Cited by 184Phytochemicals and Antioxidant ActivitiesAgricultural and Biological ResearchUltrasound and Cavitation Phenomena

Impact of ultrasound and conventional extraction techniques on bioactive compounds and biological activities of blue butterfly pea flower (Clitoria ternatea L.)

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Mehmood, Arshad, Ishaq, Muhammad, Zhao, Lei, Yaqoob, Sanabil, Safdar, Bushra, Nadeem, Muhammad, Munir, Masooma, Wang, Chengtao·2019·Ultrasonics Sonochemistry

Abstract

The present study was conducted to evaluate the influence of ultrasound on bioactive compounds and biological activities of blue butterfly pea flower (Clitoria ternatea L.). For this purpose, optimized conditions (temperature 50 °C, time 150 min, solid to liquid ratio 1 g:15 ml, 70% amplitude and 240 W, 20 kHz frequency, 3 s on and 3 s off) of ultrasound (US) and conventional extraction (AGE: Agitation, water bath for 150 min, 50 °C at 150 rmp) were used. The results showed significant (p < 0.05) effect of US and AGE on total phenolics (TPCs), flavonoids (TFCs) and antioxidant activities (DPPH, ABTS, FRAP, reducing activity, Cu2+ and H2O2) of butterfly pea flower extract (BPFE). The results showed an increased trend in yield, TPCs, TFCs and antioxidant activities of US treated BPFE with comparison to AGE. However, insignificant (p > 0.05) effect of US and AGE over TFlaCs and PACs were observed. Moreover, the results of Fourier-transform infrared spectroscopy (FTIR) showed little changes in spectrum and US does not affect the functional group of bioactive compounds structure. Additionally, extracts (500–2000 µg/ml) protect pBR322 plasmid DNA damage induced by (1 mM H2O2 and 1 mM FeSO4), plasma oxidation (induced by 250 µM CuCl2) and inhibit erythrocyte hemolysis (induced by 200 mM AAPH, 34.6 to 66.73%). Sonication can be applied successfully for the extraction of bioactive compounds from plant materials with high biological activities.

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Crossref · 10.1016/j.ultsonch.2018.10.013

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Authors
Mehmood, A., Ishaq, M., Zhao, L., Yaqoob, S., Safdar, B., Nadeem, M., Munir, M., & Wang, C.
Affiliations
  • Beijing Technology and Business University (CN)
  • Jilin Agricultural University (CN)
  • University of Sargodha (PK)
Corresponding author
  • Zhao, Lei
  • Munir, Masooma
  • Wang, Chengtao
Venue
Ultrasonics Sonochemistry
Publisher
Elsevier BV
Published
2019-03
Volume
51
Pages
12-19
ISSN
1350-4177 (ISSN-L)
OpenAlex
W2896976712
Cited by
184
References
69
Citations by year
2026: 17 · 2025: 18 · 2024: 37 · 2023: 37 · 2022: 24 · 2021: 20 · 2020: 19 · 2019: 11 · 2018: 1
FWCI
8.77
Type
journal article
Language
en
Open access
Locations
  • Ultrasonics Sonochemistry (publishedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
  • OSTI OAI (U.S. Department of Energy Office of Scientific and Technical Information) (submittedVersion)
Funders
  • National key research and development program (2018YFD0400403, 2016YFD0400802)
  • National Natural Science Foundation of China (31571801)
Topics
  • Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Agricultural and Biological Research
  • Physical Sciences › Materials Science › Materials Chemistry › Ultrasound and Cavitation Phenomena
Keywords
ultrasound-assisted extraction, Clitoria ternatea, butterfly pea flower, bioactive compounds, total phenolic content, flavonoids, antioxidant activity, free radical scavenging, DNA protection, extraction optimization, plant extracts
MeSH terms
Ultrasonic Waves, Animals, Antioxidants, Erythrocytes, Chemical Fractionation, Hemolysis, Oxidation-Reduction, Phenols, Plant Extracts, Temperature, Xanthine Oxidase, Clitoria, Proanthocyanidins

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