Modulation of Adipose Tissue Expression of Murine Matrix Metalloproteinases and Their Tissue Inhibitors With Obesity
Open accessAbstract
The potential role of the matrix metalloproteinase (MMP) system in the pathophysiology of the adipose tissue was investigated in a mouse model of nutritionally induced obesity. mRNA levels of 16 MMPs and 4 tissue inhibitors of MMPs (TIMPs) were measured by semiquantitative RT-PCR in adipose tissue isolated from mice maintained for 15 weeks on a standard or high-fat diet. In mice on standard diet, with the exception of MMP-8, all MMP and TIMP transcripts were detected in both gonadal and subcutaneous depots. In obese mice, the expression of MMP-3, -11, -12, -13, and -14 and TIMP-1 mRNAs was upregulated, whereas that of MMP-7, -9, -16, and -24 and TIMP-4 was downregulated. Most MMP and TIMP mRNAs were expressed at higher levels in stromal-vascular cells than in mature adipocytes. Analysis of adipose tissue by in situ fluorescent zymography revealed MMP-dependent proteolytic activities, demonstrating the presence of active MMPs in the intact tissue. In vitro conversion of adipogenic 3T3-F442A cells into mature adipocytes was associated with substantial modulations of MMP and TIMP expression. Moreover, this in vitro adipogenesis was reduced in the presence of a synthetic MMP inhibitor. Thus, the adipose tissue expresses a large array of MMPs and TIMPs, which modulate adipocyte differentiation.
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ECM components in AT are regulated by matrix metalloproteinases, a family of neutral endopeptidases that cleave ECM components, thereby enabling remodeling of ECM.
“The ECM components in AT are known to be regulated by matrix metalloproteinases, a family of neutral endopeptidases that cleave ECM components, thereby enabling remodeling of ECM (106)”
From Adipose tissue remodeling and obesity (2011) · cites this paper as [106]
In DIO mice, expression levels of MMP3, -11, -12, -13, and -14 are all upregulated, whereas levels of MMP7, -9, -16, and -24 are downregulated.
“Maquoi and colleagues further demonstrated that in DIO mice, expression levels of MMP3, -11, -12, -13, and -14 are all upregulated, whereas levels of MMP7, -9, -16, and -24 are downregulated (106)”
From Adipose tissue remodeling and obesity (2011) · cites this paper as [106]
TIMP1 is upregulated with obesity, and TIMP4 is downregulated in states of obesity.
“More specifically, TIMP1 is upregulated with obesity, and conversely, TIMP4 is downregulated in states of obesity (106)”
From Adipose tissue remodeling and obesity (2011) · cites this paper as [106]
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- Authors
- Maquoi, E., Munaut, C., Colige, A., Collen, D., & Lijnen, H. R.
- Affiliations
- KU Leuven (BE)
- University of Liège (BE)
- Center for Molecular and Vascular Biology, University of Leuven, Belgium
- Venue
- Diabetes
- Publisher
- American Diabetes Association
- Published
- 2002-04-01
- Volume
- 51
- Issue
- 4
- Pages
- 1093-1101
- ISSN
- 0012-1797 (ISSN-L)
- 1939-327X
- OpenAlex
- W2136858036
- Cited by
- 271
- References
- 37
- Citations by year
- 2026: 1 · 2025: 7 · 2024: 8 · 2023: 9 · 2022: 12 · 2021: 13 · 2020: 12 · 2019: 4 · 2018: 10 · 2017: 11 · 2016: 15 · 2015: 17 · 2014: 16 · 2013: 15 · 2012: 13
- FWCI
- 3.31
- Type
- journal article
- Language
- en
- Open access
- green
- orbi.uliege.be
- Licence
- other-oa
- Locations
- Diabetes (publishedVersion)
- PubMed (publishedVersion)
- ORBi (University of Liège) (submittedVersion, other-oa)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Protease and Inhibitor Mechanisms
- Health Sciences › Medicine › Epidemiology › Adipokines, Inflammation, and Metabolic Diseases
- Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
- Keywords
- matrix metalloproteinases, tissue inhibitors of metalloproteinases, adipose tissue, obesity, high-fat diet, MMP-3, MMP11, MMP-12, MMP-13, MMP-14, TIMP-1, TIMP-4, stromal vascular cells, adipogenesis, matrix metalloproteinase inhibitors, gonadal adipose tissue, subcutaneous adipose tissue
- MeSH terms
- Adipose Tissue, Animals, Cell Differentiation, Diet, Enzyme Inhibitors, Epididymis, Gene Expression Regulation, Lung, Male, Obesity, RNA, Messenger, Transcription, Genetic, 3T3 Cells, Adipocytes, Tissue Inhibitor of Metalloproteinases, Reverse Transcriptase Polymerase Chain Reaction, Matrix Metalloproteinases, Mice
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