Impact of metformin and rosiglitazone treatment on glucose transporter 4 mRNA expression in women with polycystic ovary syndrome.

Jensterle, Mojca; Janez, Andrej; Mlinar, Barbara; Marc, Janja; Prezelj, Janez; Pfeifer, Marija

doi:10.1530/eje-07-0857

Cited by 47Ovarian function and disordersDiabetes, Cardiovascular Risks, and LipoproteinsMetabolism, Diabetes, and Cancer

Impact of metformin and rosiglitazone treatment on glucose transporter 4 mRNA expression in women with polycystic ovary syndrome.

Jensterle, Mojca, Janez, Andrej, Mlinar, Barbara, Marc, Janja, Prezelj, Janez, Pfeifer, Marija·2008·European Journal of Endocrinology

DOI 10.1530/eje-07-0857

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Abstract

Objective: The insulin-resistant state of the polycystic ovary syndrome (PCOS) was found to be associated with a decreased glucose transporter GLUT4 expression in the insulin target tissues. This study was performed to explore whether the well-known clinical, hormonal and metabolic efficacy of metformin or rosiglitazone treatment is reflected in the modulation of adipocyte GLUT4 mRNA expression in patients with PCOS. Methods: We enrolled 35 women with PCOS. They received either metformin or rosiglitazone for 6 months. A history, blood samples for the measurement of androgens and s.c. adipose tissue samples were taken at baseline and end point. Quantification of GLUT4 mRNA expression in adipose tissue was performed using real-time quantitative PCR. Homeostasis model assessment (HOMA IR) score calculation was applied as a measure for insulin resistance (IR). Results: GLUT4 mRNA expression in adipose tissue increased significantly in both groups (P<0.001). The increase was more pronounced in the rosiglitazone group (P=0.040). There was a statistically significant improvement of HOMA IR in both groups (P=0.008). After treatment, frequencies of menstrual bleeding were significantly higher (P<0.001) and serum total testosterone levels significantly lower in both groups (P=0.001). Conclusions: A 6-month therapy with insulin sensitizers resulted in marked improvement in adipose tissue GLUT4 mRNA expression in PCOS patients, rosiglitazone being more effective when compared with metformin. The augmentation of the insulin signal transduction was accompanied by a significant improvement of HOMA IR, menstrual pattern and androgen profile.

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Metformin enhances expression of GLUT4 mRNA in the adipose tissue of patients with PCOS.
“In fact, metformin has shown to enhance expression of GLUT4 mRNA in the adipose tissue of patients with PCOS [44] (which was found to be significantly lower compared to healthy controls).”
From From Prediabetes to Type 2 Diabetes Mellitus in Women with Polycystic Ovary Syndrome: Lifestyle and Pharmacological Management (2020) · cites this paper as [44]

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Authors: Jensterle, M., Janez, A., Mlinar, B., Marc, J., Prezelj, J., & Pfeifer, M.
Affiliations: Ljubljana University Medical Centre (SI)
University of Ljubljana (SI)
Corresponding author: Pfeifer, Marija
ORCID: Jensterle, Mojca
Marc, Janja
Venue: European Journal of Endocrinology
Publisher: Oxford University Press (OUP)
Published: 2008-06
Volume: 158
Issue: 6
Pages: 793-801
ISSN: 0804-4643 (ISSN-L)
1479-683X
DOI: 10.1530/eje-07-0857
OpenAlex: W2164520220
Cited by: 47
References: 61
Citations by year: 2025: 2 · 2024: 2 · 2023: 5 · 2022: 2 · 2021: 4 · 2020: 2 · 2019: 1 · 2018: 1 · 2017: 1 · 2016: 1 · 2015: 1 · 2014: 2 · 2013: 4 · 2012: 3
FWCI: 6.18
Type: journal article
Language: en
Open access: closed
Locations: European Journal of Endocrinology (publishedVersion)
PubMed (publishedVersion)
Topics: Health Sciences › Medicine › Reproductive Medicine › Ovarian function and disorders
Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Diabetes, Cardiovascular Risks, and Lipoproteins
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolism, Diabetes, and Cancer
Keywords: polycystic ovary syndrome, metformin, rosiglitazone, glucose transporter 4, adipose tissue, insulin resistance, insulin sensitizers, insulin signaling, menstrual bleeding, androgens, testosterone, HOMA-IR
MeSH terms: Rosiglitazone, Adolescent, Adult, Analysis of Variance, Androgens, Female, Humans, Hypoglycemic Agents, Insulin Resistance, Metformin, Polycystic Ovary Syndrome, RNA, Messenger, Time Factors, Gene Expression, Reverse Transcriptase Polymerase Chain Reaction, Thiazolidinediones, Glucose Transporter Type 4

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Impact of metformin and rosiglitazone treatment on glucose transporter 4 mRNA expression in women with polycystic ovary syndrome.

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