Serological Study of Selected Vector-Borne Diseases in Shelter Dogs in Central Spain Using Point-of-Care Assays
Abstract
We evaluated the prevalence of selected vector-borne diseases in 131 dogs in an animal shelter in central Spain using point-of-care assays (SNAP 4DX and SNAP Leishmania; IDEXX Laboratories, Westbrook, ME). The SNAP 4DX detects Dirofilaria immitis ( Di ) antigen and antibodies against Ehrlichia canis ( Ec ), Borrelia burgdorferi ( Bb ), and Anaplasma phagocytophylum ( Aph ); the SNAP Leishmania kit detects antibodies against Leishmania infantum ( Li ). Dogs were classified as healthy or sick based on physical examination, complete blood counts, and serum chemistry profiles. The prevalence of positive test results was as follows: Ec , 5.3% ( n = 7); Aph , 19.0% ( n = 25); Bb , 0%; Di , 0%; and Li , 5.3% ( n = 7). Four dogs (3%) were coexposed to Ec and Aph , and three dogs (2.3%) were coexposed to Aph and Li . There was no statistically significant correlation between positive serology and clinical status (sick vs. healthy) or hematologic/biochemical abnormalities. The prevalence of Aph was the highest and is in agreement with a recent report in a dog shelter in northwestern Spain. These point-of-care assays may be more valuable as epidemiologic than as clinical tools.
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This study is the most complete survey of CVBD-agent seropositivity conducted in Spain.
“This study is the most complete survey of CVBD-agent seropositivity conducted in Spain.”
From Seropositivity rates for agents of canine vector-borne diseases in Spain: A multicentre study (2013) · cites this paper as [21]
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- Authors
- Couto, C. G., Lorentzen, L., Beall, M. J., Shields, J., Bertolone, N., Couto, J. I., Couto, K. M., Nash, S., Slack, J., Kvitko, H., Westendorf, N., Marin, L., Iazbik, M. C., Vicario, F. C., Sanz, P., & Ruano, R.
- Affiliations
- The Ohio State University Comprehensive Cancer Center – Arthur G. James Cancer Hospital and Richard J. Solove Research Institute (US)
- IDEXX Laboratories (United States) (US)
- The Ohio State University (US)
- The Ohio State University
- Clínica Veterinaria Merced
- Scientific Institute for Veterinary Medicine of Serbia (RS)
- Clínica Mediterráneo
- Corresponding author
- Couto, C. Guillermo
- Venue
- Vector-Borne and Zoonotic Diseases
- Publisher
- SAGE Publications
- Published
- 2010-11
- Volume
- 10
- Issue
- 9
- Pages
- 885-888
- ISSN
- 1530-3667 (ISSN-L)
- 1557-7759
- OpenAlex
- W2007024121
- Cited by
- 31
- References
- 10
- Citations by year
- 2025: 1 · 2023: 1 · 2021: 3 · 2020: 3 · 2018: 3 · 2017: 1 · 2016: 2 · 2015: 2 · 2014: 2 · 2013: 4 · 2012: 6
- FWCI
- 3.19
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Vector-Borne and Zoonotic Diseases (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Immunology and Microbiology › Parasitology › Vector-borne infectious diseases
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Research on Leishmaniasis Studies
- Life Sciences › Immunology and Microbiology › Parasitology › Bartonella species infections research
- Keywords
- vector-borne diseases, shelter dogs, point-of-care assay, Ehrlichia canis, Leishmania infantum, Dirofilaria immitis, Borrelia burgdorferi, seroprevalence, co-infection, canine leishmaniasis, canine ehrlichiosis, canine anaplasmosis, animal shelters
- MeSH terms
- Animals, Antibodies, Bacterial, Antibodies, Helminth, Antibodies, Protozoan, Blood Cell Count, Dirofilaria immitis, Dirofilariasis, Dog Diseases, Dogs, Female, Gram-Positive Bacteria, Hematocrit, Leishmaniasis, Visceral, Male, Spain, Prevalence, Seroepidemiologic Studies, Gram-Negative Bacterial Infections, Leishmania infantum, Ehrlichia canis
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