Serosurvey of Tick-Borne Pathogens in Dogs from Urban and Rural Areas from Parana State, Brazil
Open accessAbstract
Considering the zoonotic potential of tick-borne disease (TBD) agents and the fact that dogs may act as sentinels for human infection, the aim of the present study was to determine the seroprevalence of TBD agents and risk factors for exposure in two different canine populations from Parana State, Southern Brazil. A total of 138 dog serum samples from urban (UA) (n=68) and rural (RA) (n=70) areas were tested with commercial ELISA rapid test forAnaplasma phagocytophilum, Ehrlichia canisand Borrelia burgdorferi antibodies and indirect immunofluorescence assay (IFAT) for Babesia vogeli. An overall of 92∕138 (66.7%) dogs, being 62∕68 (91.2%) from UA and 30∕70 (42.9%) from RA, were seropositive for at least one TBD agent. From the total number of dogs, sixty-two were positive for E. canis (44.9%), 19 (13.8%) for A. phagocytophilum, and 64 (46.4%) for B. vogeli. Anti-B. burgdorferi antibodies were not detected. Dogs from UA showed a higher percentage of tick infestation (p = 0.0135) and were highly associated with seropositivity to E. canis (p = 0.000005), A. phagocytophilum (p = 0.0001), and B. vogeli (p = 0.0012). In summary, the findings indicate that dogs from urban areas present higher potential risk exposure to TBD pathogens than those from rural areas.
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Dogs in urban areas in Brazil harbour more tick-borne pathogens than rural dogs.
“For example, the prevalence of parvovirus increases with proximity to urban areas in grey foxes (Urocyon cinereoargenteus) in the USA (Riley, Foley, and Chomel 2004), and dogs in urban areas in Brazil harbour more tick-borne pathogens than rural dogs (Vieira et al. 2013).”
From Red fox viromes in urban and rural landscapes (2020) · cites this paper as Vieira et al. 2013
The Discussion reports that 91.2% of sampled urban dogs were seropositive for at least one tick-borne disease agent and were 13.7 times more likely to be seropositive than rural dogs. But the study sampled dogs in one region of Paraná and measured antibodies, so it does not establish that urban dogs across Brazil harbour more pathogens. (LLM, conf=0.87)
Experimental — accuracy not yet measuredUrbanisation influences pathogen exposure and prevalence in wildlife.
“Urbanisation influences pathogen exposure and prevalence in wildlife.”
From Red fox viromes in urban and rural landscapes (2020) · cites this paper as Vieira et al. 2013
The Results report that urban residence was associated with higher seropositivity to three tick-borne pathogens, and the Discussion reports higher overall seroprevalence in urban than rural dogs. The study concerns domestic dogs, not wildlife, and compares locations rather than demonstrating an effect of urbanisation itself. (LLM, conf=0.86)
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References 29 · Cited by 48
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- Abel IS, Marzagão G, Yoshinari NH, Schumaker TTS. Borrelia-like spirochetes recovered from ticks and small mammals collected in the Atlantic forest reserve, Cotia County, State of São Paulo, Brazil. Mem Inst Oswaldo Cruz 2000; 95(5): 621-624. PMid:10998210. http://dx.doi. org/10.1590/S0074-02762000000500006 · DOI
- Amusategui I, Tesouro MA, Kakoma I, Sainz A. Serological reactivity to Ehrlichia canis, Anaplasma phagocytophilum, Neorickettsia risticii, Borrelia burgdorferi and Rickettsia conorii in dogs from Northwestern Spain. Vector Borne Zoonotic Dis 2008; 8(6): 797-803. PMid:18597660. http://dx.doi. org/10.1089/vbz.2007.0277 · DOI · Paper page
