Ecdysterone Accelerates Healing of Radiation-Induced Oral Mucositis in Rats by Increasing Matrix Cell Proliferation
Abstract
Development of oral mucositis represents a rate-limiting factor in radiation therapy for the treatment of head and neck, as well as other cancers. In this work, we investigated the treatment effect of ecdysterone (a steroid derived from the dry root of Achyranthes bidentate) on radiation-induced oral mucositis, and examined possible underlying mechanisms. Male Sprague-Dawley rats were exposed to 20 Gy X-ray irradiation (single dose, cranial localization) to induce oral mucositis. Possible therapeutic effects of ecdysterone on radiation-induced oral mucositis were investigated by monitoring weights, direct observations, visual scoring method and evaluation of hematoxylin and eosin staining. Assessments of leukocyte common antigen and proliferating cell nuclear antigen staining were also performed in the damaged areas of tongues harvested after irradiation, and changes in signaling pathways were investigated using Western blotting. The development and progression of radiation-induced oral mucositis in this model was similar to that observed in clinic patients. Ecdysterone effectively improved radiation-induced oral mucositis as assessed by direct observation and histopathology, and also increased proliferation of matrix cells, since the Ras-Raf-ERK signal pathway was found to be activated by its use. It was concluded that orally administered ecdysterone accelerated the healing process in a rat model of radiation-induced oral mucositis by upregulating the Ras-Raf-ERK signal pathway.
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Ecdysterone improved healing of the mucosa in radiation-induced oral mucositis by augmenting Ras-Raf-Erk pathway activity and increasing proliferation of matrix cells.
“Ecdysterone improved healing of the mucosa by augmenting activity of the Ras-Raf-Erk pathway and increasing the proliferation of matrix cells [61].”
From Molecular Targets of 20-Hydroxyecdysone in Mammals, Mechanism of Action: Is It a Calorie Restriction Mimetic and Anti-Aging Compound? (2025) · cites this paper as [61]
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“Ecdysterone improved healing of the mucosa by augmenting activity of the Ras-Raf-Erk pathway and increasing the proliferation of matrix cells [61].”
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Ecdysterone Attenuates the Development of Radiation-Induced Oral Mucositis in Rats at Early Stage
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Self-citation
- Authors
- Li, Y., Ailing, H., & Jian, P.
- Affiliations
- Hefei University of Technology (CN)
- Hefei University of Technology, Anhui Hefei, China
- ORCID
- Li, Yang
- Venue
- Radiation Research
- Publisher
- Radiation Research Society
- Published
- 2019-01-18
- Volume
- 191
- Issue
- 3
- Pages
- 237
- ISSN
- 0033-7587 (ISSN-L)
- OpenAlex
- W2913697074
- Cited by
- 5
- References
- 28
- Citations by year
- 2025: 1 · 2021: 1 · 2020: 2 · 2019: 1
- FWCI
- 0.74
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Radiation Research (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Anhui University
- Anhui Medical University
- U.S. Food and Drug Administration
- Topics
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Oral health in cancer treatment
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Effects of Radiation Exposure
- Health Sciences › Dentistry › Periodontics › Oral Health Pathology and Treatment
- Keywords
- 20-hydroxyecdysone, oral mucositis, radiation-induced mucositis, radiotherapy complications, head and neck cancer, wound healing, Achyranthes bidentata, CD45, proliferating cell nuclear antigen
- MeSH terms
- Animals, Ecdysterone, Male, Radiation Dosage, Radiation Injuries, Experimental, Stomatitis, Signal Transduction, Up-Regulation, Rats, Sprague-Dawley, ras Proteins, Extracellular Signal-Regulated MAP Kinases, raf Kinases, Cell Proliferation, Rats
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