Serological Reactivity to Ehrlichia canis, Anaplasma phagocytophilum, Neorickettsia risticii, Borrelia burgdorferi and Rickettsia conorii in Dogs from Northwestern Spain
Abstract
The aim of the present work was to investigate the seroprevalence against Ehrlichia canis (Ec), Anaplasma phagocytophilum (Ap), Neorickettsia risticii (Nr), Rickettsia conorii (Rc), and Borrelia burgdorferi (Bb) in two different clusters of canine samples from Northwestern Spain. Cluster 1 included 479 dogs presented at veterinary clinics located in Ourense and Pontevedra. Cluster II included 170 dogs from the public kennel of Ourense. All 649 canine serum samples were analyzed by immunofluorescent antibody test. Prevalences against the above-mentioned agents in cluster I were: Rc (24.6%), Bb (6.26%), Ec (3.13%), Ap (5.01%), and Nr (1.04%), whereas for cluster II were: Rc (50%), Bb (8.8%), Ec (54.7%), Ap (45.3%), and Nr (4.7%). Rc was significantly associated with age and history of exposure to ticks, and Bb showed a statistical relationship with age and clinical status. Ec and Ap were related to the occupation of the dogs, with stray dogs being the most frequently seropositive. Furthermore, seroreactivity against Ec and Ap was significantly higher in Ourense than in Pontevedra. The univariate analysis demonstrated a significant concomitant seroreactivity between Ec and Ap and between Rc and Ec and Ap antigens. The seroreactivity to Nr must be interpreted very cautiously as this infectious agent has been seldom reported outside North America.
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Other previous studies have published seroprevalence data for Anaplasma spp. and E. canis mostly from localized areas or provinces of Asturias, Aragon, Catalonia, Andalusia, the Valencian Community or the Balearic Islands.
“other previous studies have published seroprevalence data that were mostly reported in localized areas or provinces of the autonomous communities of Asturias, Aragon, Catalonia, Andalusia, the Valencian Community or the Balearic Islands (9, 23, 69)”
From Current Distribution of Selected Vector-Borne Diseases in Dogs in Spain (2020) · cites this paper as [69]
Cites
Infectious Diseases of the Dog and Cat
Greene, C. E. (1999) · Australian Veterinary Journal · doi:10.1111/j.1751-0813.1999.tb11241.x · Cited by 2,573 · see citing works
Breitschwerdt, E. B., Hegarty, B. C., & Hancock, S. I. (1998) · Journal of Clinical Microbiology · doi:10.1128/jcm.36.9.2645-2651.1998 · Cited by 289 · see citing works
Dumler, J. S., Asanovich, K. M., Bakken, J., et al. (1995) · Journal of Clinical Microbiology · doi:10.1128/jcm.33.5.1098-1103.1995 · Cited by 278 · see citing works
Amplification of Ehrlichial DNA from Dogs 34 Months after Infection with Ehrlichia canis
Harrus, S., Waner, T., Aizenberg, I., et al. (1998) · Journal of Clinical Microbiology · doi:10.1128/jcm.36.1.73-76.1998 · Cited by 194 · see citing works
Waner, T., Harrus, S., Jongejan, F., et al. (2001) · Veterinary Parasitology · doi:10.1016/s0304-4017(00)00407-6 · Cited by 180 · see citing works
Trotz‐William, L. A., & Trees, A. J. (2003) · Veterinary Record · doi:10.1136/vr.152.4.97 · Cited by 129 · see citing works
Dambach, D. M., Smith, C., Lewis, R. M., & Winkle, T. J. V. (1997) · Veterinary Pathology · doi:10.1177/030098589703400201 · Cited by 127 · see citing works
