The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
The Carnegie Protein Trap Library: A Versatile Tool for Drosophila Developmental Studies
Open accessAbstract
Metazoan physiology depends on intricate patterns of gene expression that remain poorly known. Using transposon mutagenesis in Drosophila, we constructed a library of 7404 protein trap and enhancer trap lines, the Carnegie collection, to facilitate gene expression mapping at single-cell resolution. By sequencing the genomic insertion sites, determining splicing patterns downstream of the enhanced green fluorescent protein (EGFP) exon, and analyzing expression patterns in the ovary and salivary gland, we found that 600–900 different genes are trapped in our collection. A core set of 244 lines trapped different identifiable protein isoforms, while insertions likely to act as GFP-enhancer traps were found in 256 additional genes. At least 8 novel genes were also identified. Our results demonstrate that the Carnegie collection will be useful as a discovery tool in diverse areas of cell and developmental biology and suggest new strategies for greatly increasing the coverage of the Drosophila proteome with protein trap insertions.
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Cites
Global analysis of protein localization in budding yeast
Huh, W.-K., Falvo, J. V., Gerke, L. C., Carroll, A. S., Howson, R. W., Weissman, J. S., & O'Shea, E. K. (2003) · Nature · doi:10.1038/nature02026 · Cited by 4,248 · cited 3×
“The great potential of this technology has been extensively documented in yeast, where large collections of strains that each trap a different gene have been generated using transposable elements (Ross-Macdonald et al. 1999) or by homologous recombination (Huh et al. 2003).”
Background
Cited by
Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution
Chen, B.-C., Legant, W. R., Wang, K., Shao, L., Milkie, D. E., Davidson, M. W., Janetopoulos, C., Wu, X. S., Hammer, J. A., Liu, Z., et al. (2014) · Science · doi:10.1126/science.1257998 · Cited by 1,919
Fluorescent fusion protein knockout mediated by anti-GFP nanobody
Caussinus, E., Kanca, O., & Affolter, M. (2012) · Nature Structural & Molecular Biology · doi:10.1038/nsmb.2180 · Cited by 510
Wirtz-Peitz, F., Nishimura, T., & Knoblich, J. A. (2008) · Cell · doi:10.1016/j.cell.2008.07.049 · Cited by 369
Passenger deletions generate therapeutic vulnerabilities in cancer
Muller, F. L., Colla, S., Aquilanti, E., Manzo, V. E., Genovese, G., Lee, J., Eisenson, D., Narurkar, R., Deng, P., Nezi, L., et al. (2012) · Nature · doi:10.1038/nature11331 · Cited by 339
A genome-wide resource for the analysis of protein localisation in Drosophila
Sarov, M., Barz, C., Jambor, H., Hein, M. Y., Schmied, C., Suchold, D., Stender, B., Janosch, S., KJ, V. V., Krishnan, R., et al. (2016) · eLife · doi:10.7554/elife.12068 · Cited by 359
Metadata
- Authors
- Buszczak, M., Paterno, S., Lighthouse, D., Bachman, J., Planck, J., Owen, S., Skora, A. D., Nystul, T. G., Ohlstein, B., Allen, A., Wilhelm, J. E., Murphy, T. D., Levis, R. W., Matunis, E., Srivali, N., Hoskins, R. A., & Spradling, A. C.
- Venue
- Genetics
- Publisher
- Oxford University Press (OUP)
- Published
- 2007-03-01
- Volume
- 175
- Issue
- 3
- Pages
- 1505-1531
- ISSN
- 0016-6731 (ISSN-L)
- 1943-2631
- OpenAlex
- W2103046819
- Cited by
- 577
- References
- 1
- FWCI
- 6.75
- Type
- journal article
- Language
- en
- Open access
- green
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- Buszczak, M., Paterno, S., Lighthouse, D., Bachman, J., Planck, J., Owen, S., Skora, A. D., Nystul, T. G., Ohlstein, B., Allen, A., Wilhelm, J. E., Murphy, T. D., Levis, R. W., Matunis, E., Srivali, N., Hoskins, R. A., & Spradling, A. C. (2007). The Carnegie Protein Trap Library: A Versatile Tool for Drosophila Developmental Studies. Genetics. https://doi.org/10.1534/genetics.106.065961
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