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Cited by 445Nanoparticle-Based Drug DeliveryNanoparticles: synthesis and applicationsBarrier Structure and Function Studies

Iron oxide nanoparticles induce human microvascular endothelial cell permeability through reactive oxygen species production and microtubule remodeling

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Apopa, Patrick L, Qian, Yong, Shao, Rong, Guo, Nancy Lan, Schwegler-Berry, Diane, Pacurari, Maricica, Porter, Dale, Shi, Xianglin +3 more·2009·Particle and Fibre Toxicology
DOI 10.1186/1743-8977-6-1PMC PMC2632982
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Abstract

Background: Engineered iron nanoparticles are being explored for the development of biomedical applications and many other industry purposes. However, to date little is known concerning the precise mechanisms of translocation of iron nanoparticles into targeted tissues and organs from blood circulation, as well as the underlying implications of potential harmful health effects in human. Results: The confocal microscopy imaging analysis demonstrates that exposure to engineered iron nanoparticles induces an increase in cell permeability in human microvascular endothelial cells. Our studies further reveal iron nanoparticles enhance the permeability through the production of reactive oxygen species (ROS) and the stabilization of microtubules. We also showed Akt/GSK-3β signaling pathways are involved in iron nanoparticle-induced cell permeability. The inhibition of ROS demonstrate ROS play a major role in regulating Akt/GSK-3β – mediated cell permeability upon iron nanoparticle exposure. These results provide new insights into the bioreactivity of engineered iron nanoparticles which can inform potential applications in medical imaging or drug delivery. Conclusion: Our results indicate that exposure to iron nanoparticles induces an increase in endothelial cell permeability through ROS oxidative stress-modulated microtubule remodeling. The findings from this study provide new understandings on the effects of nanoparticles on vascular transport of macromolecules and drugs.

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  • In an in vitro study, iron-nanoparticles induced an increase in endothelial cell permeability through the production of ROS.

    “In an in vitro study, Apopa et al., (2009) have demonstrated that iron-nanoparticles induced an increase in endothelial cell permeability through the production of ROS.”

    From Iron, Oxidative Stress and Health (2012) · cites this paper as Apopa et al., (2009)

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Authors
Apopa, P. L., Qian, Y., Shao, R., Guo, N. L., Schwegler-Berry, D., Pacurari, M., Porter, D., Shi, X., Vallyathan, V., Castranova, V., & Flynn, D. C.
Affiliations
  • National Institutes of Health (US)
  • Baystate Medical Center (US)
  • West Virginia University (US)
  • Geisinger College of Health Sciences (US)
Corresponding author
  • Qian, Yong
  • Flynn, Daniel C
ORCID
Venue
Particle and Fibre Toxicology
Publisher
Springer Science and Business Media LLC
Published
2009-01-09
Volume
6
Issue
1
Pages
1-1
ISSN
1743-8977 (ISSN-L)
DOI
10.1186/1743-8977-6-1
PMC
PMC2632982
OpenAlex
W2158407007
Cited by
445
References
0
Citations by year
2026: 3 · 2025: 8 · 2024: 9 · 2023: 9 · 2022: 17 · 2021: 32 · 2020: 24 · 2019: 22 · 2018: 19 · 2017: 39 · 2016: 49 · 2015: 31 · 2014: 41 · 2013: 39 · 2012: 35
FWCI
15.09
Type
journal article
Language
en
Open access
Licence
cc-by
Locations
  • Particle and Fibre Toxicology (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • The Research Repository @ WVU (West Virginia University) (submittedVersion, cc-by)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
Funders
  • National Institutes of Health (RR16440)
  • National Cancer Institute (R01CA060731)
  • U.S. National Library of Medicine (R01LM009500)
  • National Center for Research Resources (P20RR016440)
Topics
  • Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
  • Physical Sciences › Materials Science › Materials Chemistry › Nanoparticles: synthesis and applications
  • Life Sciences › Neuroscience › Neurology › Barrier Structure and Function Studies
Keywords
iron oxide nanoparticles, engineered nanoparticles, endothelial permeability, reactive oxygen species, oxidative stress, microtubule stabilization, Akt/GSK-3β signaling, vascular drug delivery, nanoparticle toxicity

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