A serological study of exposure to arthropod-borne pathogens in dogs from northeastern Spain
Solano-Gallego, L., Llull, J., Osso, M., Hegarty, B., & Breitschwerdt, E. (2006) · Veterinary Research · doi:10.1051/vetres:2005054 · Cited by 122 · see citing works
Ixodes hexagonus is the main candidate as vector of Theileria annae in northwest Spain
Camacho, A. T., Pallas, E. P., Gestal, J. J., et al. (2003) · Veterinary Parasitology · doi:10.1016/s0304-4017(02)00417-x · Cited by 112 · see citing works
Nicholson, W. L., Comer, J. A., Sumner, J. W., et al. (1997) · Journal of Clinical Microbiology · doi:10.1128/jcm.35.6.1510-1516.1997 · Cited by 110 · see citing works
Suksawat, J., Xuejie, Y., Hancock, S. I., et al. (2001) · Journal of Veterinary Internal Medicine · doi:10.1892/0891-6640(2001)015 · Cited by 91 · see citing works
Levy, S. A., & Magnarelli, L. A. (1992) · Journal of the American Veterinary Medical Association · doi:10.2460/javma.1992.200.03.344 · Cited by 89 · see citing works
Renal lesions associated with Borrelia burgdorferi infection in a dog
Grauer, G. F., Burgess, E. C., Cooley, A. J., et al. (1988) · Journal of the American Veterinary Medical Association · doi:10.2460/javma.1988.193.02.237 · Cited by 77 · see citing works
Febrile Illness Associated withRickettsia conoriiInfection in Dogs from Sicily
Solano‐Gallego, L., Kidd, L. B., Trotta, M., Marco, M. D., Caldín, M., Furlanello, T., & Breitschwerdt, E. B. (2006) · Emerging infectious diseases · doi:10.3201/eid1212.060326 · Cited by 72 · see citing works
First isolation and molecular characterization of Ehrlichia canis in Spain
Aguirre, E., Sainz, Á., Dunner, S., et al. (2004) · Veterinary Parasitology · doi:10.1016/j.vetpar.2004.08.007 · Cited by 64 · see citing works
Self-citationShin, S. J., Chang, Y., Jacobson, R. H., Shaw, E. L., Lauderdale, T.-L. Y., Appel, M. J. G., & Lein, D. H. (1993) · Veterinary Microbiology · doi:10.1016/0378-1135(93)90137-v · Cited by 62 · see citing works
Lindenmayer, J. M., Weber, M., Bryant, J., et al. (1990) · Journal of Clinical Microbiology · doi:10.1128/jcm.28.1.92-96.1990 · Cited by 52 · see citing works
Anaplasma phagocytophila as an Abortifacient Agent in Sheep Farms from Northern Spain
García-Pérez, A. L., Barandika, J. F., Oporto, B., et al. (2003) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.2003.tb07406.x · Cited by 50 · see citing works
Genetic Characterization of Anaplasma (Ehrlichia) platys in Dogs in Spain
Aguirre, E., Tesouro, M. A., Ruiz, L. M., et al. (2006) · Journal of Veterinary Medicine Series B · doi:10.1111/j.1439-0450.2006.00937.x · Cited by 48 · see citing works
Self-citationTick species and tick-borne infections identified in population from a rural area of Spain
Merino-Fernández, F. J., Nebreda-Mayoral, T., SERRANO, J. L., et al. (2005) · Epidemiology and Infection · doi:10.1017/s0950268805004061 · Cited by 47 · see citing works
Equine Monocytic Ehrlichiosis (Potomac Horse Fever) in Horses in Uruguay and Southern Brazil
Dutra, F., Schuch, L. F. D., Delucchi, E., et al. (2001) · Journal of Veterinary Diagnostic Investigation · doi:10.1177/104063870101300514 · Cited by 44 · see citing works
Spotted Fever Group Rickettsiae in Ticks Feeding on Humans in Northwestern Spain
Fernández‐Soto, P., Pérez–Sánchez, R., Álamo-Sanz, R., et al. (2006) · Annals of the New York Academy of Sciences · doi:10.1196/annals.1374.063 · Cited by 43 · see citing works
