Cited by 185Bone Metabolism and DiseasesBone health and osteoporosis researchBone and Dental Protein Studies

Osteoprotegerin mRNA is expressed in primary human osteoblast-like cells: down-regulation by glucocorticoids.

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Vidal, NO, Brandstrom, H, Jonsson, KB, Ohlsson, C·1998·Journal of Endocrinology

Abstract

Osteoprotegerin (OPG) is a recently cloned member of the tumour necrosis factor receptor family. It has been suggested that this secreted glycoprotein acts as an inhibitor of osteoclastic differentiation. Expression of OPG has previously been demonstrated in a number of tissues. However, it is still unclear whether or not OPG is expressed by human osteoblasts. We have used the RNase protection assay to demonstrate the OPG transcript in primary cultured human osteoblast-like cells, human marrow stroma cells and osteosarcoma cell lines. Furthermore, we have studied the effect of glucocorticoids on OPG mRNA levels in these cells. We demonstrate that glucocorticoids decrease the OPG transcript in a dose- and time-dependent manner. The time-course study reveals that hydrocortisone (10(-6) M) decreases OPG mRNA levels within 2 h. This decrease is transient, reaching control levels again after 24 h. Our findings demonstrate that human osteoblasts express the mRNA corresponding to OPG, an inhibitor of osteoclast differentiation. The finding that OPG mRNA levels are decreased by glucocorticoids indicates that a reduced production of OPG from osteoblasts and/or marrow stroma cells could, in part, explain glucocorticoid-induced bone resorption.

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  1. Canalis E 1996 Mechanisms of glucocorticoid action in bone: implications to glucocorticoid-induced osteoporosis. Journal of Endocrinology and Metabolism 81 3441-3447.

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Authors
Vidal, N., Brandstrom, H., Jonsson, K., & Ohlsson, C.
Affiliations
  • Sahlgrenska University Hospital (SE)
  • Uppsala University (SE)
Venue
Journal of Endocrinology
Published
1998-10-01
Volume
159
Issue
1
Pages
191-195
ISSN
  • 0022-0795 (ISSN-L)
  • 1479-6805
OpenAlex
W2163584089
Cited by
185
References
28
Citations by year
2026: 1 · 2025: 1 · 2024: 2 · 2023: 3 · 2022: 5 · 2021: 1 · 2020: 1 · 2019: 1 · 2018: 3 · 2017: 1 · 2016: 5 · 2014: 3 · 2013: 2 · 2012: 11
FWCI
3.72
Type
journal article
Language
en
Open access
Locations
  • Journal of Endocrinology (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Novo Nordisk
  • Vetenskapsrådet
  • Novo Nordisk Fonden
  • Medical Research Council
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
  • Health Sciences › Medicine › Orthopedics and Sports Medicine › Bone health and osteoporosis research
  • Health Sciences › Medicine › Rheumatology › Bone and Dental Protein Studies
Keywords
osteoprotegerin, osteoblasts, osteoblast-like cells, glucocorticoids, cortisol, mRNA expression, osteoclast differentiation inhibition, bone resorption, mesenchymal stem cells, osteosarcoma cell lines
MeSH terms
Anti-Inflammatory Agents, Cells, Cultured, Densitometry, Dose-Response Relationship, Drug, Estradiol, Gene Expression Regulation, Genetic Techniques, Glycoproteins, Humans, Hydrocortisone, Osteoblasts, Stimulation, Chemical, Testosterone, Time Factors, Tumor Cells, Cultured, Receptors, Tumor Necrosis Factor, Receptors, Cytoplasmic and Nuclear, Osteoprotegerin

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