Therapeutic Effect of Ecdysterone Combine Paeonol Oral Cavity Direct Administered on Radiation-Induced Oral Mucositis in Rats
Open accessAbstract
Radiation-induced oral mucositis represents an influential factor in cancer patients’ accepted radiation therapy, especially in head and neck cancer. This research investigates the treatment effect of Ecdysterone (a steroid derived from the dry root of Achyranthes bidentate) and Paeonol (a compound derived from Cortex Moutan) on radiation-induced oral mucositis and possible underlying mechanisms. Concisely, 20 Gy of X-rays (single-dose) irradiated the cranial localization in rats for the modeling of oral mucositis. The therapeutic effects of Ecdysterone-Paeonol oral cavity directly administered on radiation-induced oral mucositis were investigated by weight changes, direct observations, visual scoring methods, ulcer area/total area, and basic recovery days. Assessments of tumor necrosis factor α and interleukin-6 were performed to evaluate the inflammatory cytokines secretion in the damaged areas of tongues harvested post-treatment, and changes in signaling pathways were investigated by Western blotting. System Drug Target (SysDT) methods revealed the targets of Ecdysterone-Paeonol in order to support compound-target network construction. Four representative targets with different functions were chosen. The binding interactions between the compound and receptor were evaluated by molecular docking to investigate the binding affinity of the ligand to their protein targets. Ecdysterone-Paeonol, administered orally, effectively improved radiation-induced oral mucositis in rats, and the therapeutic effect was better than Ecdysterone administered orally on its own. In this study, calculational chemistry revealed that Ecdysterone-Paeonol affected 19 function targets associated with radiation-induced oral mucositis, including apoptosis, proliferation, inflammation, and wound healing. These findings position Ecdysterone-Paeonol as a potential treatment candidate for oral mucositis acting on multiple targets in the clinic.
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Calculational chemistry showed that ecdysterone-paeonol may interact with 19 targets functional in radiation-induced oral mucositis, including apoptosis, inflammation, proliferation, and wound healing.
“calculational chemistry showed that ecdysterone-paeonol may interact with 19 targets that are functional in RIOM, including apoptosis, inflammation, proliferation, and wound healing [62].”
From Molecular Targets of 20-Hydroxyecdysone in Mammals, Mechanism of Action: Is It a Calorie Restriction Mimetic and Anti-Aging Compound? (2025) · cites this paper as [62]
20E combined with paeonol was more ameliorating in radiation-induced oral mucositis.
“20E was even more ameliorating in combination with paeonol”
From Molecular Targets of 20-Hydroxyecdysone in Mammals, Mechanism of Action: Is It a Calorie Restriction Mimetic and Anti-Aging Compound? (2025) · cites this paper as [62]
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Cites
Cytoscape: A software Environment for integrated models of biomolecular interaction networks
Shannon, P., Markiel, A., Ozier, O., Baliga, N. S., Wang, J. T., Ramage, D., Amin, N., Schwikowski, B., & Ideker, T. (2003) · Genome Research · doi:10.1101/gr.1239303 · Cited by 51,888 · see citing works
Chamberlain, G., Fox, J. M., Ashton, B. A., & Middleton, J. (2007) · Stem Cells · doi:10.1634/stemcells.2007-0197 · Cited by 2,376 · see citing works
Liang, H., Liang, P., Xu, Y., Wu, J., Liang, T., & Xu, X. (2009) · Journal of Neurotrauma · doi:10.1089/neu.2008.0850 · Cited by 1,743 · see citing works
Using AutoDock for Ligand‐Receptor Docking
Morris, G. M., Huey, R., & Olson, A. J. (2008) · Current Protocols in Bioinformatics · doi:10.1002/0471250953.bi0814s24 · Cited by 1,312 · see citing works
Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium
Gaudet, P., Livstone, M. S., Lewis, S., & Thomas, P. D. (2011) · Briefings in Bioinformatics · doi:10.1093/bib/bbr042 · Cited by 901 · see citing works
Elting, L. S., Cooksley, C. D., Chambers, M. S., & Garden, A. S. (2007) · International Journal of Radiation Oncology*Biology*Physics · doi:10.1016/j.ijrobp.2007.01.053 · Cited by 535 · see citing works
Mucositis: The impact, biology and therapeutic opportunities of oral mucositis
Sonis, S. T. (2009) · Oral Oncology · doi:10.1016/j.oraloncology.2009.08.006 · Cited by 525 · see citing works
Radiation-Induced Oral Mucositis
Maria, O. M., Eliopoulos, N., & Muanza, T. M. (2017) · Frontiers in Oncology · doi:10.3389/fonc.2017.00089 · Cited by 467 · see citing works
Yu, H., Chen, M., Xu, X. J., Li, Y., Zhao, H., Fang, Y., Li, X., Zhou, W., Wang, W., & Wang, Y. (2012) · PLoS ONE · doi:10.1371/journal.pone.0037608 · Cited by 422 · see citing works
Oral Mucositis Complicating Chemotherapy and/or Radiotherapy: Options for Prevention and Treatment
Köstler, W. J., Hejna, M., Wenzel, C.-K., & Zielinski, C. (2001) · CA A Cancer Journal for Clinicians · doi:10.3322/canjclin.51.5.290 · Cited by 364 · see citing works
He, D., & Dai, S. (2011) · Frontiers in Pharmacology · doi:10.3389/fphar.2011.00010 · Cited by 350 · see citing works
Pathobiology of oral mucositis: novel insights and opportunities.
