The DOI matches its Crossref record; 24 of the 24 references it cites were found in a registry or an index.
Novel Cardiac Precursor-Like Cells from Human Menstrual Blood-Derived Mesenchymal Cells
Abstract
Stem cell therapy can help repair damaged heart tissue. Yet many of the suitable cells currently identified for human use are difficult to obtain and involve invasive procedures. In our search for novel stem cells with a higher cardiomyogenic potential than those available from bone marrow, we discovered that potent cardiac precursor-like cells can be harvested from human menstrual blood. This represents a new, noninvasive, and potent source of cardiac stem cell therapeutic material. We demonstrate that menstrual blood-derived mesenchymal cells (MMCs) began beating spontaneously after induction, exhibiting cardiomyocyte-specific action potentials. Cardiac troponin-I-positive cardiomyocytes accounted for 27%–32% of the MMCs in vitro. The MMCs proliferated, on average, 28 generations without affecting cardiomyogenic transdifferentiation ability, and expressed mRNA of GATA-4 before cardiomyogenic induction. Hypothesizing that the majority of cardiomyogenic cells in MMCs originated from detached uterine endometrial glands, we established monoclonal endometrial gland-derived mesenchymal cells (EMCs), 76%–97% of which transdifferentiated into cardiac cells in vitro. Both EMCs and MMCs were positive for CD29, CD105 and negative for CD34, CD45. EMCs engrafted onto a recipient's heart using a novel 3-dimensional EMC cell sheet manipulation transdifferentiated into cardiac tissue layer in vivo. Transplanted MMCs also significantly restored impaired cardiac function, decreasing the myocardial infarction (MI) area in the nude rat model, with tissue of MMC-derived cardiomyocytes observed in the MI area in vivo. Thus, MMCs appear to be a potential novel, easily accessible source of material for cardiac stem cell-based therapy. Disclosure of potential conflicts of interest is found at the end of this article.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Isolation of Putative Progenitor Endothelial Cells for Angiogenesis
Asahara, T., Murohara, T., Sullivan, A., Silver, M., van der Zee, R., Li, T., Witzenbichler, B., Schatteman, G., & Isner, J. M. (1997) · Science · doi:10.1126/science.275.5302.964 · Cited by 8,725
Bone marrow cells regenerate infarcted myocardium
Orlic, D., Kajstura, J., Chimenti, S., Jakoniuk, I., Anderson, S. M., Li, B., Pickel, J., McKay, R., Nadal-Ginard, B., Bodine, D. M., et al. (2001) · Nature · doi:10.1038/35070587 · Cited by 5,155
Kocher, A. A., Schuster, M. D., Szabolcs, M. J., Takuma, S., Burkhoff, D., Wang, J., Homma, S., Edwards, N. M., & Itescu, S. (2001) · Nature Medicine · doi:10.1038/86498 · Cited by 2,540
Mesenchymal stem cells reside in virtually all post-natal organs and tissues
Meirelles, L. D. S., Chagastelles, P. C., & Nardi, N. B. (2006) · Journal of Cell Science · doi:10.1242/jcs.02932 · Cited by 2,485
Cardiomyocytes can be generated from marrow stromal cells in vitro
Makino, S., Fukuda, K., Miyoshi, S., Konishi, F., Kodama, H., Pan, J., Sano, M., Takahashi, T., Hori, S., Abe, H., et al. (1999) · Journal of Clinical Investigation · doi:10.1172/jci5298 · Cited by 1,884
Self-citationGnecchi, M., He, H., Liang, O. D., Melo, L. G., Morello, F., Mu, H., Noiseux, N., Zhang, L., Pratt, R. E., Ingwall, J. S., et al. (2005) · Nature Medicine · doi:10.1038/nm0405-367 · Cited by 1,677
Chen, S.-L., Fang, W.-W., Ye, F., Liu, Y.-H., Qian, J., Shan, S.-J., Zhang, J.-J., Chunhua, R. Z., Liao, L.-M., Lin, S., et al. (2004) · The American Journal of Cardiology · doi:10.1016/j.amjcard.2004.03.034 · Cited by 1,191
Shake, J. G., Gruber, P. J., Baumgartner, W. A., Senechal, G., Meyers, J., Redmond, J. M., Pittenger, M. F., & Martin, B. J. (2002) · The Annals of Thoracic Surgery · doi:10.1016/s0003-4975(02)03517-8 · Cited by 901
Endometrial regenerative cells: A novel stem cell population
Meng, X., Ichim, T. E., Zhong, J., Rogers, A., Yin, Z., Jackson, J., Wang, H., Ge, W., Bogin, V., Chan, K. W., et al. (2007) · Journal of Translational Medicine · doi:10.1186/1479-5876-5-57 · Cited by 630
Schwab, K. E., & Gargett, C. E. (2007) · Human Reproduction · doi:10.1093/humrep/dem265 · Cited by 545
Cited by
Ding, D.-C., Shyu, W.-C., & Lin, S.-Z. (2011) · Cell Transplantation · doi:10.3727/096368910x · Cited by 894
Endometrial stem/progenitor cells: the first 10 years
Gargett, C. E., Schwab, K. E., & Deane, J. A. (2015) · Human Reproduction Update · doi:10.1093/humupd/dmv051 · Cited by 561
Adult stem cells in the endometrium
Gargett, C. E., & Masuda, H. (2010) · Molecular Human Reproduction · doi:10.1093/molehr/gaq061 · Cited by 361
A novel marker of human endometrial mesenchymal stem-like cells
Masuda, H., Anwar, S. S., Bühring, H.-J., Rao, J. R., & Gargett, C. E. (2012) · Cell Transplantation · doi:10.3727/096368911x637362 · Cited by 294
Stem cell theory for the pathogenesis of endometriosis
Maruyama, T. (2012) · Frontiers in Bioscience · doi:10.2741/e589 · Cited by 109
Metadata
- Authors
- Hida, N., Nishiyama, N., Miyoshi, S., Kira, S., Segawa, K., Uyama, T., Mori, T., Miyado, K., Ikegami, Y., Cui, C., Kiyono, T., Kyo, S., Shimizu, T., Okano, T., Sakamoto, M., Ogawa, S., & Umezawa, A.
- Venue
- Stem Cells
- Publisher
- Oxford University Press (OUP)
- Published
- 2008-04-17
- Volume
- 26
- Issue
- 7
- Pages
- 1695-1704
- ISSN
- 1066-5099 (ISSN-L)
- 1549-4918
- OpenAlex
- W2002963491
- Cited by
- 342
- References
- 24
- FWCI
- 7.75
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Novel Cardiac Precursor-Like Cells from Human Menstrual Blood-Derived Mesenchymal Cells. https://app.cochat.ai/papers/10.1634/stemcells.2007-0826
- The paper
- Hida, N., Nishiyama, N., Miyoshi, S., Kira, S., Segawa, K., Uyama, T., Mori, T., Miyado, K., Ikegami, Y., Cui, C., Kiyono, T., Kyo, S., Shimizu, T., Okano, T., Sakamoto, M., Ogawa, S., & Umezawa, A. (2008). Novel Cardiac Precursor-Like Cells from Human Menstrual Blood-Derived Mesenchymal Cells. Stem Cells. https://doi.org/10.1634/stemcells.2007-0826
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
