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Endothelial-Specific Knockdown of Interleukin-1 (IL-1) Type 1 Receptor Differentially Alters CNS Responses to IL-1 Depending on Its Route of Administration
Open accessAbstract
Interleukin-1 (IL-1) has been implicated as a critical mediator of neuroimmune communication. In the brain, the functional receptor for IL-1, type 1 IL-1 receptor (IL-1R1), is localized primarily to the endothelial cells. In this study, we created an endothelial-specific IL-1R1 knockdown model to test the role of endothelial IL-1R1 in mediating the effects of IL-1. Neuronal activation in the hypothalamus was measured by c-fos expression in the paraventricular nucleus and the ventromedial preoptic area. In addition, two specific sickness symptoms, febrile response and reduction of locomotor activity, were studied. Intracerebroventricular injection of IL-1 induced leukocyte infiltration into the CNS, activation of hypothalamic neurons, fever, and reduced locomotor activity in normal mice. Endothelial-specific knockdown of IL-1R1 abrogated all these responses. Intraperitoneal injection of IL-1 also induced neuronal activation in the hypothalamus, fever, and reduced locomotor activity, without inducing leukocyte infiltration into the brain. Endothelial-specific knockdown of IL-1R1 suppressed intraperitoneal IL-1-induced fever, but not the induction of c-fos in hypothalamus. When IL-1 was given intravenously, endothelial knockdown of IL-1R1 abolished intravenous IL-1-induced CNS activation and the two monitored sickness symptoms. In addition, endothelial-specific knockdown of IL-1R1 blocked the induction of cyclooxygenase-2 expression induced by all three routes of IL-1 administration. These results show that the effects of intravenous and intracerebroventricular IL-1 are mediated by endothelial IL-1R1, whereas the effects of intraperitoneal IL-1 are partially dependent on endothelial IL-1R1.
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“The CNS is affected by cytokines (97), and this can occur through a variety of different mechanisms, including direct transfer across the BBB, stimulation of CVOs, nerve stimulation, or release from infiltrating or resident immune cells, among others (98).”
From The anatomy and immunology of vasculature in the central nervous system (2019) · cites this paper as [97]
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Metadata
- Authors
- Ching, S., Zhang, H., Belevych, N., He, L., Lai, W., Pu, X.-A., Jaeger, L. B., Chen, Q., & Quan, N.
- Affiliations
- The Ohio State University (US)
- Saint Louis University (US)
- Venue
- The Journal of Neuroscience
- Publisher
- Society for Neuroscience
- Published
- 2007-09-26
- Volume
- 27
- Issue
- 39
- Pages
- 10476-10486
- ISSN
- 0270-6474 (ISSN-L)
- 1529-2401
- PMC
- PMC6673171
- OpenAlex
- W2047319988
- Cited by
- 156
- References
- 39
- Citations by year
- 2026: 3 · 2025: 2 · 2024: 4 · 2023: 5 · 2022: 6 · 2021: 9 · 2020: 10 · 2019: 4 · 2018: 7 · 2017: 16 · 2016: 9 · 2015: 13 · 2014: 18 · 2013: 7 · 2012: 10
- FWCI
- 5.51
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Neuroscience (publishedVersion, cc-by-nc-sa)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health
- National Institute of Allergy and Infectious Diseases (R01AI059089)
- Topics
- Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
- Health Sciences › Medicine › Critical Care and Intensive Care Medicine › Thermal Regulation in Medicine
- Life Sciences › Neuroscience › Biological Psychiatry › Tryptophan and brain disorders
- Keywords
- interleukin-1, endothelial cells, neuroimmune interactions, hypothalamus, c-fos, paraventricular nucleus, fever, locomotor activity, leukocyte infiltration, cyclooxygenase-2, route of administration, intracerebroventricular administration, intraperitoneal injection, intravenous injection, sickness behavior
- MeSH terms
- Animals, Central Nervous System, Fever, Hypothalamus, Immunologic Factors, Interleukin-1, Mice, Transgenic, Motor Activity, Neuroimmunomodulation, Genes, fos, Endothelial Cells, Mice, Cyclooxygenase 2, Receptors, Interleukin-1 Type I
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