Cited by 101Cellular transport and secretionNeuroscience and Neuropharmacology ResearchRNA Research and Splicing

Developmentally Regulated Switch in Alternatively Spliced SNAP-25 Isoforms Alters Facilitation of Synaptic Transmission

Open access
Bark, Christina, Bellinger, Frederick P., Kaushal, Ashutosh, Mathews, James R., Partridge, L. Donald, Wilson, Michael C.·2004·The Journal of Neuroscience

Abstract

Although the basic molecular components that promote regulated neurotransmitter release are well established, the contribution of these proteins as regulators of the plasticity of neurotransmission and refinement of synaptic connectivity during development is elaborated less fully. For example, during the period of synaptic growth and maturation in brain, the expression of synaptosomal protein 25 kDa (SNAP-25), a neuronal t-SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) essential for action potential-dependent neuroexocytosis, is altered through alternative splicing of pre-mRNA transcripts. We addressed the role of the two splice-variant isoforms of SNAP-25 with a targeted mouse mutation that impairs the shift from SNAP-25a to SNAP-25b. Most of these mutant mice die between 3 and 5 weeks of age, which coincides with the time when SNAP-25b expression normally reaches mature levels in brain and synapse formation is essentially completed. The altered expression of these SNAP-25 isoforms influences short-term synaptic function by affecting facilitation but not the initial probability of release. This suggests that mechanisms controlling alternative splicing between SNAP-25 isoforms contribute to a molecular switch important for survival that helps to guide the transition from immature to mature synaptic connections, as well as synapse regrowth and remodeling after neural injury.

Integrity signals

Registry

Crossref · 10.1523/jneurosci.1940-04.2004

Cites

54 of 54 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

We couldn’t retrieve this paper’s full text, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • SNAP25 isoforms show differences in the kinetics of synaptic vesicle release.

    “that show differences in the kinetics of synaptic vesicle release138,139.”

    From The neurogenetics of alternative splicing (2016) · cites this paper as [139]

  • The SNAP25 gene uses mutually exclusive exons 5a and 5b to produce two alternative isoforms.

    “the gene for the SNARE protein SNAP25 uses a pair of mutually exclusive exons (5a and 5b) to produce two alternative isoforms”

    From The neurogenetics of alternative splicing (2016) · cites this paper as [139]

Cites

Flagged first, then by citations

1 of the 55 references couldn’t be matched to a known paper.

Cited by

Sorted by citations

Authors
Bark, C., Bellinger, F. P., Kaushal, A., Mathews, J. R., Partridge, L. D., & Wilson, M. C.
Affiliations
  • Karolinska University Hospital (SE)
  • University of New Mexico (US)
Corresponding author
Wilson, Michael C.
Venue
The Journal of Neuroscience
Published
2004-10-06
Volume
24
Issue
40
Pages
8796-8805
ISSN
  • 0270-6474 (ISSN-L)
  • 1529-2401
OpenAlex
W1967517418
Cited by
101
References
55
Citations by year
2025: 4 · 2024: 1 · 2023: 2 · 2022: 1 · 2021: 3 · 2020: 3 · 2019: 2 · 2018: 6 · 2017: 3 · 2016: 6 · 2015: 2 · 2014: 7 · 2013: 6 · 2012: 8
FWCI
2.80
Type
journal article
Language
en
Open access
Locations
  • Journal of Neuroscience (publishedVersion, cc-by-nc-sa)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Karolinska Institutet
  • National Institutes of Health
  • National Institute of Mental Health (MH 4-8989, R01MH048989)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
Keywords
SNAP-25, alternative splicing, synaptic facilitation, neurotransmitter release, synapse maturation, t-SNARE, short-term plasticity, synaptogenesis, mouse mutants
MeSH terms
Animals, Hippocampus, Membrane Proteins, Mice, Mutant Strains, Nerve Tissue Proteins, Synaptic Transmission, Neuronal Plasticity, Neurons, Phenotype, Alternative Splicing, Gene Targeting, Patch-Clamp Techniques, Gene Expression Regulation, Developmental, Integrases, Protein Isoforms, Synaptosomal-Associated Protein 25, Mice

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review