Polygonum hydropiper extract attenuates ethanol-induced gastric damage through antioxidant and anti-inflammatory pathways
Open accessAbstract
The present study was conducted to investigate the underlying mechanisms and effective components of Polygonum hydropiper in ethanol-induced acute gastric mucosal lesions. The ethanol extract was purified on an AB-8 macroporous resin column and eluted with 60% ethanol and was then injected into the HPLC system for quantitative analysis. Sprague-Dawley rats were orally pretreated with P. hydropiper extract (PHLE; 50, 100, and 200 mg/kg) for 5 days and then absolute ethanol was administered to induce gastric mucosal damage. One hour after ethanol ingestion, the rats were euthanized and stomach samples were collected for biochemical analysis. Antioxidant enzymes and anti-inflammatory cytokines were quantified. Western blotting was used to detect the expression levels of proteins. Cell proliferation was assayed by CCK-8 assays. The proportion of total flavonoids in the final extract of P. hydropiper was 50.05%, which contained three major bioactive flavonoid constituents, including rutin, quercitrin, and quercetin. PHLE significantly increased cell viability and effectively protected human gastric epithelial cells-1 against alcohol-induced damage in vitro. PHLE pretreatment attenuated gastric mucosal injuries in a dose-dependent manner in rats, and increased the activity of superoxide dismutase, glutathione peroxidase, and glutathione, and decreased the levels of malondialdehyde in gastric tissue. Pretreatment with PHLE also reduced the generation of the pro-inflammatory cytokines tumor necrosis factor-α and interleukin-1β in gastric tissue by downregulating the expression of nuclear factor-kappa B. PHLE exerted protective effects against gastric injury through antioxidant and anti-inflammatory pathways. Flavonoids might be the main effective components of P. hydropiper against gastric mucosal injury.
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Phenolic chemicals have health advantages including prevention of cardiovascular disease, cancer, diabetes and obesity.
“Some of the health advantages associated with phenolic chemicals include the prevention of cardiovascular disease, cancer, diabetes and obesity [99, 100].”
From Functional, nutritional and medicinal potential of banana peel (2023) · cites this paper as [100]
The Discussion describes antioxidant and anti-inflammatory activity of flavonoids, and the Results identify flavonoids in the extract; neither addresses prevention of cardiovascular disease, cancer, diabetes, or obesity. Searches for those diseases alongside phenolic chemicals or compounds found no relevant passage in our copy. (LLM, conf=0.76)
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- Authors
- Ren, S., Chen, B., Ma, Z., Hu, H., & Xie, Y.
- Affiliations
- Hainan Medical University (CN)
- Hainan University (CN)
- Venue
- Brazilian Journal of Medical and Biological Research
- Publisher
- FapUNIFESP (SciELO)
- Published
- 2021
- Volume
- 54
- Issue
- 8
- Pages
- e10841-e10841
- ISSN
- 0100-879X (ISSN-L)
- 1414-431X
- PMC
- PMC8148888
- OpenAlex
- W3161585325
- Cited by
- 19
- References
- 34
- Citations by year
- 2026: 3 · 2025: 3 · 2024: 5 · 2023: 3 · 2022: 4 · 2021: 1
- FWCI
- 3.66
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Brazilian Journal of Medical and Biological Research (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (publishedVersion, other-oa)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- National Natural Science Foundation of China (81760788)
- Natural Science Foundation of Hainan Province
- Topics
- Health Sciences › Medicine › Complementary and alternative medicine › Medicinal plant effects and applications
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Medicinal Plant Research
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Phytochemistry and biological activity of medicinal plants
- Keywords
- Polygonum hydropiper, ethanol-induced gastric injury, gastroprotection, flavonoids, rutin, quercetin, quercitrin, antioxidant enzymes, oxidative stress, inflammatory cytokines, NF-κB, gastric epithelial cells, plant extracts
- MeSH terms
- Ethanol, Animals, Anti-Inflammatory Agents, Antioxidants, Gastric Mucosa, Plant Extracts, Rats, Sprague-Dawley, Polygonum, Rats
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