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Molecular Signature of Quiescent Satellite Cells in Adult Skeletal Muscle
Abstract
Skeletal muscle satellite cells play key roles in postnatal muscle growth and regeneration. To study molecular regulation of satellite cells, we directly prepared satellite cells from 8- to 12-week-old C57BL/6 mice and performed genome-wide gene expression analysis. Compared with activated/cycling satellite cells, 507 genes were highly upregulated in quiescent satellite cells. These included negative regulators of cell cycle and myogenic inhibitors. Gene set enrichment analysis revealed that quiescent satellite cells preferentially express the genes involved in cell-cell adhesion, regulation of cell growth, formation of extracellular matrix, copper and iron homeostasis, and lipid transportation. Furthermore, reverse transcription-polymerase chain reaction on differentially expressed genes confirmed that calcitonin receptor (CTR) was exclusively expressed in dormant satellite cells but not in activated satellite cells. In addition, CTR mRNA is hardly detected in nonmyogenic cells. Therefore, we next examined the expression of CTR in vivo. CTR was specifically expressed on quiescent satellite cells, but the expression was not found on activated/proliferating satellite cells during muscle regeneration. CTR-positive cells reappeared at the rim of regenerating myofibers in later stages of muscle regeneration. Calcitonin stimulation delayed the activation of quiescent satellite cells. Our data provide roles of CTR in quiescent satellite cells and a solid scaffold to further dissect molecular regulation of satellite cells. Disclosure of potential conflicts of interest is found at the end of this article.
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“the signature reveals the downregulation of genes that are involved in DNA replication and cell cycle progression.”
From Molecular regulation of stem cell quiescence (2013) · cites this paper as [15]
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“Examples of genes that are downregulated in all three quiescent cell types (HSCs, MuSCs and HFSCs) include genes encoding cyclin A2, cyclin B1, cyclin E2 and survivin”
From Molecular regulation of stem cell quiescence (2013) · cites this paper as [15]
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“a comparison of gene expression profiles of different types of quiescent stem cells, including HSCs45, MuSCs15 and HFSCs43, reveals a gene signature that is common to these quiescent stem cells (TABLE 1).”
From Molecular regulation of stem cell quiescence (2013) · cites this paper as [15]
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Metadata
- Authors
- Fukada, S.-I., Uezumi, A., Ikemoto, M., Masuda, S., Segawa, M., Tanimura, N., Yamamoto, H., Miyagoe-Suzuki, Y., & Takeda, S.
- Affiliations
- National Center of Neurology and Psychiatry (JP)
- Department of Molecular Therapy, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Tokyo, Japan
- The University of Osaka (JP)
- Mizuho Research & Technologies, Ltd. (Japan) (JP)
- Venue
- Stem Cells
- Publisher
- Oxford University Press (OUP)
- Published
- 2007-06-28
- Volume
- 25
- Issue
- 10
- Pages
- 2448-2459
- ISSN
- 1066-5099 (ISSN-L)
- 1549-4918
- OpenAlex
- W2122795085
- Cited by
- 506
- References
- 45
- Citations by year
- 2026: 14 · 2025: 20 · 2024: 34 · 2023: 26 · 2022: 35 · 2021: 32 · 2020: 37 · 2019: 36 · 2018: 31 · 2017: 35 · 2016: 31 · 2015: 32 · 2014: 23 · 2013: 27 · 2012: 33
- FWCI
- 5.16
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model (vor)
- Locations
- Stem Cells (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Ministry of Education, Culture, Sports, Science and Technology
- Japan Society for the Promotion of Science (14657158)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Congenital heart defects research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Keywords
- satellite cells, calcitonin receptor, skeletal muscle regeneration, satellite cell activation, gene expression profiling, cell cycle regulation, cell-cell adhesion, extracellular matrix, lipid transport
- MeSH terms
- Animals, Calcitonin, Cell Differentiation, Cell Division, Female, Mice, Inbred C57BL, Muscle Proteins, Regeneration, RNA, Messenger, Biomarkers, Cell Adhesion Molecules, Receptors, Calcitonin, Myogenic Regulatory Factors, Muscle, Skeletal, Gene Expression Regulation, Developmental, Cell Cycle Proteins, Gene Expression Profiling, Muscle Development, Satellite Cells, Skeletal Muscle, Apoptosis Regulatory Proteins, Mice
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