Isolation, antioxidant and antimicrobial activities of ecdysteroids from Serratula cichoracea
Abstract
Background: Several Serratula species are used in folk medicine and showed interesting biological activities. The objective of this work was to continue the investigation of the chemical composition of the ethyl acetate extract of the flowers of Serratula cichoracea which in our previous study showed a significant antioxidant activity in 1,1-diphenyl-2-picrylhydrazyl radical (DPPH•) essay compared to quercetin used as control molecule and to evaluate the isolated compounds for their antioxidant and antimicrobial activities. Methods: Ethyl acetate extract (15 g) was fractionated by column chromatography on silica gel. After purification on preparative plates, the isolated compounds were identified by spectral analyses mainly high resolution electrospray ionisation mass spectrometry (HR-ESIMS) and one- and two-dimensionnal nuclear magnetic resonance spectroscopy experiments, and were evaluated in vitro for antioxidant activity using 1,1-diphenyl-2-picrylhydrazyl radical and for antimicrobial properties. Results: A new phytoecdysteroid named 22-epi-ajugasterone C (1) together with ajugasterone C (2), were isolated. Compounds 1 and 2 had weak scavenging effect compared with myricetin used as positive control, whereas the two compounds showed antimicrobial properties against multiresistant strains like Staphylococcus aureus, Escherichia coli, Serratia sp., Klebsiella pneumoniae and Candida albicans. Conclusion: The new isolated phytoecdysteroid and its epimer ajugasterone C, responded to their effectiveness against different various antibiotic-resistant bacteria. Keywords: Serratula cichoracea, Asteraceae, phytoecdysteroids, antimicrobial, antioxidant activities, chromatography.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
22-epi-Ajugasterone C showed weak DPPH radical-scavenging and antimicrobial activity against multi-resistant Gram-positive bacteria, Gram-negative bacteria, and yeasts.
“Weak DPPH radical-scavenging and antimicrobial activity against multi resistant strains Gram positive bacteria (Staphylococcus aureus 118), Gram negative bacteria such as (Escherichia coli 113, Klebsiella pneumoniae 112, Proteus mirabilis 23, Serratia sp. 2028, Pseudomonas aurogenosa43) and yeasts (Candida albicans).”
From The Phytochemical, Biological, and Medicinal Attributes of Phytoecdysteroids: An Updated Review (2021) · cites this paper as [68]
22-epi-Ajugasterone C showed in vitro antioxidant activity by DPPH radical scavenging assay.
“PE 22-epi-ajugasterone C (62) also showed in vitro antioxidant activity by DPPH radical scanging assay68.”
From The Phytochemical, Biological, and Medicinal Attributes of Phytoecdysteroids: An Updated Review (2021) · cites this paper as [68]
PEs display antimicrobial properties against multi-resistant strains such as Escherichia coli, Staphylococcus aureus, Candida albicans, and Klebsiella pneumoniae.
“PEs display antimicrobial properties against multi-resistant strains such as Escherichia coli, Staphylococcus aureus, Candida albicans, and Klebsiella pneumoniae [60].”
From Phytoecdysteroids: Distribution, Structural Diversity, Biosynthesis, Activity, and Crosstalk with Phytohormones (2022) · cites this paper as [60]
Cited by
Sorted by citations
The Phytochemical, Biological, and Medicinal Attributes of Phytoecdysteroids: An Updated Review
Das, N., Mishra, S. K., Bishayee, A., Ali, E. S., & Bishayee, A. (2021) · Acta Pharmaceutica Sinica B · doi:10.1016/j.apsb.2020.10.012 · Cited by 115 · see citing works
Arif, Y., Singh, P., Bajguz, A., & Hayat, S. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms23158664 · Cited by 50 · see citing works
“Serratula cichoracea 22-epi-ajugasterone C [60]”
BackgroundEcdysteroids: Isolation, Chemical Transformations, and Biological Activity
Savchenko, R. G., Veskina, N. A., Odinokov, V. N., Benkovskaya, G. V., & Parfenova, L. V. (2022) · Phytochemistry Reviews · doi:10.1007/s11101-021-09792-y · Cited by 39 · see citing works
“The compounds exhibited antimicrobial properties against multiple resistant strains such as Staphylococcus aureus, Escherichia coli, Serratia sp., Klebsiella pneumoniae, and Candida albicans (Aliouche et al., 2018).”
BackgroundBioactive Compounds and Biological Activity of Croton Species (Euphorbiaceae): An Overview
Bezerra, F. W. F., Bezerra, P. D. N., de Oliveira, M. S., et al. (2018) · Current Bioactive Compounds · doi:10.2174/1573407215666181122103511 · Cited by 13 · see citing works
Park, I., Park, K., Lee, H.-S., Hong, S.-M., Sriramulu, D. K., Hwang, H., Song, S., Baek, J. G., Kim, D. H., Kim, S. Y., et al. (2023) · ACS Omega · doi:10.1021/acsomega.3c02421 · Cited by 11 · see citing works
Ecdysteroids as defensive chemicals
(2022) · Advances in Insect Physiology · doi:10.1016/bs.aiip.2022.09.001 · Cited by 11 · see citing works
Olennikov, D. N. (2022) · Separations · doi:10.3390/separations9120448 · Cited by 7 · see citing works
Aliouche, L., Mosset, P., León, F., et al. (2019) · Current Bioactive Compounds · doi:10.2174/1573407215666190213125259 · Cited by 4 · see citing works
Self-citationSzerencsés, B., Vörös, M., Bagi, K., Háznagy, M. B., Hunyadi, A., Vágvölgyi, C., Pfeiffer, I., & Vágvölgyi, M. (2022) · Microbiology Research · doi:10.3390/microbiolres13040071 · Cited by 4 · see citing works
RANI, S., Gahlot, K., & Kumar, A. (2020) · Asian Journal of Pharmaceutical and Clinical Research · doi:10.22159/ajpcr.2020.v13i4.36836 · Cited by 3 · see citing works
Ningsih, D. R., Zusfahair, Z., Hermawan, D., et al. (2020) · Current Bioactive Compounds · doi:10.2174/1573407216666200214100855 · Cited by 3 · see citing works
Two undescribed phytoecdysteroids from Dianthus superbus L.
