Ultrasound-microwave assisted extraction of flavonoid compounds from Eucommia ulmoides leaves and an evaluation of their antioxidant and antibacterial activities
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Ultrasound/microwave assisted extraction (UMAE) of flavonoid compounds from Eucommia ulmoides leaves was studied and the extraction conditions were optimized by the Plackett-Burman design (PBD) method combined with the Box-Behnken design (BBD). The antioxidant and antibacterial activities of the flavonoid extract were investigated. The results show that the optimal conditions were an ethanol concentration of 41%, microwave power of 178 W and an ultrasound extraction time of 26 min. Under these conditions, the yield of the flavonoid compounds was 2.454%?0.230%, which was higher than that after direct solvent extraction, ultrasound extraction and microwave extraction. The results of in vitro antioxidant assays showed that the flavonoid extract had scavenging capacity for DPPH, ABTS and hydroxyl radicals, with corresponding IC50 values of 30.76 mg/L, 21.09 mg/L, 248.4 mg/L, respectively. In addition, this extract exhibited strong antibacterial activity on Escherichia coli, Staphylococcus aureus and Salmonella typhimurium.
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Wang et al. [132] found that extraction yield of UMAE was 2.454 % higher than that of ultrasonic and microwave extraction.
“The findings indicated that the extraction yield of UMAE was 2.454 % higher than that of ultrasonic and microwave extraction.”
From A comprehensive review of ultrasonic assisted extraction (UAE) for bioactive components: Principles, advantages, equipment, and combined technologies (2023) · cites this paper as [132]
The comparison-of-extraction-methods section reports 2.454% as the yield obtained with UMAE, not the percentage by which it exceeded the other methods. It says UMAE's yield was 20.24% higher than ultrasound-assisted extraction and 41.77% higher than microwave-assisted extraction. (LLM, conf=0.90)
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Cites
Thaipong, K., Boonprakob, U., Crosby, K. M., Cisneros‐Zevallos, L., & Byrne, D. (2006) · Journal of Food Composition and Analysis · doi:10.1016/j.jfca.2006.01.003 · Cited by 3,649 · see citing works
Antioxidant and radical scavenging properties of curcumin
Ak, T. P., & Gülçın, İ. (2008) · Chemico-Biological Interactions · doi:10.1016/j.cbi.2008.05.003 · Cited by 1,942 · see citing works
Gill, A. O., & Holley, R. A. (2006) · International Journal of Food Microbiology · doi:10.1016/j.ijfoodmicro.2005.10.009 · Cited by 541 · see citing works
Lianfu, Z., & Zelong, L. (2008) · Ultrasonics Sonochemistry · doi:10.1016/j.ultsonch.2007.12.001 · Cited by 405 · see citing works
Trouillas, P., Marsal, P., Siri, D., Lazzaroni, R., & Duroux, J. (2005) · Food Chemistry · doi:10.1016/j.foodchem.2005.05.042 · Cited by 361 · see citing works
Filly, A., Fernàndez, X., Minuti, M., Visinoni, F., Cravotto, G., & Chemat, F. (2013) · Food Chemistry · doi:10.1016/j.foodchem.2013.10.139 · Cited by 327 · see citing works
Proteomic Identification of Carbonylated Proteins and Their Oxidation Sites
Madian, A. G., & Regnier, F. E. (2010) · Journal of Proteome Research · doi:10.1021/pr1002609 · Cited by 271 · see citing works
Assisted extraction of rosemary antioxidants with green solvents
Rodríguez‐Rojo, S., Visentin, A., Maestri, D., & Cocero, M. J. (2011) · Journal of Food Engineering · doi:10.1016/j.jfoodeng.2011.09.029 · Cited by 263 · see citing works
Rahmanian, N., Jafari, S. M., & Wani, T. A. (2015) · Trends in Food Science & Technology · doi:10.1016/j.tifs.2014.12.009 · Cited by 262 · see citing works
Constituents and pharmacological effects of Eucommia and Siberian ginseng.