Sonis, S. T. (2007) · PubMed · Cited by 302 · see citing works
Scully, C., Sonis, S. T., & Dios, P. D. (2006) · Oral Diseases · doi:10.1111/j.1601-0825.2006.01258.x · Cited by 253 · see citing works
Qu, L.-K., Huang, S. H., Baltzis, D., Rivas-Estilla, A.-M., Pluquet, O., Hatzoglou, M., Koumenis, C., Taya, Y., Yoshimura, A., & Koromilas, A. E. (2004) · Genes & Development · doi:10.1101/gad.1165804 · Cited by 247 · see citing works
Molecular Docking of Intercalators and Groove-Binders to Nucleic Acids Using Autodock and Surflex
Holt, P. A., Chaires, J. B., & Trent, J. O. (2008) · Journal of Chemical Information and Modeling · doi:10.1021/ci800063v · Cited by 226 · see citing works
Anti‐inflammatory and analgesic effects of paeonol in carrageenan‐evoked thermal hyperalgesia
Chou, T. (2003) · British Journal of Pharmacology · doi:10.1038/sj.bjp.0705360 · Cited by 210 · see citing works
Oral Mucositis Induced By Anticancer Therapies
Al‐Ansari, S., Zecha, J. A. E. M., Barasch, A., de Lange, J., Rozema, F. R., & Raber‐Durlacher, J. E. (2015) · Current Oral Health Reports · doi:10.1007/s40496-015-0069-4 · Cited by 198 · see citing works
Proteolysis of Human Thrombin Generates Novel Host Defense Peptides
Papareddy, P., Rydengård, V., Pasupuleti, M., Walse, B., Mörgelin, M., Chalupka, A., Malmsten, M., & Schmidtchen, A. (2010) · PLoS Pathogens · doi:10.1371/journal.ppat.1000857 · Cited by 167 · see citing works
Paeonol inhibits anaphylactic reaction by regulating histamine and TNF-α
Kim, S. H., Kim, S., Park, M., Kim, S., Park, Y., Ho-Jeong, N. A., Kim, H., Shin, M.-K., & Ahn, K.-S. (2004) · International Immunopharmacology · doi:10.1016/j.intimp.2003.12.013 · Cited by 119 · see citing works
Yang, Y., Li, Y., Wang, J., Sun, K., Tao, W., Wang, Z., Xiao, W., Pan, Y., Zhang, S., & Wang, Y. (2017) · ACS Chemical Biology · doi:10.1021/acschembio.6b00762 · Cited by 90 · see citing works
Paeonol Attenuates High-Fat-Diet-Induced Atherosclerosis in Rabbits by Anti-Inflammatory Activity
Li, H., Dai, M., & Jia, W. P. (2008) · Planta Medica · doi:10.1055/s-0028-1088332 · Cited by 85 · see citing works
Synthetic peptides bind to high-affinity thrombin receptors and modulate thrombin mitogenesis.
