PLCL/PCL Dressings with Platelet Lysate and Growth Factors Embedded in Fibrin for Chronic Wound Regeneration
Open accessAbstract
Introduction: The formation of diabetic ulcers (DU) is a common complication for diabetic patients resulting in serious chronic wounds. There is therefore, an urgent need for complex treatment of this problem. This study examines a bioactive wound dressing of a biodegradable electrospun nanofibrous blend of poly(L-lactide-co-ε-caprolactone) and poly(ε-caprolactone) (PLCL/PCL) covered by a thin fibrin layer for sustained delivery of bioactive molecules. Methods: Electrospun PLCL/PCL nanofibers were coated with fibrin-based coating prepared by a controlled technique and enriched with human platelet lysate (hPL), fibroblast growth factor 2 (FGF), and vascular endothelial growth factor (VEGF). The coating was characterized by scanning electron microscopy and fluorescent microscopy. Protein content and its release rate and the effect on human saphenous vein endothelial cells (HSVEC) were evaluated. Results: The highest protein amount is achieved by the coating of PLCL/PCL with a fibrin mesh containing 20% v/v hPL (NF20). The fibrin coating serves as an excellent scaffold to accumulate bioactive molecules from hPL such as PDGF-BB, fibronectin (Fn), and α-2 antiplasmin. The NF20 coating shows both fast and a sustained release of the attached bioactive molecules (Fn, VEGF, FGF). The dressing significantly increases the viability of human saphenous vein endothelial cells (HSVECs) cultivated on a collagen-based wound model. The exogenous addition of FGF and VEGF during the coating procedure further increases the HSVECs viability. In addition, the presence of α-2 antiplasmin significantly stabilizes the fibrin mesh and prevents its cleavage by plasmin. Discussion: The NF20 coating supplemented with FGF and VEGF provides a promising wound dressing for the complex treatment of DU. The incorporation of various bioactive molecules from hPL and growth factors has great potential to support the healing processes by providing appropriate stimuli in the chronic wound.
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PLCL surface modification or covalent binding of bioactive molecules such as cell adhesion factors and cell growth factors can further improve compatibility with specific cells.
“PLCL surface modification or covalent binding of bioactive molecules, such as cell adhesion factors and cell growth factors, can further improve their compatibility with specific cells [25–27].”
From Application of PLCL as a biodegradable polymer in biomedical engineering (2026) · cites this paper as [26]
The Introduction and Conclusion (pages not provided) describe PLCL/PCL nanofibers modified with a fibrin coating containing bioactive molecules. The Cell Viability results report improved growth of human endothelial cells with platelet lysate and added growth factors. The paper does not establish covalent binding of those molecules to PLCL/PCL, so that alternative in the claim is unsupported. (LLM, conf=0.86)
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Application of PLCL as a biodegradable polymer in biomedical engineering
Zhao, Y., & Liu, H. (2026) · Journal of Materials Science Materials in Medicine · doi:10.1007/s10856-026-07016-3 · Cited by 7 · see citing works
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- Authors
- Táborská, J., Blanquer, A., Brynda, E., Filová, E., Stiborová, L., Jenčová, V., Havlíčková, K., Riedelová, Z., & Riedel, T.
- Affiliations
- Czech Academy of Sciences (CZ)
- Universitat Autònoma de Barcelona (ES)
- Technical University of Liberec (CZ)
- Corresponding author
- Riedel, Tomáš
- Venue
- International Journal of Nanomedicine
- Publisher
- Informa UK Limited
- Published
- 2023-02
- Volume
- Volume 18
- Pages
- 595-610
- ISSN
- 1176-9114 (ISSN-L)
- 1178-2013
- PMC
- PMC9904224
- OpenAlex
- W4319791703
- Cited by
- 21
- References
- 52
- Citations by year
- 2026: 4 · 2025: 8 · 2024: 6 · 2023: 3
- FWCI
- 2.30
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- International Journal of Nanomedicine (publishedVersion, cc-by-nc)
- PubMed Central (publishedVersion, cc-by-nc)
- PubMed (publishedVersion)
- Dipòsit Digital de Documents de la UAB (Universitat Autònoma de Barcelona) (submittedVersion, cc-by-nc)
- Dove Medical Press (Taylor and Francis Group) (submittedVersion, cc-by-nc)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- European Commission (LX22NPO5104)
- Ministerstvo Zdravotnictví Ceské Republiky (LX22NPO5104)
- Agentura Pro Zdravotnický Výzkum České Republiky (LX22NPO5104, NV18-01-00332)
- NextGenerationEU
- Topics
- Health Sciences › Medicine › Rehabilitation › Wound Healing and Treatments
- Physical Sciences › Materials Science › Biomaterials › Electrospun Nanofibers in Biomedical Applications
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Keywords
- fibrin coating, platelet lysate, growth factor delivery, diabetic ulcers, chronic wound healing, electrospun nanofibers, fibroblast growth factor 2, vascular endothelial growth factor, alpha2-antiplasmin, endothelial cell viability, sustained protein release, wound dressings
- MeSH terms
- alpha-2-Antiplasmin, Bandages, Humans, Polyesters, Wound Healing, Vascular Endothelial Growth Factor A, Endothelial Cells, Nanofibers
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