Segura-Porta, F., Diestre-Ortin, G., Ortuño-Romero, A., et al. (1998) · European Journal of Epidemiology · doi:10.1023/a:1007479909654 · Cited by 41 · see citing works
Oteo, J. A., Gil, H., Barral, M., Pérez, A., Jiménez, S., Blanco, J. R., de Artola, V. M., García-Pérez, A. L., & Juste, R. A. (2001) · Epidemiology and Infection · doi:10.1017/s0950268801005878 · Cited by 37 · see citing works
Hovius, J. W., Hovius, K. E., Oei, A., Houwers, D. J., & van Dam, A. P. (2000) · Journal of Clinical Microbiology · doi:10.1128/jcm.38.7.2611-2621.2000 · Cited by 34 · see citing works
Epidemiological characteristics of dogs with Lyme borreliosis in the province of Soria (Spain)
Merino-Fernández, F. J., Serrano, J. L., Saz, J. V., Nebreda-Mayoral, T., Gegúndez, M. I., & Beltrán, M. (2000) · European Journal of Epidemiology · doi:10.1023/a:1007690807637 · Cited by 33 · see citing works
Serological Evaluation of Anaplasma phagocytophilum Infection in Livestock in Northwestern Spain
Amusategui, I., Sainz, Á., & Tesouro, M. A. (2006) · Annals of the New York Academy of Sciences · doi:10.1196/annals.1374.091 · Cited by 33 · see citing works
Self-citationWaner, T., Strenger, C., Keysary, A., & Harrus, S. (1998) · Veterinary Immunology and Immunopathology · doi:10.1016/s0165-2427(98)00198-6 · Cited by 31 · see citing works
Seroprevalence of canine ehrlichiosis in Castilla-León (north-west Spain)
Sainz, Á., Delgado, S., Amusategui, I., Tesouro, M. A., & Cármenes, P. (1996) · Preventive Veterinary Medicine · doi:10.1016/s0167-5877(96)01060-4 · Cited by 30 · see citing works
Self-citationOporto, B., Gil, H., Barral, M., et al. (2003) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.2003.tb07344.x · Cited by 28 · see citing works
Seroepidemiological survey forBorrelia burgdorferi (Lyme disease) in dogs from northwestern of Spain
Delgado, S., & Cármenes, P. (1995) · European Journal of Epidemiology · doi:10.1007/bf01719437 · Cited by 26 · see citing works
Prevalence and Sequence Analyses of Neorickettsia risticii
Chae, J., Kim, E.-H., Kim, M., et al. (2003) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.2003.tb07372.x · Cited by 26 · see citing works
Gauthier, D. T., & Mansfield, L. S. (1999) · Journal of Veterinary Diagnostic Investigation · doi:10.1177/104063879901100309 · Cited by 24 · see citing works
Espejo, E., Font-Creus, B., Alegre-Segura, M. D., et al. (1990) · PubMed · Cited by 23 · see citing works
Magnarelli, L. A., Levy, S. A., IJdo, J. W., et al. (2001) · Journal of Medical Microbiology · doi:10.1099/0022-1317-50-10-889 · Cited by 23 · see citing works
Screening of the Prevalence of Antibodies to Borrelia Burgdorferi in Madrid Province, Spain
Lledó, L., Gegúndez, M. I., Saz, J. V., et al. (2004) · European Journal of Epidemiology · doi:10.1023/b:ejep.0000027349.48337.cb · Cited by 19 · see citing works
Delgado, S., & Cármenes, P. (1995) · European Journal of Epidemiology · doi:10.1007/bf01719315 · Cited by 17 · see citing works
Herrero, C., Pelaz, C., Alvar, J., et al. (1992) · European Journal of Epidemiology · doi:10.1007/bf00146379 · Cited by 14 · see citing works
Sheets, J., Rossi, C. A., Kearney, B. J., & Moore, G. E. (2000) · Journal of the American Veterinary Medical Association · doi:10.2460/javma.2000.216.1418 · Cited by 12 · see citing works
