Arrowheads in C point out the asymmetry in spine distribution along the segment, and arrowhead in D points to cone-like termination of the dendrite. Scale bar in A is valid for B and equals 10 μm, and scale bar in C is valid for D and equals 10 μm.
Figure 5 High magnification photomicrographs of LY-immunoreacted dendritic segments in the intact (A) and DA-depleted (B) medial shell. Arrowheads point out the different shapes and sizes of spines. Segments pictured in C and D are derived from neurons in the intact and DAdepleted lateral shell, respectively. Arrowheads in C point out the asymmetry in spine distribution along the segment, and arrowhead in D points to cone-like termination of the dendrite. Scale bar in A is valid for B and equals 10 μm, and scale bar in C is valid for D and equals 10 μm.CC-BY-NC-SA · From the PDF
ed d ed od (d s ad e ( od -nd nd - tnd rined parts of some dendritic segments. Note that the tortuous segments in (A) in the second or third branch order.
ed d ed od (d s ad e ( od -nd nd - tnd rined parts of some dendritic segments. Note that the tortuous segments in (A) in the second or third branch order.CC-BY-NC-SA · From the PDF
Image 8
CC-BY-NC-SA · From the PDF

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Research works printed but not linked: 1

  1. Groenewegen HJ, Berendse HW, Meredith GE, Haber SN, Voorn P, Wolters JG, Lohman AHM (1991) Functional anatomy of the ven- tral, limbic system innervated striatum. In: The mesolimbic dopamine system: from motivation to action (Willner P Scheel-Kruger J, eds), pp 19-59. Chichester: Wiley.

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Metadata

Authors
Meredith, G., Ypma, P., & Zahm, D.
Affiliations
Vrije Universiteit Amsterdam (NL)
Venue
The Journal of Neuroscience
Publisher
Society for Neuroscience
Published
1995-05-01
Volume
15
Issue
5
Pages
3808-3820
ISSN
  • 0270-6474 (ISSN-L)
  • 1529-2401
DOI
10.1523/jneurosci.15-05-03808.1995
PMC
PMC6578201
OpenAlex
W1589481227
Cited by
112
References
68
Citations by year
2025: 1 · 2024: 3 · 2023: 2 · 2022: 3 · 2021: 2 · 2020: 2 · 2019: 2 · 2018: 2 · 2017: 3 · 2016: 4 · 2015: 2 · 2014: 3 · 2013: 3 · 2012: 5
FWCI
1.87
Type
journal article
Language
en
Open access
Licence
Locations
  • Journal of Neuroscience (publishedVersion, cc-by-nc-sa)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • U.S. Public Health Service
  • National Institute of Neurological Disorders and Stroke (NS23805, R01NS023805)
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurotransmitter Receptor Influence on Behavior
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Health Sciences › Medicine › Clinical Neurology › Deep Brain Stimulation and Movement Disorders
Keywords
dopamine depletion, medium spiny neurons, nucleus accumbens, dendritic morphology, dendritic spines, dendritic spine density, 6-hydroxydopamine lesion, medial forebrain bundle, dopaminergic innervation, neuron reconstruction
MeSH terms
Animals, Dendrites, Dopamine, Male, Nerve Endings, Neurons, Nucleus Accumbens, Reference Values, Tyrosine 3-Monooxygenase, Prosencephalon, Oxidopamine, Rats, Wistar, Rats

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