Osteoprotegerin mRNA is expressed in primary human osteoblast-like cells: down-regulation by glucocorticoids.
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Osteoprotegerin (OPG) is a recently cloned member of the tumour necrosis factor receptor family. It has been suggested that this secreted glycoprotein acts as an inhibitor of osteoclastic differentiation. Expression of OPG has previously been demonstrated in a number of tissues. However, it is still unclear whether or not OPG is expressed by human osteoblasts. We have used the RNase protection assay to demonstrate the OPG transcript in primary cultured human osteoblast-like cells, human marrow stroma cells and osteosarcoma cell lines. Furthermore, we have studied the effect of glucocorticoids on OPG mRNA levels in these cells. We demonstrate that glucocorticoids decrease the OPG transcript in a dose- and time-dependent manner. The time-course study reveals that hydrocortisone (10(-6) M) decreases OPG mRNA levels within 2 h. This decrease is transient, reaching control levels again after 24 h. Our findings demonstrate that human osteoblasts express the mRNA corresponding to OPG, an inhibitor of osteoclast differentiation. The finding that OPG mRNA levels are decreased by glucocorticoids indicates that a reduced production of OPG from osteoblasts and/or marrow stroma cells could, in part, explain glucocorticoid-induced bone resorption.
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Glucocorticoids suppress OPG mRNA expression in primary human osteoblasts and osteoblast cell lines while up-regulating RANKL expression.
“Glucocorticoids have been shown to suppress OPG mRNA expression in primary human osteoblasts and in osteoblast cell lines (254, 258), while up-regulating RANKL expression (254).”
From Receptor Activator of Nuclear Factor κB Ligand and Osteoprotegerin Regulation of Bone Remodeling in Health and Disease (2008) · cites this paper as [258]
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- Authors
- Vidal, N., Brandstrom, H., Jonsson, K., & Ohlsson, C.
- Affiliations
- Sahlgrenska University Hospital (SE)
- Uppsala University (SE)
- ORCID
- Ohlsson, C
- Venue
- Journal of Endocrinology
- Publisher
- Bioscientifica
- Published
- 1998-10-01
- Volume
- 159
- Issue
- 1
- Pages
- 191-195
- ISSN
- 0022-0795 (ISSN-L)
- 1479-6805
- OpenAlex
- W2163584089
- Cited by
- 185
- References
- 28
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 2 · 2023: 3 · 2022: 5 · 2021: 1 · 2020: 1 · 2019: 1 · 2018: 3 · 2017: 1 · 2016: 5 · 2014: 3 · 2013: 2 · 2012: 11
- FWCI
- 3.72
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Endocrinology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Novo Nordisk
- Vetenskapsrådet
- Novo Nordisk Fonden
- Medical Research Council
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
- Health Sciences › Medicine › Orthopedics and Sports Medicine › Bone health and osteoporosis research
- Health Sciences › Medicine › Rheumatology › Bone and Dental Protein Studies
- Keywords
- osteoprotegerin, osteoblasts, osteoblast-like cells, glucocorticoids, cortisol, mRNA expression, osteoclast differentiation inhibition, bone resorption, mesenchymal stem cells, osteosarcoma cell lines
- MeSH terms
- Anti-Inflammatory Agents, Cells, Cultured, Densitometry, Dose-Response Relationship, Drug, Estradiol, Gene Expression Regulation, Genetic Techniques, Glycoproteins, Humans, Hydrocortisone, Osteoblasts, Stimulation, Chemical, Testosterone, Time Factors, Tumor Cells, Cultured, Receptors, Tumor Necrosis Factor, Receptors, Cytoplasmic and Nuclear, Osteoprotegerin
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