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Transgenic Mouse Sperm that Have Green Acrosome and Red Mitochondria Allow Visualization of Sperm and Their Acrosome Reaction in Vivo
Open accessAbstract
In the present paper, we introduce a transgenic mouse line whose sperm express green fluorescent protein (GFP) in their acrosome and red fluorescent protein (RFP) in their mitochondria [B6D2F1- Tg(CAG/su9-DsRed2, Acr3-EGFP)RBGS002Osb]. The dual fluorescent sperm showed normal fertilizing ability in both in vivo and in vitro fertilization and the sperm could be observed through uterine and oviductal walls when female reproductive tracts were dissected out and placed under excitation light. This characteristic could facilitate examination of sperm migration inside the female reproductive tract as well as facilitating in situ live imaging of the acrosome reaction, the details of which have remained elusive.
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Citations
Cited by
Rheotaxis Guides Mammalian Sperm
Miki, K., & Clapham, D. E. (2013) · Current Biology · doi:10.1016/j.cub.2013.02.007 · Cited by 444
Jin, M., Fujiwara, E., Kakiuchi, Y., Okabe, M., Satouh, Y., Baba, S. A., Chiba, K., & Hirohashi, N. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1018202108 · Cited by 422
Self-citationCalcium Channels in the Development, Maturation, and Function of Spermatozoa
Darszon, A., Nishigaki, T., Beltran, C., & Treviño, C. L. (2011) · Physiological Reviews · doi:10.1152/physrev.00028.2010 · Cited by 364
Unique insights into maternal mitochondrial inheritance in mice
Luo, S.-M., Ge, Z.-J., Wang, Z.-W., Jiang, Z.-Z., Wang, Z.-B., Ouyang, Y.-C., Hou, Y., Schatten, H., & Sun, Q.-Y. (2013) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1303231110 · Cited by 153
Platelets release mitochondrial antigens in systemic lupus erythematosus
Melki, I., Allaeys, I., Tessandier, N., Lévesque, T., Cloutier, N., Laroche, A., Vernoux, N., Becker, Y., Benk-Fortin, H., Zufferey, A., et al. (2021) · Science Translational Medicine · doi:10.1126/scitranslmed.aav5928 · Cited by 125
Metadata
- Authors
- HASUWA, H., MURO, Y., IKAWA, M., KATO, N., TSUJIMOTO, Y., & OKABE, M.
- Venue
- Experimental Animals
- Published
- 2010
- Volume
- 59
- Issue
- 1
- Pages
- 105-107
- ISSN
- 0007-5124 (ISSN-L)
- 1341-1357
- 1881-7122
- OpenAlex
- W2067633155
- Cited by
- 158
- References
- 0
- FWCI
- 5.53
- Type
- journal article
- Language
- en
- Open access
- bronze
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- CoChat. (2026). Citation check: Transgenic Mouse Sperm that Have Green Acrosome and Red Mitochondria Allow Visualization of Sperm and Their Acrosome Reaction in Vivo. https://app.cochat.ai/papers/10.1538/expanim.59.105
- The paper
- HASUWA, H., MURO, Y., IKAWA, M., KATO, N., TSUJIMOTO, Y., & OKABE, M. (2010). Transgenic Mouse Sperm that Have Green Acrosome and Red Mitochondria Allow Visualization of Sperm and Their Acrosome Reaction in Vivo. Experimental Animals. https://doi.org/10.1538/expanim.59.105
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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