- Aragão HB, Fonseca F. Notas de Ixodologia: VIII. Lista e chave para os representantes da fauna ixodológica brasileira. Mem Inst Oswaldo Cruz 1961; 59(2): 115-129. PMid:13861962. http://dx.doi. org/10.1590/S0074-02761961000200001 · DOI
- Barros-Battesti DM, Yoshinari NH, Bonoldi VLN, Gomes AC. Parasitism by Ixodes didelphidis and I. loricatus (Acari: Ixodidae) on small wild mammals from an Atlantic forest in the State of Sao Paulo, Brazil. J Med Entomol 2000; 37(6): 820-827. PMid:11185614. http://dx.doi. org/10.1603/0022-2585-37.6.820 · DOI
- Bowman D, Little SE, Lorentzen L, Shields J, Sullivan MP, Carlin EP. Prevalence and geographic distribution of Dirofilaria immitis, Borrelia burgdorferi, Ehrlichia canis, and Anaplasma phagocytophilum in dogs in the United States: Results of a national clinic-based serologic survey. Vet Parasitol 2009; 160(1-2): 138-48. PMid:19150176. http://dx.doi. org/10.1016/j.vetpar.2008.10.093 · DOI
- Carlos RSA, Muniz Neta ES, Spagnol FH, Oliveira LLS, Brito RLL, Albuquerque GR, et al. Frequência de anticorpos anti-Erhlichia canis, Borrelia burgdorferi e antígenos de Dirofilaria immitis em cães na microrregião Ilhéus-Itabuna, Bahia, Brasil. Rev Bras Parasitol Vet 2007; 16(3): 117-120. PMid:18078596. http://dx.doi.org/10.1590/ S1984-29612007000300001 · DOI
- Chandrashekar R, Mainville CA, Beall MJ, O'Connor T, Eberts MD, Alleman AR, et al. Performance of a commercially available in-clinic ELISA for the detection of antibodies against Anaplasma phagocytophilum, Ehrlichia canis, and Borrelia burgdorferi and Dirofilaria immitis antigen in dogs. Am J Vet Res 2010; 71(12): 1443-50. PMid:21117995. http:// dx.doi.org/10.2460/ajvr.71.12.1443 · DOI
- Couto CG, Lorentzen L, Beall MJ, Shields J, Bertolone N, Couto JI, et al. Serological study of selected vector-borne diseases in shelter dogs in Central Spain using point-of-care assays. Vector Borne Zoonotic Dis 2010; 10(9): 885-888. PMid:20420531. http://dx.doi.org/10.1089/ vbz.2009.0063 · DOI · Paper page
- Dagnone AS, Souza AI, André MR, Machado RZ. Molecular diagnosis of Anaplasmataceae organisms in dogs with clinical and microscopical signs of ehrlichiosis. Rev Bras Parasitol Vet 2009; 18(4): 20-25. PMid:20040204. http://dx.doi.org/10.4322/rbpv.01804004 · DOI
- Dantas-Torres F. Canine vector-borne diseases in Brazil. Parasit Vectors 2008; 1(1): 25. PMid:18691408 PMCid:2533296. http://dx.doi. org/10.1186/1756-3305-1-25 · DOI
- Ferreira RF, Cerqueira AMF, Pereira AM, Velho PB, Azevedo RRM, Rodrigues ILD, et al. Avaliação da ocorrência de reação cruzada em cães PCR-positivos para Anaplasma platys testados em ELISA comercial para detecção de anticorpos de Anaplasma phagocytophilum. Rev Bras Parasitol Vet 2008; 17(S1): 5-8. PMid:20059806.
- Figueiredo LT, Badra SJ, Pereira LE, Szabo MP. Report on ticks collected in the Southeast and Mid-West regions of Brazil: analyzing the potential transmission of tick-borne pathogens to man. Rev Soc Bras Med Trop 1999; 32(6): 613-619. PMid:10881097. http://dx.doi.org/10.1590/ S0037-86821999000600002 · DOI
- Furuta PI, Oliveira TM, Teixeira MC, Rocha AG, Machado RZ, Tinucci-Costa MG. Comparison between a soluble antigen-based ELISA and IFAT in detecting antibodies against Babesia canis in dogs. Rev Bras Parasitol Vet 2009; 18(3): 41-45. PMid:19772774. http://dx.doi. org/10.4322/rbpv.01803007 · DOI
- Labarthe N, Pereira MC, Barbarini O, McKee W, Coimbra CA, Hoskins J. Serologic prevalence of Dirofilaria immitis, Ehrlichia canis, and Borrelia burgdorferi infections in Brazil. Vet Therapeutics 2003; 4(1): 67-75. PMid:12756637.