Deyama, T., Nishibe, S., & Nakazawa, Y. (2001) · PubMed · Cited by 261 · see citing works
Antibacterial properties of essential oils from Thai medicinal plants
Wannissorn, B., Jarikasem, S., Siriwangchai, T., & Thubthimthed, S. (2005) · Fitoterapia · doi:10.1016/j.fitote.2004.12.009 · Cited by 258 · see citing works
Response surface optimization of ultrasound assisted extraction of pectin from pomegranate peel
Moorthy, I. G., Maran, J. P., Surya, S. M. @., Naganyashree, S., & Shivamathi, C. S. (2015) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2014.10.037 · Cited by 256 · see citing works
Fazeli, M. R., Amin, G., Ahmadian-Attari, M. M., et al. (2006) · Food Control · doi:10.1016/j.foodcont.2006.03.002 · Cited by 250 · see citing works
Sequential ultrasound-microwave assisted acid extraction (UMAE) of pectin from pomelo peels.
Liew, S. Q., Ngoh, G., Yusoff, R., & Teoh, W. H. (2016) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2016.08.065 · Cited by 213 · see citing works
Microwave assisted extraction of pectin from waste Citrullus lanatus fruit rinds
Prakash Maran, J., Sivakumar, V., Thirugnanasambandham, K., & Sridhar, R. (2014) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2013.09.062 · Cited by 211 · see citing works
Zhang, G., He, L., & Hu, M. (2010) · Innovative Food Science & Emerging Technologies · doi:10.1016/j.ifset.2010.12.003 · Cited by 206 · see citing works
Chen, Y., Gu, X., Huang, S., Li, J., Wang, X., & Tang, J. (2010) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2010.02.003 · Cited by 186 · see citing works
Self-citationMehmood, A., Ishaq, M., Zhao, L., Yaqoob, S., Safdar, B., Nadeem, M., Munir, M., & Wang, C. (2019) · Ultrasonics Sonochemistry · doi:10.1016/j.ultsonch.2018.10.013 · Cited by 184 · cited 1× · see citing works
“To compensate for these deficiencies, novel technologies have been devised for the extraction of bioactive compounds, including ultrasound and microwaves [9].”
BackgroundPan, G., Yu, G., Zhu, C., & Qiao, J. (2011) · Ultrasonics Sonochemistry · doi:10.1016/j.ultsonch.2011.11.006 · Cited by 179 · see citing works
Gharibzahedi, S. M. T., Smith, B. M., & Guo, Y. (2019) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2019.114992 · Cited by 175 · see citing works
Dai, X., Huang, Q., Zhou, B., et al. (2013) · Food Chemistry · doi:10.1016/j.foodchem.2013.02.006 · Cited by 161 · see citing works
MICROWAVE EXTRACTION OF ACTIVE PRINCIPLES FROM MEDICINAL PLANTS
Alupului, A., & Lavric, V. (2012) · Cited by 145 · see citing works
Pinela, J., Prieto, M. A., Carvalho, A. M., Barreiro, M. F., Oliveira, M. B. P. P., Barros, L., & Ferreira, I. C. F. R. (2016) · Separation and Purification Technology · doi:10.1016/j.seppur.2016.03.030 · Cited by 143 · see citing works
Tsimogiannis, D., & Oreopoulou, V. (2005) · Innovative Food Science & Emerging Technologies · doi:10.1016/j.ifset.2005.09.001 · Cited by 128 · see citing works
Cheung, Y. C., Siu, K. C., Liu, Y., et al. (2012) · Process Biochemistry · doi:10.1016/j.procbio.2012.02.004 · Cited by 112 · see citing works
Jovanović-Malinovska, R., Kuzmanova, S., & Winkelhausen, E. (2014) · Ultrasonics Sonochemistry · doi:10.1016/j.ultsonch.2014.07.016 · Cited by 107 · see citing works
Wizi, J., Wang, L., Hou, X., Tao, Y., Ma, B., & Yang, Y. (2018) · Industrial Crops and Products · doi:10.1016/j.indcrop.2018.04.068 · Cited by 101 · see citing works
