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Cited by 31Microtubule and mitosis dynamicsGenomics and Chromatin DynamicsDNA Repair Mechanisms

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Stable kinetochore‐microtubule attachment requires loop‐dependent Ndc80‐Ndc80 binding

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Polley, Soumitra, Müschenborn, Helen, Terbeck, Melina, De Antoni, Anna, Vetter, Ingrid R, Dogterom, Marileen, Musacchio, Andrea, Volkov, Vladimir A, Huis in 't Veld, Pim J·2023·The EMBO Journal
DOI 10.15252/embj.2022112504PMC PMC10308368

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Abstract

During cell division, kinetochores link chromosomes to spindle microtubules. The Ndc80 complex, a crucial microtubule binder, populates each kinetochore with dozens of copies. Whether adjacent Ndc80 complexes cooperate to promote microtubule binding remains unclear. Here we demonstrate that the Ndc80 loop, a short sequence that interrupts the Ndc80 coiled-coil at a conserved position, folds into a more rigid structure than previously assumed and promotes direct interactions between full-length Ndc80 complexes on microtubules. Mutations in the loop impair these Ndc80-Ndc80 interactions, prevent the formation of force-resistant kinetochore-microtubule attachments, and cause cells to arrest in mitosis for hours. This arrest is not due to an inability to recruit the kinetochore-microtubule stabilizing SKA complex and cannot be overridden by mutations in the Ndc80 tail that strengthen microtubule attachment. Thus, loop-mediated organization of adjacent Ndc80 complexes is crucial for stable end-on kinetochore-microtubule attachment and spindle assembly checkpoint satisfaction.

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Metadata

Authors
Polley, S., Müschenborn, H., Terbeck, M., De Antoni, A., Vetter, I. R., Dogterom, M., Musacchio, A., Volkov, V. A., & Huis in 't Veld, P. J.
Affiliations
  • Max Planck Institute of Molecular Physiology (DE)
  • Delft University of Technology (NL)
  • Queen Mary University of London (GB)
Corresponding author
  • Musacchio, Andrea
  • Volkov, Vladimir A
ORCID
Venue
The EMBO Journal
Publisher
Springer Science and Business Media LLC
Published
2023-05-19
Volume
42
Issue
13
Pages
e112504-e112504
ISSN
  • 0261-4189 (ISSN-L)
  • 1460-2075
DOI
10.15252/embj.2022112504
PMC
PMC10308368
OpenAlex
W4377094368
Cited by
31
References
2
Citations by year
2026: 8 · 2025: 6 · 2024: 8 · 2023: 9
FWCI
4.49
Type
journal article
Language
en
Open access
Licence
Locations
  • The EMBO Journal (publishedVersion, cc-by-nc-nd)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc)
  • PubMed Central (publishedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
  • Data Archiving and Networked Services (DANS) (submittedVersion, cc-by-nc-nd)
  • Queen Mary Research Online (Queen Mary University of London) (submittedVersion, cc-by-nc-nd)
  • Research Repository (Delft University of Technology) (submittedVersion)
Funders
  • EC | European Research Council (609822, 951430)
  • QMUL Startup Grant (SBC8VOL2)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
Keywords
Ndc80 complex, kinetochore-microtubule attachment, coiled coil, microtubule binding, spindle assembly checkpoint, mitotic arrest, chromosome segregation, mitosis
MeSH terms
Animals, Microtubule-Associated Proteins, Microtubules, Mitosis, Protein Binding, Kinetochores, Chromosome Segregation

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