- Labruna MB, Souza SLP, Guimarães Junior JS, Pacheco RC, Pinter A, Gennari SM. Prevalência de carrapatos em cães de áreas rurais da região norte do Estado do Paraná. Arq Bras Med Vet Zootec 2001, 53(5): 553- 556. http://dx.doi.org/10.1590/S0102-09352001000500007 · DOI
- Maia MG, Costa RT, Haddad JP, Passos LM, Ribeiro MF. Epidemiological aspects of canine babesiosis in the semiarid area of the state of Minas Gerais, Brazil. Prev Vet Med 2007; 79(2-4): 155-62. PMid:17210194. http://dx.doi.org/10.1016/j.prevetmed.2006.11.013 · DOI
- O'Connor TP, Hanscom JL, Hegarty BC, Groat RG, Breitschwerdt EB. Comparison of an indirect immunofluorescence assay, western blot analysis, and a commercially available ELISA for detection of Ehrlichia canis antibodies in canine sera. Am J Vet Res 2006; 67(2): 206-210. PMid:16454622. http://dx.doi.org/10.2460/ajvr.67.2.206 · DOI
- Ramos R, Ramos C, Araújo F, Oliveira R, Souza I, Pimentel D, et al. Molecular survey and genetic characterization of tick-borne pathogens in dogs in metropolitan Recife (north-eastern Brazil). Parasitol Res 2010; 107(5): 1115-1120. PMid:20680344. http://dx.doi. org/10.1007/s00436-010-1979-7 · DOI
- Santos F, Coppede JS, Pereira AL, Oliveira LP, Roberto PG, Benedetti RB, et al. Molecular evaluation of the incidence of Ehrlichia canis, Anaplasma platys and Babesia spp. in dogs from Ribeirão Preto, Brazil. Vet J 2009; 179(1): 145-148. PMid:17920967. http://dx.doi.org/10.1016/j. tvjl.2007.08.017 · DOI
- Santos HA, Pires MS, Vilela JAR, Santos TM, Faccini JLH, Baldani CD, et al. Detection of Anaplasma phagocytophilum in Brazilian dogs by real-time polymerase chain reaction. J Vet Diagn Invest 2011; 23(4): 770-774. PMid:21908321. http://dx.doi. org/10.1177/1040638711406974 · DOI
- Spolidorio MG, Labruna MB, Machado RZ, Moraes-Filho J, Zago AM, Donatele DM, et al. Survey for tick-borne zoonoses in the State of Espirito Santo, Southeastern Brazil. Am J Trop Med Hyg 2010; 83(1): 201-206. PMid:20595502 PMCid:2912600. http://dx.doi.org/10.4269/ ajtmh.2010.09-0595 · DOI
- Trapp SM, Dagnone AS, Vidotto O, Freire RL, Amude AM, Morais HS. Seroepidemiology of canine babesiosis and ehrlichiosis in a hospital population. Vet Parasitol 2006; 140(3-4): 223-230. PMid:16647817. http://dx.doi.org/10.1016/j.vetpar.2006.03.030 · DOI
- Uspensky I, Ioffe-Uspensky I. The dog factor in brown dog tick Rhipicephalus sanguineus (Acari: Ixodidae) infestations in and near human dwellings. Int J Med Microbiol 2002; 291(S33): 156-163. http://dx.doi. org/10.1016/S1438-4221(02)80030-3 · DOI
- Vieira RFC, Biondo AW, Guimarães AMS, Santos AP, Santo RP, Dutra LH, et al. Ehrlichiosis in Brazil. Rev Bras Parasitol Vet 2011; 20(1): 1-12. PMid:21439224. http://dx.doi.org/10.1590/ S1984-29612011000100002 Woldehiwet Z. The natural history of Anaplasma phagocytophilum. Vet Parasitol 2010; 167(2-4): 108-22. PMid:19811878. http://dx.doi. org/10.1016/j.vetpar.2009.09.013 · DOI