Wang, Z., Xie, J., Kan, L., Wang, J., Shen, M., Li, W., Nie, S., & Xie, M. (2015) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2015.06.031 · Cited by 99 · see citing works
Self-citationHou, M., Hu, W., Xiu, Z., et al. (2019) · Journal of Chromatography B · doi:10.1016/j.jchromb.2019.121960 · Cited by 76 · see citing works
Liu, Z., Qiao, L., Yang, F., Gu, H., & Yang, L. (2016) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2016.10.028 · Cited by 67 · see citing works
Antioxidant and xanthine oxidase inhibitory activities of total polyphenols from onion
Ouyang, H., Hou, K., Peng, W., et al. (2017) · Saudi Journal of Biological Sciences · doi:10.1016/j.sjbs.2017.08.005 · Cited by 67 · see citing works
Guo, N., Zang, Y.-P., Cui, Q., Gai, Q., Jiao, J., Wang, W., Zu, Y., & Fu, Y. (2016) · Food Control · doi:10.1016/j.foodcont.2016.12.013 · Cited by 65 · see citing works
Gharibzahedi, S. M. T. (2017) · Journal of Molecular Liquids · doi:10.1016/j.molliq.2017.03.094 · Cited by 61 · see citing works
Hamed, Y. S., Abdin, M., Akhtar, H. M. S., Chen, D., Wan, P., Chen, G., & Zeng, X. (2019) · South African Journal of Botany · doi:10.1016/j.sajb.2019.05.006 · Cited by 49 · see citing works
Greenway, F. L., Liu, Z., Yu, Y., et al. (2011) · PubMed · Cited by 43 · see citing works
Xu, J., Li, M., & Sun, R.-C. (2014) · Process Biochemistry · doi:10.1016/j.procbio.2014.12.021 · Cited by 36 · see citing works
Guo, W., Meng, Z.-H., Ren, N., et al. (2011) · International Journal of Hydrogen Energy · doi:10.1016/j.ijhydene.2010.11.004 · Cited by 31 · see citing works
Zhang, C., Wang, C., Tao, R., & Fang, C. (2015) · Process Biochemistry · doi:10.1016/j.procbio.2015.12.008 · Cited by 28 · see citing works
Li, Y., Zeng, R.-J., Lü, Q., et al. (2013) · Journal of Separation Science · doi:10.1002/jssc.201300975 · Cited by 24 · see citing works
Wang, J., Bai, F., Wang, J., & Guo, J. (2015) · Advance Journal of Food Science and Technology · doi:10.19026/ajfst.7.1283 · Cited by 7 · see citing works
Study on Antimicrobial Activities of Extracts from Eucommia ulmoides Oliv. Leaves
Su, Y. (2008) · Cited by 5 · see citing works
Study on Antioxidative Activity and Ingredient of Extract from Toona Sinensis Fruit
Qing-xiong, Y. (2011) · Seed · Cited by 2 · see citing works
Liu, T. (2013) · Science and Technology of Food Industry · Cited by 2 · see citing works
Determination of total flavonoids in Brazil green propolis capsule.
YueBin, L., JinBiao, Y., SiYan, W., et al. (2015) · Shipin anquan zhiliang jiance xuebao · Cited by 1 · see citing works
Study on the ultrasonic assisted extraction of total flavonoids from Eucommia ulmoides leaves
Guipin, L. (2013) · Shipin yu fajiao gongye · Cited by 1 · see citing works
Microwave-assisted extraction process of total flavonoids from Eucommia ulmoides leaves
Guipin, L. (2015) · China Brewing · Cited by 1 · see citing works
He, Q.-H., Gong, Z., Mei, Y., et al. (2019) · Cited by 1 · see citing works
Peltonen (2000) · doi:10.1002/1097-0010(200010)80:13
Flagged first, then by citations
Research works printed but not linked: 6
- Zhong SJ, Yang X, Li J, Li Y, Li XS. Study on the Total Flavo- noids Content and Antioxidant Activity in Different Parts of Eucommiae ulmoides. China Pharmacy. 2017;28(13):1787-90.
