Cited by 787Bone Metabolism and DiseasesBone and Dental Protein StudiesRheumatoid Arthritis Research and Therapies

Isolation of a novel cytokine from human fibroblasts that specifically inhibits osteoclastogenesis.

Tsuda, Eisuke, Goto, Masaaki, Mochizuki, Shin-ichi, Yano, Kazuki, Kobayashi, Fumie, Morinaga, Tomonori, Higashio, Kanji·1997·Biochemical and Biophysical Research Communications

Abstract

A factor which inhibits osteoclast-like cell formation was found in the conditioned medium of human embryonic lung fibroblasts, IMR-90. The factor, termed osteoclastogenesis inhibitory factor, OCIF, was purified to homogeneity. OCIF is a heparin-binding basic glycoprotein and has been isolated as a monomer with an apparent molecular weight (Mr) of 60,000 and a homodimer with a Mr of 120,000. The N-terminus of OCIF is blocked and the determination of internal amino acid sequences revealed that OCIF has no homology to known proteins. OCIF inhibited in a dose-dependent manner osteoclastogenesis elicited through three distinct signaling pathways stimulated by 1 alpha,25-dihydroxy vitamin D3, parathyroid hormone, and interleukin-11, respectively, in a dose range of 1 to 40 ng/ml (IC50 = 4 to 6 ng/ml). OCIF neither inhibits bone resorption by mature osteoclasts nor exerts any other biological activities. These data strongly suggest that OCIF is a novel cytokine which specifically inhibits osteoclastogenesis.

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Crossref · 10.1006/bbrc.1997.6603

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Authors
Tsuda, E., Goto, M., Mochizuki, S.-I., Yano, K., Kobayashi, F., Morinaga, T., & Higashio, K.
Affiliations
Megmilk Snow Brand (Japan) (JP)
Corresponding author
Tsuda, Eisuke
Venue
Biochemical and Biophysical Research Communications
Publisher
Elsevier BV
Published
1997-05
Volume
234
Issue
1
Pages
137-142
ISSN
0006-291X (ISSN-L)
OpenAlex
W2063676540
Cited by
787
References
23
Citations by year
2026: 6 · 2025: 3 · 2024: 9 · 2023: 8 · 2022: 10 · 2021: 11 · 2020: 18 · 2019: 17 · 2018: 20 · 2017: 14 · 2016: 19 · 2015: 22 · 2014: 19 · 2013: 29 · 2012: 28
FWCI
11.73
Type
journal article
Language
en
Open access
closed
Locations
  • Biochemical and Biophysical Research Communications (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
  • Health Sciences › Medicine › Rheumatology › Bone and Dental Protein Studies
  • Health Sciences › Medicine › Rheumatology › Rheumatoid Arthritis Research and Therapies
Keywords
osteoprotegerin, osteoclastogenesis inhibition, human embryonic lung fibroblasts, homodimer, 1,25-dihydroxyvitamin D3, parathyroid hormone, interleukin-11, bone resorption, conditioned medium
MeSH terms
Acid Phosphatase, Animals, Calcitriol, Cell Line, Chromatography, Affinity, Chromatography, High Pressure Liquid, Chromatography, Ion Exchange, Electrophoresis, Polyacrylamide Gel, Fibroblasts, Humans, Molecular Weight, Osteoclasts, Parathyroid Hormone, Serine Endopeptidases, Cytokines, Culture Media, Conditioned, Mice

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