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Systems‐wide analysis of BCR signalosomes and downstream phosphorylation and ubiquitylation
Open accessAbstract
B‐cell receptor (BCR) signaling is essential for the development and function of B cells; however, the spectrum of proteins involved in BCR signaling is not fully known. Here we used quantitative mass spectrometry‐based proteomics to monitor the dynamics of BCR signaling complexes (signalosomes) and to investigate the dynamics of downstream phosphorylation and ubiquitylation signaling. We identify most of the previously known components of BCR signaling, as well as many proteins that have not yet been implicated in this system. BCR activation leads to rapid tyrosine phosphorylation and ubiquitylation of the receptor‐proximal signaling components, many of which are co‐regulated by both the modifications. We illustrate the power of multilayered proteomic analyses for discovering novel BCR signaling components by demonstrating that BCR‐induced phosphorylation of RAB7A at S72 prevents its association with effector proteins and with endo‐lysosomal compartments. In addition, we show that BCL10 is modified by LUBAC‐mediated linear ubiquitylation, and demonstrate an important function of LUBAC in BCR‐induced NF‐κB signaling. Our results offer a global and integrated view of BCR signaling, and the provided datasets can serve as a valuable resource for further understanding BCR signaling networks.
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“Anti-IgM antibodies stimulate B cells through the B cell receptor (BCR) thereby activating further downstream signaling cascades like the phosphatidylinositol 3-kinase (PI3K), protein kinase B (AKT) and mitogen-activated protein kinase (MAPK) pathways [51–53].”
From The oncoprotein TBX3 is controlling severity in experimental arthritis (2019) · cites this paper as [53]
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Metadata
- Authors
- Satpathy, S., Wagner, S. A., Beli, P., Gupta, R., Kristiansen, T. A., Malinova, D., Francavilla, C., Tolar, P., Bishop, G. A., Hostager, B. S., & Choudhary, C.
- Affiliations
- University of Copenhagen (DK)
- National Institute for Medical Research (GB)
- University of Iowa (US)
- Corresponding author
- Choudhary, Chunaram
- ORCID
- Venue
- Molecular Systems Biology
- Published
- 2015-06-01
- Volume
- 11
- Issue
- 6
- Pages
- 810-810
- ISSN
- 1744-4292 (ISSN-L)
- PMC
- PMC4501846
- OpenAlex
- W1894912312
- Cited by
- 124
- References
- 92
- Citations by year
- 2026: 3 · 2025: 3 · 2024: 9 · 2023: 10 · 2022: 9 · 2021: 17 · 2020: 19 · 2019: 7 · 2018: 14 · 2017: 15 · 2016: 16 · 2015: 2
- FWCI
- 5.18
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Molecular Systems Biology (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Research at the University of Copenhagen (University of Copenhagen) (publishedVersion, other-oa)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- Figshare (submittedVersion, cc-by)
- Research Explorer (The University of Manchester) (submittedVersion)
- Funders
- EMBO long‐term Fellowship
- Novo Nordisk Foundation (NNF14CC0001)
- EMBO Young Investigator Program
- Sapere Aude Program
- Danish Council for Independent Research
- Biological Sciences Funding Program
- Medical Research Council UK (U117597138)
- LOEWE Program Ubiquitin Networks (Ub‐Net) of the State of Hesse
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › NF-κB Signaling Pathways
- Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
- Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
- Keywords
- B cell receptor signaling, signalosome, tyrosine phosphorylation, ubiquitination, LUBAC, Rab7, BCL10, NF-κB signaling, B cell development
- MeSH terms
- B-Cell CLL-Lymphoma 10 Protein, B-Lymphocytes, HeLa Cells, Humans, Membrane Proteins, Phosphorylation, Receptors, Antigen, B-Cell, Signal Transduction, NF-kappa B, Proteomics, Ubiquitin-Protein Ligases, Adaptor Proteins, Signal Transducing, Ubiquitination, HEK293 Cells
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