GARCÍA, J. C., Núñez, M. J., CASTRO, B., Fraile, F. J., LÓPEZ, A., MELLA, M. C., Blanco, A. O., SIEIRA, C., LOUREIRO, E., Portillo, A., et al. (2006) · Annals of the New York Academy of Sciences · doi:10.1196/annals.1374.106 · Cited by 11 · see citing works
Persistence of Antibodies to Rickettsia conorii in Dogs
Tesouro, M. A., Bacellar, F., Sainz, Á., et al. (1998) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.1998.tb11092.x · Cited by 11 · see citing works
Self-citationFont, A., Closa, J. M., & Mascort, J. (1992) · Veterinary Record · doi:10.1136/vr.130.11.227 · Cited by 9 · see citing works
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Guideline for veterinary practitioners on canine ehrlichiosis and anaplasmosis in Europe
Sainz, Á., Roura, X., Miró, G., Estrada-Peña, A., Kohn, B., Harrus, S., & Solano-Gallego, L. (2015) · Parasites & Vectors · doi:10.1186/s13071-015-0649-0 · Cited by 369 · see citing works
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Miró, G., Montoya, A., Roura, X., Gálvez, R., & Sainz, A. (2013) · Parasites & Vectors · doi:10.1186/1756-3305-6-117 · Cited by 97 · see citing works
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Mrljak, V., Kuleš, J., Mihaljević, Ž., Torti, M., Gotić, J., Crnogaj, M., Živičnjak, T., Mayer, I., Šmit, I., Bhide, M., et al. (2017) · Vector-Borne and Zoonotic Diseases · doi:10.1089/vbz.2016.1990 · Cited by 57 · see citing works
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Díaz-Regañón, D., Roura, X., Suárez, M. L., León, M., & Sainz, Á. (2020) · Parasites & Vectors · doi:10.1186/s13071-020-04172-5 · Cited by 37 · see citing works
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- Authors
- Amusategui, I., Tesouro, M. A., Kakoma, I., & Sainz, Á.
- Affiliations
- Universidad Complutense de Madrid (ES)
- Universidad de León (ES)
- University of Illinois Urbana-Champaign (US)
- Venue
- Vector-Borne and Zoonotic Diseases
- Publisher
- SAGE Publications
- Published
- 2008-12
- Volume
- 8
- Issue
- 6
- Pages
- 797-804
- ISSN
- 1530-3667 (ISSN-L)
- 1557-7759
- OpenAlex
- W2027154940
- Cited by
- 52
- References
- 42
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 1 · 2023: 1 · 2021: 5 · 2020: 4 · 2019: 2 · 2018: 4 · 2017: 1 · 2016: 1 · 2015: 8 · 2014: 3 · 2013: 3 · 2012: 5
- FWCI
- 2.73
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Vector-Borne and Zoonotic Diseases (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Universidad Complutense de Madrid
- Topics
- Life Sciences › Immunology and Microbiology › Parasitology › Vector-borne infectious diseases
- Life Sciences › Immunology and Microbiology › Parasitology › Bartonella species infections research
- Health Sciences › Veterinary › Small Animals › Brucella: diagnosis, epidemiology, treatment
- Keywords
- canine ehrlichiosis, canine anaplasmosis, Neorickettsia risticii, Borrelia burgdorferi, Rickettsia conorii, seroprevalence, dogs, tick exposure, stray dogs, zoonotic pathogens, Ourense, Pontevedra, serological cross-reactivity
- MeSH terms
- Animals, Antibodies, Bacterial, Bacterial Infections, Dog Diseases, Dogs, Serologic Tests, Spain, Seroepidemiologic Studies, Rickettsia conorii, Borrelia burgdorferi, Ehrlichia canis, Anaplasma phagocytophilum, Neorickettsia risticii
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