Glenn, K. C., Frost, G. H., Bergmann, J. S., & Carney, D. H. (1988) · PubMed · Cited by 81 · see citing works
Anti-Inflammatory and Anticoagulative Effects of Paeonol on LPS-Induced Acute Lung Injury in Rats
Fu, P., Wu, C., Tsai, T., & Hsieh, C. (2012) · Evidence-based Complementary and Alternative Medicine · doi:10.1155/2012/837513 · Cited by 68 · see citing works
Paeonol alleviated acute alcohol-induced liver injury via SIRT1/Nrf2/NF-κB signaling pathway
Sun, X. B., Wang, P., Yao, L., Wang, W., Gao, Y., Zhang, J., & Fu, Y. (2018) · Environmental Toxicology and Pharmacology · doi:10.1016/j.etap.2018.04.016 · Cited by 62 · see citing works
Dental Management of Patients Who Have Undergone Oral Cancer Therapy
Villa, A., & Akintoye, S. O. (2017) · Dental Clinics of North America · doi:10.1016/j.cden.2017.08.010 · Cited by 55 · see citing works
Spalinger, M. R., Lang, S., Vavricka, S. R., Fried, M., Rogler, G., & Scharl, M. M. (2013) · PLoS ONE · doi:10.1371/journal.pone.0072384 · Cited by 50 · see citing works
Transdermal delivery of paeonol using cubic gel and microemulsion gel
Shen, Q., Luo, & Chen, C. (2011) · International Journal of Nanomedicine · doi:10.2147/ijn.s22667 · Cited by 44 · see citing works
Li, C., Yang, L., Wu, H., & Dai, M. (2018) · Frontiers in Pharmacology · doi:10.3389/fphar.2018.00095 · Cited by 44 · see citing works
Self-citationWang, J., Li, Y., Yang, Y., Du, J., Zhao, M., Lin, F., Zhang, S., & Wang, B. (2017) · Molecular Pharmaceutics · doi:10.1021/acs.molpharmaceut.7b00505 · Cited by 38 · see citing works
Wang, J., Li, Y., Yang, Y., Chen, X., Du, J., Zheng, Q., Liang, Z., & Wang, Y. (2017) · Scientific Reports · doi:10.1038/s41598-017-01613-7 · Cited by 33 · see citing works
Wang, J., Yang, Y., Li, Y., & Wang, Y. (2016) · Journal of Agricultural and Food Chemistry · doi:10.1021/acs.jafc.6b01067 · Cited by 24 · see citing works
Mucositis guidelines: what have they achieved, and where to from here?
Keefe, D. M. K. (2006) · Supportive Care in Cancer · doi:10.1007/s00520-006-0056-3 · Cited by 22 · see citing works
Wang, Q., Xu, X., Kang, Z., Zhang, Z., & Li, Y. (2019) · Artificial Cells Nanomedicine and Biotechnology · doi:10.1080/21691401.2019.1613418 · Cited by 21 · see citing works
Zhang, H., & D'Souza, W. D. (2018) · Journal of Medical Physics · doi:10.4103/jmp.jmp_78_17 · Cited by 9 · see citing works
Li, Y., Ailing, H., & Jian, P. (2019) · Radiation Research · doi:10.1667/rr15171.1 · Cited by 5 · see citing works
Self-citationYang, Y., Li, Y., Zhou, W., Chen, Y., Wu, Q., Pan, Y., Zhang, S., & Yang, L. (2018) · Journal of Biomolecular Structure and Dynamics · doi:10.1080/07391102.2018.1517612 · Cited by 4 · see citing works
Self-citation
Flagged first, then by citations
1 of the 37 references couldn’t be matched to a known paper.
