CoChat
Sign Up
Cited by 129Hematopoietic Stem Cell TransplantationMesenchymal stem cell researchTelomeres, Telomerase, and Senescence

The DOI matches its Crossref record; 56 of the 56 references it cites were found in a registry or an index.

Impaired Hematopoietic Stem Cell Functioning After Serial Transplantation and During Normal Aging

Kamminga, Leonie M., van Os, Ronald, Ausema, Albertina, Noach, Estelle J. K., Weersing, Ellen, Dontje, Bert, Vellenga, Edo, de Haan, Gerald·2005·STEM CELLS
DOI 10.1634/stemcells.2004-0066
Open this paper in CoChat — free account

Abstract

Adult somatic stem cells possess extensive self-renewal capacity, as their primary role is to replenish aged and functionally impaired tissues. We have previously shown that the stem cell pool in short-lived DBA/2 (D2) mice is reduced during aging, in contrast to long-lived C57BL/6 (B6) mice. This suggests the existence of a genetically determined mitotic clock operating in stem cells, which possibly limits organismal aging. In the study reported here, unfractionated bone marrow (BM) cells or highly purified Lin(-)Sca-1(+)c-kit(+) (LSK) cells were serially transplanted in lethally irradiated D2 and B6 mice. In both strains, serial transplantation resulted in a substantial loss of stem cell activity. However, as we estimate that in B6 mice, the maximum number of population doublings of primitive cells is approximately 30, in D2 mice this is only approximately 20, resulting in a 1,000-fold difference in expansion potential, irrespective of whether whole bone marrow or purified hematopoietic stem cells (HSCs) were transplanted. Interestingly, recipients reconstituted with serially transplanted BM cells were able to accept a freshly isolated graft without any further conditioning. Finally, we show that whereas transplantation of BM cells into healthy, nonconditioned, young B6 recipients does not lead to engraftment, young BM cells do engraft and provide multilineage reconstitution in nonirradiated aged mice. Our data clearly establish the relevance of an intrinsic, genetically controlled program associated with impaired stem cell functioning during aging.

Overview

Integrity signals

Registry

Crossref · 10.1634/stemcells.2004-0066

Cites

56 of 56 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

Flagged first, then by citations

Cited by

Sorted by citations

See all 129

Related

See all

Metadata

Authors
Kamminga, L. M., van Os, R., Ausema, A., Noach, E. J. K., Weersing, E., Dontje, B., Vellenga, E., & de Haan, G.
Affiliations
  • University of Groningen (NL)
  • Department of Stem Cell Biology, University of Groningen, Groningen, The Netherlands
  • University Medical Center Groningen (NL)
ORCID
Venue
STEM CELLS
Publisher
Oxford University Press (OUP)
Published
2005-01
Volume
23
Issue
1
Pages
82-92
ISSN
  • 1066-5099 (ISSN-L)
  • 1549-4918
DOI
10.1634/stemcells.2004-0066
OpenAlex
W2003077095
Cited by
128
References
56
Citations by year
2025: 3 · 2024: 2 · 2023: 2 · 2022: 1 · 2021: 4 · 2020: 10 · 2019: 8 · 2018: 10 · 2017: 7 · 2016: 12 · 2015: 6 · 2014: 6 · 2013: 8 · 2012: 5
FWCI
5.32
Type
journal article
Language
en
Open access
closed
Licence
Locations
  • Stem Cells (publishedVersion)
  • PubMed (publishedVersion)
  • University of Groningen research database (University of Groningen / Centre for Information Technology) (submittedVersion)
Funders
  • Ministry of Earth Sciences
  • Ministry of Education, India
Topics
  • Health Sciences › Medicine › Hematology › Hematopoietic Stem Cell Transplantation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Mesenchymal stem cell research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Telomeres, Telomerase, and Senescence
Keywords
hematopoietic stem cells, aging, serial transplantation, stem cell self-renewal, bone marrow transplantation, LSK cells, DBA/2 mice, C57BL/6 mice, engraftment, multilineage reconstitution
MeSH terms
Animals, Cell Count, Evaluation Studies as Topic, Female, Hematopoietic Stem Cells, Male, Mice, Inbred C57BL, Mice, Inbred DBA, Time Factors, Cellular Senescence, Hematopoietic Stem Cell Transplantation, Mice

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review