Liu, Y.-H., Zhang, Q., Zhang, D.-Z., Fu, X.-Y., Liu, W.-Y., & Tian, J. (2025) · Phytochemistry Letters · doi:10.1016/j.phytol.2025.103015 · Cited by 1 · see citing works
Boreiko, S., Machado, A. T. P., Stiirmer, J. C., et al. (2018) · Current Bioactive Compounds · doi:10.2174/1573407214666180803140947 · Cited by 1 · see citing works
Amrani, A., Boubekri, N., Benaissa, O., et al. (2019) · Current Bioactive Compounds · doi:10.2174/1573407215666190308152505 · Cited by 1 · see citing works
Self-citationHodyna, D. M., Kovalishyn, V., Semenyuta, I. V., et al. (2020) · Current Bioactive Compounds · doi:10.2174/1573407216999200422115655
Antimikrobiális ekdiszteroidok félszintézise és parazitaellenes aktivitásuk vizsgálata
Háznagy, M. B. (2023) · Az SZTE Gyógyszerésztudományi Kar ÚNKP díjazottjainak tudományos előadóülése · doi:10.14232/gytk.unkp.2023.8
Characterization and bioactivity of β- ecdysterone from cell cultures of Trianthema decandra L.
Pancholi, B. (2023) · Indian Journal of Natural Products and Resources · doi:10.56042/ijnpr.v14i4.6167
“Ecdysteroids were reported with potent antimicrobial activity in several antibiotic resistant microorganism such as Staphylococcus aureus , Escherichia coli , Serratia sp., Klebsiella pneumoniae , and Candida albicans 28-31 .”
BackgroundLing, S.-S., Zhang, Y., Li, Y.-X., Wei Min, N. I., Wang, M.-R., Fang, X., Huang, X., & Liu, H. (2026) · Fitoterapia · doi:10.1016/j.fitote.2026.107185
Tuleuov, B. I., Zeinuldina, A., Bazarkhankyzy, A., Kozhanova, A. M., Temirgaziev, B. S., Dyussekeyeva, S., Abulyaissova, L. K., Askarova, N., Теmirbekova, A., Борамбаевна, Т. Ж., et al. (2026) · Pharmaceuticals · doi:10.3390/ph19060885 · Cited by 1 · see citing works
“Agar diffusion is strongly influenced by compound solubility, molecular size, and the ability to diffuse through a solid medium, whereas the MIC method more accurately reflects the ability of a substance to completely inhibit microbial growth in a liquid phase [36,37].”
Antioxidant and Anti-Inflammatory Activities of Phytoecdysteroids from Vitex madiensis (Oliv.)
Boungou-Tsona, G., Decombat, C., Bikindou, K., et al. (2026) · Molecules · doi:10.3390/molecules31122110
Seydmohammadi, L., Borna, F., Negaresh, K., et al. (2026) · Natural Product Research · doi:10.1080/14786419.2026.2690080
- Authors
- Aliouche, L., Larguet, H., Amrani, A., Leon, F., Brouard, I., Benayache, S., Zama, D., Meraihi, Z., & Benayache, F.
- Affiliations
- University Frères Mentouri Constantine 1 (DZ)
- Instituto de ProductosNaturales y Agrobiologia-C.S.I.C., Av. Astrofisico Francisco Sanchez 3, 38206 La Laguna, Tenerife, Spain
- Instituto de Productos Naturales y Agrobiología (ES)
- Laboratoire de Genie Microbiologique et Applications, Faculte des Sciences de la Nature et de la Vie, Departement de Biochimie et Biologie Cellulaire & Moleculaire, Universite Freres Mentouri Constantine 1, Route d`Ain El Bey 25017, Algeria
- Corresponding author
- Benayache, Fadila
- Venue
- Current Bioactive Compounds
- Publisher
- Bentham Science Publishers Ltd.
- Published
- 2018-04-30
- Volume
- 14
- Issue
- 1
- Pages
- 60-66
- ISSN
- 1573-4072 (ISSN-L)
- OpenAlex
- W2774433130
- Cited by
- 21
- References
- 0
- Citations by year
- 2026: 4 · 2025: 1 · 2023: 3 · 2022: 5 · 2020: 4 · 2019: 2 · 2018: 2
- FWCI
- 0.89
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Current Bioactive Compounds (publishedVersion)
- DIGITAL.CSIC (Spanish National Research Council (CSIC)) (submittedVersion)
- Funders
- Direction Générale de la Recherche Scientifique et du Développement Technologique
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Phytochemical Studies and Bioactivities
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Phytochemistry and Biological Activities
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bioactive Natural Diterpenoids Research
- Keywords
- phytoecdysteroids, ecdysteroids, antioxidant activity, antimicrobial activity, antibiotic-resistant bacteria, Staphylococcus aureus, Escherichia coli, Serratia, Klebsiella pneumoniae, Candida albicans, DPPH radical scavenging, ethyl acetate extract, flower constituents, natural product isolation
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
