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Transgenic Mouse Sperm that Have Green Acrosome and Red Mitochondria Allow Visualization of Sperm and Their Acrosome Reaction in Vivo

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HASUWA, Hidetoshi, MURO, Yuko, IKAWA, Masahito, KATO, Noriko, TSUJIMOTO, Yoshihide, OKABE, Masaru·2010·Experimental Animals

Abstract

In the present paper, we introduce a transgenic mouse line whose sperm express green fluorescent protein (GFP) in their acrosome and red fluorescent protein (RFP) in their mitochondria [B6D2F1- Tg(CAG/su9-DsRed2, Acr3-EGFP)RBGS002Osb]. The dual fluorescent sperm showed normal fertilizing ability in both in vivo and in vitro fertilization and the sperm could be observed through uterine and oviductal walls when female reproductive tracts were dissected out and placed under excitation light. This characteristic could facilitate examination of sperm migration inside the female reproductive tract as well as facilitating in situ live imaging of the acrosome reaction, the details of which have remained elusive.

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Metadata

Authors
HASUWA, H., MURO, Y., IKAWA, M., KATO, N., TSUJIMOTO, Y., & OKABE, M.
Venue
Experimental Animals
Published
2010
Volume
59
Issue
1
Pages
105-107
ISSN
  • 0007-5124 (ISSN-L)
  • 1341-1357
  • 1881-7122
OpenAlex
W2067633155
Cited by
158
References
0
FWCI
5.53
Type
journal article
Language
en
Open access
bronze

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CoChat. (2026). Citation check: Transgenic Mouse Sperm that Have Green Acrosome and Red Mitochondria Allow Visualization of Sperm and Their Acrosome Reaction in Vivo. https://app.cochat.ai/papers/10.1538/expanim.59.105
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HASUWA, H., MURO, Y., IKAWA, M., KATO, N., TSUJIMOTO, Y., & OKABE, M. (2010). Transgenic Mouse Sperm that Have Green Acrosome and Red Mitochondria Allow Visualization of Sperm and Their Acrosome Reaction in Vivo. Experimental Animals. https://doi.org/10.1538/expanim.59.105

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