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Cited by 106

The DOI matches its Crossref record; 15 of the 15 references it cites were found in a registry or an index.

State-of-the-Art Lentiviral Vectors for Research Use: Risk Assessment and Biosafety Recommendations

Pauwels, Katia, Gijsbers, Rik, Toelen, Jaan, Schambach, Axel, Willard-Gallo, Karen, Verheust, Celine, Debyser, Zeger, Herman, Philippe·2009·Current Gene Therapy
DOI 10.2174/156652309790031120
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Abstract

Lentiviral vectors (LV) are competent gene transfer vehicles, as used for both research and gene therapy applications, because of their stable integration in non-dividing and dividing cells and long-term transgene expression. Along with our understanding that LV offer solutions for gene therapy, biosafety concerns have uncovered risks due to insertional mutagenesis, the generation of replication competent lentiviruses (RCL) and vector mobilization. Researchers therefore continue to devote significant efforts in designing LV with improved efficacy and biosafety features. The choice of a particular LV system for experimental studies is often driven by functional considerations, including increased productivity and/or transduction efficiency. The design of safer vectors has also directly benefited researchers allowing them to conduct experimental studies with lower risk. Currently, vectors combine improved safety features (that decrease the risk of recombination and vector mobilization) with increased transduction efficiency. Hence, risks associated with the inadvertent transduction of cells of the investigator gain greater importance in assessing the overall risk of these vectors and become an important biosafety concern. This review outlines the different strategies used to improve LV biosafety by comparing state-of-the-art and emerging LV production systems and highlighting biosafety issues that can arise during their contained use. The few existing national and international…

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Metadata

Authors
Pauwels, K., Gijsbers, R., Toelen, J., Schambach, A., Willard-Gallo, K., Verheust, C., Debyser, Z., & Herman, P.
Venue
Current Gene Therapy
Publisher
Bentham Science Publishers Ltd.
Published
2009-12-01
Volume
9
Issue
6
Pages
459-474
ISSN
1566-5232
DOI
10.2174/156652309790031120
Cited by
106
References
15
Language
en

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