The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Antibody validation
Open accessAbstract
Antibodies are among the most frequently used tools in basic science research and in clinical assays, but there are no universally accepted guidelines or standardized methods for determining the validity of these reagents. Furthermore, for commercially available antibodies, it is clear that what is on the label does not necessarily correspond to what is in the tube. To validate an antibody, it must be shown to be specific, selective, and reproducible in the context for which it is to be used. In this review, we highlight the common pitfalls when working with antibodies, common practices for validating antibodies, and levels of commercial antibody validation for seven vendors. Finally, we share our algorithm for antibody validation for immunohistochemistry and quantitative immunofluorescence.
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Cites
How reliable are G-protein-coupled receptor antibodies?
Michel, M. C., Wieland, T., & Tsujimoto, G. (2009) · Naunyn-Schmiedeberg's Archives of Pharmacology · doi:10.1007/s00210-009-0395-y · Cited by 306
Ten commercial antibodies for alpha-1-adrenergic receptor subtypes are nonspecific
Jensen, B. C., Swigart, P. M., & Simpson, P. C. (2009) · Naunyn-Schmiedeberg's Archives of Pharmacology · doi:10.1007/s00210-008-0368-6 · Cited by 176
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Multiplexed ion beam imaging of human breast tumors
Angelo, M., Bendall, S. C., Finck, R., Hale, M. B., Hitzman, C., Borowsky, A. D., Levenson, R. M., Lowe, J. B., Liu, S. D., Zhao, S., et al. (2014) · Nature Medicine · doi:10.1038/nm.3488 · Cited by 1,108
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Dunn, M. R., Jimenez, R. M., & Chaput, J. C. (2017) · Nature Reviews Chemistry · doi:10.1038/s41570-017-0076 · Cited by 938
Reproducibility crisis: Blame it on the antibodies
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A proposal for validation of antibodies
Uhlen, M., Bandrowski, A., Carr, S., Edwards, A., Ellenberg, J., Lundberg, E., Rimm, D. L., Rodriguez, H., Hiltke, T., Snyder, M., et al. (2016) · Nature Methods · doi:10.1038/nmeth.3995 · Cited by 626
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Metadata
- Authors
- Bordeaux, J., Welsh, A. W., Agarwal, S., Killiam, E., Baquero, M. T., Hanna, J. A., Anagnostou, V. K., & Rimm, D. L.
- Venue
- BioTechniques
- Publisher
- Informa UK Limited
- Published
- 2010-03
- Volume
- 48
- Issue
- 3
- Pages
- 197-209
- ISSN
- 0736-6205 (ISSN-L)
- 1940-9818
- OpenAlex
- W4240422451
- Cited by
- 589
- References
- 2
- FWCI
- 16.39
- Type
- journal article
- Language
- en
- Open access
- gold
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- Bordeaux, J., Welsh, A. W., Agarwal, S., Killiam, E., Baquero, M. T., Hanna, J. A., Anagnostou, V. K., & Rimm, D. L. (2010). Antibody validation. BioTechniques. https://doi.org/10.2144/000113382
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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