Cited by
Sorted by citations
Radiotherapy-Induced Digestive Injury: Diagnosis, Treatment and Mechanisms
Chen, G., Han, Y., Zhang, H., Tu, W., & Zhang, S. (2021) · Frontiers in Oncology · doi:10.3389/fonc.2021.757973 · Cited by 56 · see citing works
Paeonol alleviates primary dysmenorrhea in mice via activating CB2R in the uterus
Peng, Y., Zheng, X., Fan, Z., Zhou, H., Zhu, X., Wang, G., & Liu, Z. (2019) · Phytomedicine · doi:10.1016/j.phymed.2019.153151 · Cited by 36 · see citing works
Ecdysterone and Turkesterone—Compounds with Prominent Potential in Sport and Healthy Nutrition
Todorova, V., Ivanova, S., Chakarov, D., Kraev, K., & Ivanov, K. (2024) · Nutrients · doi:10.3390/nu16091382 · Cited by 31 · see citing works
“[97]”
Qi-Shun, G. E. N. G., Rui-Juan, L. I. U., Zhi-Bo, S. H. E. N., Qian, W. E. I., ZHENG, Y.-Y., Lan-Qi, J. I. A., Long-Hao, W. A. N. G., Li-Feng, L. I., Jun, L. I., & Wen-Hua, X. U. E. (2021) · Chinese Journal of Natural Medicines · doi:10.1016/s1875-5364(22)60150-x · Cited by 21 · see citing works
Anti-Adipogenic Activity of Rhaponticum carthamoides and Its Secondary Metabolites
Todorova, V., Savova, M. S., Ivanova, S., Ivanov, K., & Georgiev, M. I. (2023) · Nutrients · doi:10.3390/nu15133061 · Cited by 12 · see citing works
β-ecdysone promotes osteogenic differentiation of bone marrow mesenchymal stem cells
You, W., & Xu, Z. (2020) · The Journal of Gene Medicine · doi:10.1002/jgm.3207 · Cited by 11 · see citing works
Investigation of paeonol in dermatological diseases: an animal study review
Ju, J., Song, T., Shi, J., & Li, J. (2024) · Frontiers in Pharmacology · doi:10.3389/fphar.2024.1450816 · Cited by 12 · see citing works
Zádor, E. (2025) · Cells · doi:10.3390/cells14060431 · Cited by 9 · see citing works
“, and calculational chemistry showed that ecdysterone-paeonol may interact with 19 targets that are functional in RIOM, including apoptosis, inflammation, proliferation, and wound healing [62].”
Pan, M., Wang, Q., Liu, Y., Xiao, N., Niu, X., Wu, D., Wang, T., Yan, G., & Shao, J. (2022) · Medical Mycology · doi:10.1093/mmy/myac011 · Cited by 10 · see citing works
Gao, Z., Guan, L., Liu, Z., Yan, F., Fang, S., Zhang, X., & Gao, C. (2022) · Annals of Translational Medicine · doi:10.21037/atm-22-767 · Cited by 7 · see citing works
Dobrowolska, E., Motyka, S., Szopa, A., & Ekiert, H. (2022) · Farmacja Polska · doi:10.32383/farmpol/146835 · Cited by 1 · see citing works
Pharmacokinetic and Safety Profiles of Phytoecdysteroids in Mammals
Dinan, L. N., & Lafont, R. (2026) · Springer eBooks · doi:10.1007/978-981-95-8423-9_7
- Authors
- Yang, L., & Pan, J.
- Affiliations
- School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, China
- Hefei University of Technology (CN)
- Corresponding author
- Pan, Jian
- Venue
- International Journal of Molecular Sciences
- Publisher
- MDPI AG
- Published
- 2019-08-03
- Volume
- 20
- Issue
- 15
- Pages
- 3800
- ISSN
- 1422-0067 (ISSN-L)
- PMC
- PMC6695810
- OpenAlex
- W2966252695
- Cited by
- 12
- References
- 37
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 2 · 2023: 1 · 2022: 3 · 2021: 2 · 2020: 1 · 2019: 1
- FWCI
- 1.29
- Type
- journal article
- Language
- en
- Open access
- gold
- dx.doi.org
- Licence
- Locations
- International Journal of Molecular Sciences (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Topics
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Oral health in cancer treatment
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Effects of Radiation Exposure
- Health Sciences › Dentistry › Periodontics › Oral Health Pathology and Treatment
- Keywords
- radiation-induced oral mucositis, 20-hydroxyecdysone, paeonol, Achyranthes bidentata, Moutan Cortex, head and neck radiotherapy, TNF-α, interleukin-6, inflammatory cytokines, apoptosis, wound healing, compound-target network, molecular docking, radiosensitization
- MeSH terms
- Abnormalities, Radiation-Induced, Acetophenones, Animals, Disease Models, Animal, Drug Combinations, Ecdysterone, Humans, Mouth, Radiation Injuries, Stomatitis, Signal Transduction, Apoptosis, Rats, Molecular Docking Simulation
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