Ephemeroptera, Plecoptera, and Trichoptera fauna of Churchill (Manitoba, Canada): insights into biodiversity patterns from DNA barcoding
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The insect orders Ephemeroptera, Plecoptera, and Trichoptera (EPT) are particularly important for freshwater ecological and biomonitoring studies, but difficulties in their identification to species level impede research. DNA barcoding provides a solution to this problem by linking newly collected specimens to a reference library of authoritatively identified specimens. Here, we consider the ways in which patterns of intraspecific and interspecific genetic divergences in the barcode region can provide rapid insights into the taxonomic identity, morphological features, and geographical distributions of species. Our study led to a >5× increase in the EPT fauna, including 68 caddisfly, 37 mayfly, and 7 stonefly species, recorded from Churchill. DNA barcoding also aided detection of rare taxa, allowed identification of otherwise unidentifiable life stages, revealed several potentially new species of caddisflies and mayflies, and suggested the presence of cryptic species. The new insights into this fauna and the strong congruence between morphological and molecular characters affirm the utility of DNA barcoding for rapid characterization of the diversity of EPT faunas. We also explore the phenology and habitat preferences of Churchill’s trichopterans and demonstrate that comprehensive sampling is important for documenting biodiversity through DNA barcoding.
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DNA barcoding allows for standardized and accurate species identification.
“DNA barcoding allows for standardized and accurate species identification [15–18].”
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Services of DNA barcoding in different fields
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Labiobaetis Novikova & Kluge in Borneo (Ephemeroptera, Baetidae)
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Aquatic Organisms Research with DNA Barcodes
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Stream macroinvertebrate reintroductions: A cautionary approach for restored urban streams
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Amphinemura palmeni is a valid Holarctic stonefly species (Plecoptera: Nemouridae)
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Taxonomic resolution based on DNA barcoding affects environmental signal in metacommunity structure
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DNA barcoding of mayflies (Insecta: Ephemeroptera) from South India
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The importance of taxonomic resolution for additive beta diversity as revealed through DNA barcoding
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Does monitoring of saproxylic beetles benefit from inclusion of larvae?
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New species of Indocloeon Müller-Liebenau from South-East Asia (Ephemeroptera, Baetidae)
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The Plecoptera and Trichoptera of the Arctic North Slope of Alaska
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DNA barcoding reference libraries of Italian Plecoptera: a gap analysis
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Winter-emerging chironomidae (Insecta: Diptera) in Minnesota trout streams
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Reinstatement of Leuctra biellensis Festa, 1942 (Plecoptera, Leuctridae)
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Phylogeographic comparison of five large-bodied aquatic insect species across the western USA
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Assessing arthropod biodiversity with DNA barcoding in Jinnah Garden, Lahore, Pakistan
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Self-citation
- Authors
- Zhou, X., Jacobus, L. M., DeWalt, R. E., Adamowicz, S. J., & Hebert, P. D. N.
- Affiliations
- University of Guelph (CA)
- Indiana University Bloomington (US)
- Illinois Natural History Survey (US)
- Venue
- Journal of the North American Benthological Society
- Publisher
- University of Chicago Press
- Published
- 2010-09
- Volume
- 29
- Issue
- 3
- Pages
- 814-837
- ISSN
- 0887-3593 (ISSN-L)
- 1937-237X
- OpenAlex
- W2131347012
- Cited by
- 126
- References
- 74
- Citations by year
- 2026: 3 · 2025: 2 · 2024: 11 · 2023: 2 · 2022: 7 · 2021: 12 · 2020: 10 · 2019: 7 · 2018: 12 · 2017: 10 · 2016: 13 · 2015: 8 · 2014: 8 · 2013: 7 · 2012: 5
- FWCI
- 5.80
- Type
- journal article
- Language
- en
- Open access
- green
- www.bioone.org
- Locations
- Journal of the North American Benthological Society (publishedVersion)
- BioOne Complete (BioOne) (submittedVersion)
- Funders
- Genome Canada
- University of Manitoba
- Ontario Genomics Institute
- Churchill Northern Studies Centre
- Natural Sciences and Engineering Research Council of Canada
- Topics
- Physical Sciences › Environmental Science › Ecology › Freshwater macroinvertebrate diversity and ecology
- Life Sciences › Agricultural and Biological Sciences › Ecology, Evolution, Behavior and Systematics › Invertebrate Taxonomy and Ecology
- Physical Sciences › Environmental Science › Ecology › Environmental DNA in Biodiversity Studies
- Keywords
- Ephemeroptera, Plecoptera, Trichoptera, aquatic insects, DNA barcoding, species identification, cryptic species, new species, genetic divergence, Churchill, Manitoba, insect phenology, habitat preference, biodiversity documentation
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