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Cited by 9Hair Growth and DisordersNeuropeptides and Animal PhysiologyThermoregulation and physiological responses

Cholinergic- rather than adrenergic-induced sweating play a role in developing and developed rat eccrine sweat glands

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Zhang, Lei, Zhang, Xiang, Du, Lijie, Zhang, Cuiping, Li, Haihong·2021·Experimental Animals
DOI 10.1538/expanim.20-0144PMC PMC8150243
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Abstract

Both cholinergic and adrenergic stimulation can induce sweat secretion in human eccrine sweat glands, but whether cholinergic and adrenergic stimulation play same roles in rat eccrine sweat glands is still controversial. To explore the innervations, and adrenergic- and cholinergic-induced secretory response in developing and developed rat eccrine sweat glands, rat hind footpads from embryonic day (E) 15.5-20.5, postanal day (P) 1-14, P21 and adult were fixed, embedded, sectioned and subjected to immunofluorescence staining for general fiber marker protein gene product 9.5 (PGP 9.5), adrenergic fiber marker tyrosine hydroxylase (TH) and cholinergic fiber marker vasoactive intestinal peptide (VIP), and cholinergic- and adrenergic-induced sweat secretion was detected at P1-P21 and adult rats by starch-iodine test. The results showed that eccrine sweat gland placodes of SD rats were first appeared at E19.5, and the expression of PGP 9.5 was detected surrounding the sweat gland placodes at E19.5, TH at P7, and VIP at P11. Pilocarpine-induced sweat secretion was first detected at P16 in hind footpads by starch-iodine test. There was no measurable sweating when stimulated by alpha- or beta-adrenergic agonists at all the examined time points. We conclude that rat eccrine sweat glands, just as human eccrine sweat glands, co-express adrenergic and cholinergic fibers, but different from human eccrine sweat glands, cholinergic- rather than adrenergic-induced sweating plays a role in the developing and developed rat eccrine sweat glands.

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Cited by

Sorted by citations

Authors
Zhang, L., Zhang, X., Du, L., Zhang, C., & Li, H.
Affiliations
  • Hubei University of Medicine (CN)
  • Chinese PLA General Hospital (CN)
ORCID
Venue
Experimental Animals
Publisher
Japanese Association for Laboratory Animal Science
Published
2021
Volume
70
Issue
2
Pages
218-224
ISSN
  • 0007-5124 (ISSN-L)
  • 1341-1357
  • 1881-7122
DOI
10.1538/expanim.20-0144
PMC
PMC8150243
OpenAlex
W3110856897
Cited by
9
References
1
Citations by year
2026: 2 · 2025: 2 · 2024: 1 · 2023: 3 · 2022: 1
FWCI
1.14
Type
journal article
Language
en
Open access
Locations
  • EXPERIMENTAL ANIMALS (publishedVersion)
  • PubMed Central (publishedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
Funders
National Natural Science Foundation of China (81471882, 81772102)
Topics
  • Health Sciences › Medicine › Urology › Hair Growth and Disorders
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
  • Health Sciences › Medicine › Physiology › Thermoregulation and physiological responses
Keywords
eccrine sweat glands, sweat gland development, cholinergic innervation, adrenergic innervation, sweat secretion, vasoactive intestinal peptide, tyrosine hydroxylase, PGP 9.5, alpha-adrenergic agonists, beta-adrenergic agonists, rat embryo
MeSH terms
Animals, Eccrine Glands, Female, Male, Sweating, Rats, Sprague-Dawley, Adrenergic Agents, Cholinergic Agents, Rats

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