Cited by 19Stress Responses and CortisolNeuropeptides and Animal PhysiologyNeuroendocrine regulation and behavior

Congenital vasopressin deficiency and acute and chronic opiate effects on hypothalamo-pituitary-adrenal axis activity in Brattleboro rats

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Domokos, Ágnes, Mergl, Zsuzsa, Barna, István, Makara, Gábor B, Zelena, Dóra·2007·Journal of Endocrinology

Abstract

A growing body of evidence suggests that vasopressinergic activity in the hypothalamus is important in stress-related behaviors (like drug abuse) in line with a role in the regulation of the hypothalamo-pituitary–adrenal axis (HPA). We hypothesized that in the naturally vasopressin-deficient Brattleboro rat, acute and chronic morphine treatment may lead to reduced HPA axis activity. Rats were treated either with a single dose of morphine (10 mg/kg subcutaneously) and serial blood samples were taken or were treated twice daily with increasing doses of morphine (10–100 mg/kg subcutaneously) for 16 days and animals were killed by decapitation 4 or 16 h after the last injection. Single morphine injection induced a biphasic ACTH and corticosterone elevation with smaller increases in vasopressin-deficient rats. Chronic morphine treatment induced the typical somatic and HPA axis changes of chronic stress; the absence of vasopressin did not prevent these changes. In rats repeatedly treated with morphine plasma, ACTH and corticosterone levels were elevated both 4 and 16 h after the last injection (short and long withdrawal) and the absence of vasopressin attenuated this response. Our data suggest that vasopressin plays a prominent role in morphine treatment and withdrawal-induced acute hormonal changes, but does not affect development of chronic hyperactivity of the HPA axis.

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Authors
Domokos, Á., Mergl, Z., Barna, I., Makara, G. B., & Zelena, D.
Affiliations
HUN-REN Institute of Experimental Medicine (HU)
Venue
Journal of Endocrinology
Published
2007-10-16
Volume
196
Issue
1
Pages
113-121
ISSN
  • 0022-0795 (ISSN-L)
  • 1479-6805
OpenAlex
W2104838175
Cited by
19
References
35
Citations by year
2023: 1 · 2021: 2 · 2020: 1 · 2015: 1 · 2013: 3 · 2012: 2
FWCI
1.16
Type
journal article
Language
en
Open access
Licence
public-domain
Locations
  • Journal of Endocrinology (publishedVersion, public-domain)
  • PubMed (publishedVersion)
Funders
Hungarian Scientific Research Fund (T 043161)
Topics
  • Life Sciences › Neuroscience › Behavioral Neuroscience › Stress Responses and Cortisol
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
  • Social Sciences › Psychology › Social Psychology › Neuroendocrine regulation and behavior
Keywords
vasopressin deficiency, Brattleboro rats, morphine, opioid withdrawal, hypothalamic-pituitary-adrenal axis, ACTH, corticosterone, chronic stress, stress response, drug abuse
MeSH terms
Adrenal Glands, Adrenocorticotropic Hormone, Animals, Arginine Vasopressin, Corticosterone, Corticotropin-Releasing Hormone, Hypothalamus, Male, Morphine, Mutation, Pituitary Gland, Pro-Opiomelanocortin, Rats, Brattleboro, RNA, Messenger, In Situ Hybridization, Rats

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