- Parvathy KS, Negi PS, Srinivas P. Antioxidant, antimutagenic and antibacterial activities of curcumin-β-diglucoside. Food Chem. 2008;115(1):265-71.
- Sandrine PI, Christian G, Giancarlo C, Farid C. A com- parison of essential oil obtained from lavandin via different extraction processes: ultrasound, microwave, turbohydro- distillation, steam and hydrodistillation. J Chromatogra A. 2013;1305(24):41-7.
- Gharibzahedi SMT., Razavi S H, Mousavi M. Optimisation and kinetic studies on the production of intracellular can- thaxanthin in fed-batch cultures of Dietzia natronolimnaea HS-1. Qual Assur Saf Crop. 2015;7(5):757-67.
- Frankel EN, Meyer AS. The problems of using one-dimen- sional methods to evaluate multifunctional food and bio- logical antioxidants. J Sci Food Agr. 2000; 80(13):1925-41.
- Lu X, Zheng Z, Li H, Cao R, Zheng YF, Yue H, Xiao JB, Miao S, Zheng BD. Optimization of ultrasonic-microwave assisted extraction of oligosaccharides from lotus (Nelumbo nucifera, Gaertn.). Ind Crop Prod. 2017;107:546-57.
Cited by
Sorted by citations
Shen, L., Pang, S., Zhong, M., Sun, Y., Qayum, A., Liu, Y., Rashid, A., Xu, B., Liang, Q., Ma, H., et al. (2023) · Ultrasonics Sonochemistry · doi:10.1016/j.ultsonch.2023.106646 · Cited by 892 · see citing works
“Also, Wang et al. [132] used UMAE to extract flavonoid compounds from Eucommia ulmoides leaves.”
Background
- Authors
- Wang, X., Peng, M.-J., Wang, Z.-H., Yang, Q.-L., & Peng, S.
- Affiliations
- Jishou University (CN)
- Guangdong Provincial Public Laboratory of Analysis and Testing Technology, Guangdong Institute of Analysis, Guangzhou, China
- Corresponding author
- Peng, Sheng
- Venue
- Archives of Biological Sciences
- Publisher
- National Library of Serbia
- Published
- 2020
- Volume
- 72
- Issue
- 2
- Pages
- 211-221
- ISSN
- 0354-4664 (ISSN-L)
- 1821-4339
- OpenAlex
- W3018049927
- Cited by
- 34
- References
- 48
- Citations by year
- 2026: 5 · 2025: 5 · 2024: 6 · 2023: 3 · 2022: 9 · 2021: 5 · 2020: 1
- FWCI
- 2.44
- Type
- journal article
- Language
- en
- Open access
- diamond
- doi.org
- Licence
- Locations
- Archives of Biological Sciences (publishedVersion, cc-by-nc-nd)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- National Natural Science Foundation of China (2019GDASYL-0502003, 31660181)
- Guangdong Academy of Sciences
- Topics
- Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
- Life Sciences › Agricultural and Biological Sciences › Food Science › Agricultural and Biological Research
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Phytochemistry and Biological Activities
- Keywords
- Eucommia ulmoides, flavonoids, ultrasound-assisted extraction, microwave-assisted extraction, extraction optimization, antioxidant activity, antibacterial activity, DPPH radical scavenging, ABTS radical scavenging, hydroxyl radical scavenging, Escherichia coli, Staphylococcus aureus, Salmonella typhimurium
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