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Cited by 1,183Neuroscience and Neuropharmacology ResearchCellular transport and secretionNeural dynamics and brain function

Heterogeneity of release probability, facilitation, and depletion at central synapses

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Dobrunz, Lynn E., Stevens, Charles F.·1997·Neuron
DOI 10.1016/s0896-6273(00)80338-4
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Abstract

Previous studies of short-term plasticity in central nervous systems synapses have largely focused on average synaptic properties. In this study, we use recordings from putative single synaptic release sites in hippocampal slices to show that significant heterogeneity exists in facilitation and depletion among synapses. In particular, the amount of paired-pulse facilitation is inversely related to the initial release probability of the synapse. We also examined depletion at individual synapses using high frequency stimulation, and estimated the size of the readily releasable vesicle pool, which averaged 5.0 +/- 3.0 quanta (n = 13 synapses). In addition, these experiments demonstrate that the release probability at a synapse is directly correlated with the size of its readily releasable vesicle pool.

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  • The number of vesicles adjacent to the presynaptic cleft is proportional to those primed for synaptic transmitter release.

    “The area of the postsynaptic density is proportional to the number of postsynaptic receptors (Nusser et al., 1998), while the number of vesicles adjacent to the presynaptic cleft is proportional to those primed for synaptic transmitter release (Dobrunz and Stevens, 1997).”

    From The influence of phospho-tau on dendritic spines of cortical pyramidal neurons in patients with Alzheimer’s disease (2013) · cites this paper as Dobrunz and Stevens, 1997

    The passage on the physical interpretation of the readily releasable pool (indexed as Abstract; no page given) says that this pool may correspond roughly to morphologically docked vesicles. It explicitly says that identifying the two pools is “far from established” and calls for a demonstration that their sizes vary correspondingly. Thus, it supports a possible relationship, not the claimed proportionality as a settled fact. (LLM, conf=0.88)

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Cites

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Research works printed but not linked: 25

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Cited by

Sorted by citations

Authors
Dobrunz, L. E., & Stevens, C. F.
Affiliations
Salk Institute for Biological Studies (US)
Corresponding author
Stevens, Charles F.
ORCID
Venue
Neuron
Publisher
Elsevier BV
Published
1997-06
Volume
18
Issue
6
Pages
995-1008
ISSN
0896-6273 (ISSN-L)
DOI
10.1016/s0896-6273(00)80338-4
OpenAlex
W2019016101
Cited by
1,183
References
3
Citations by year
2026: 21 · 2025: 21 · 2024: 37 · 2023: 27 · 2022: 23 · 2021: 57 · 2020: 32 · 2019: 54 · 2018: 39 · 2017: 38 · 2016: 33 · 2015: 45 · 2014: 46 · 2013: 42 · 2012: 57
FWCI
16.10
Type
journal article
Language
en
Open access
Licence
Locations
  • Neuron (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
  • Life Sciences › Neuroscience › Cognitive Neuroscience › Neural dynamics and brain function
Keywords
paired-pulse facilitation, short-term plasticity, hippocampus, central synapses, synaptic heterogeneity
MeSH terms
Animals, Brain Stem, Electric Stimulation, Evoked Potentials, Hippocampus, Neural Inhibition, Neuronal Plasticity, Synapses, Synaptic Vesicles, Time Factors, Receptors, Metabotropic Glutamate, Patch-Clamp Techniques, Rats, In Vitro Techniques

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