- Wong SS, Teng JL, Poon RW, Choi GK, Chan KH, Yeung ML, et al. Comparative evaluation of a point-of-care immunochromatographic test SNAP 4Dx with molecular detection tests for vector- borne canine pathogens in Hong Kong. Vector Borne Zoonotic Dis 2011; 11(9): 1269-1277. PMid:21612526. http://dx.doi. org/10.1089/vbz.2010.0265 · DOI
- Yoshinari NH, Abrão MG, Bonoldi VL, Soares CO, Madruga CR, Scofield A, et al. Coexistence of antibodies to tick-borne agents of babesiosis and Lyme borreliosis in patients from Cotia county, State of São Paulo, Brazil. Mem Inst Oswaldo Cruz 2003; 98(3): 311-318. PMid:12886408. http:// dx.doi.org/10.1590/S0074-02762003000300004 · DOI
- Authors
- Vieira, T. S. W. J., Vieira, R. F. D. C., Nascimento, D. A. G. D., Tamekuni, K., Toledo, R. D. S., Chandrashekar, R., Marcondes, M., Biondo, A. W., & Vidotto, O.
- Affiliations
- Universidade Estadual de Londrina (BR)
- Universidade Federal da Paraíba (BR)
- Universidade Estadual de Londrina – UEL, Brazil
- IDEXX Laboratories (United States) (US)
- Universidade Estadual Paulista (Unesp) (BR)
- University of Illinois Urbana-Champaign (US)
- ORCID
- Venue
- Revista Brasileira de Parasitologia Veterinária
- Publisher
- FapUNIFESP (SciELO)
- Published
- 2013-03
- Volume
- 22
- Issue
- 1
- Pages
- 104-109
- ISSN
- 0103-846X (ISSN-L)
- 1984-2961
- OpenAlex
- W2165127446
- Cited by
- 48
- References
- 29
- Citations by year
- 2026: 2 · 2025: 2 · 2024: 4 · 2023: 2 · 2022: 3 · 2021: 4 · 2020: 7 · 2019: 2 · 2018: 3 · 2017: 6 · 2016: 3 · 2015: 4 · 2014: 3 · 2013: 3
- FWCI
- 3.67
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by
- Locations
- Revista Brasileira de Parasitologia Veterinária/Brazilian Journal of Veterinary Parasitology (publishedVersion, cc-by)
- PubMed (publishedVersion)
- LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (publishedVersion, other-oa)
- Acervo Digital da Universidade Estadual Paulista (Universidade Estadual Paulista) (submittedVersion, other-oa)
- Americanae (AECID Library) (submittedVersion, other-oa)
- Redalyc (Universidad Autónoma del Estado de México) (submittedVersion)
- LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (submittedVersion, other-oa)
- Funders
- Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
- Fundação Araucária
- Topics
- Life Sciences › Immunology and Microbiology › Parasitology › Vector-borne infectious diseases
- Life Sciences › Immunology and Microbiology › Parasitology › Bartonella species infections research
- Health Sciences › Veterinary › Small Animals › Brucella: diagnosis, epidemiology, treatment
- Keywords
- tick-borne diseases, dogs, seroprevalence, Ehrlichia canis, Anaplasma phagocytophilum, Babesia vogeli, Borrelia burgdorferi, tick infestation, urban-rural comparison, zoonotic risk, Paraná
- MeSH terms
- Animals, Antibodies, Bacterial, Brazil, Dog Diseases, Dogs, Rural Health, Ticks, Urban Health, Seroepidemiologic Studies, Tick-Borne Diseases
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