The DOI matches its Crossref record; 41 of the 41 references it cites were found in a registry or an index.
Ghrelin Directly Interacts With Neuropeptide-Y-Containing Neurons in the Rat Arcuate Nucleus
Open accessAbstract
Ghrelin is a newly discovered peptide that is released from the stomach and from neurons in the hypothalamic arcuate nucleus (ARC) and potently stimulates growth hormone release and food intake. Neuropeptide-Y (NPY) neurons in the ARC play an important role in the stimulation of food intake. The present study aimed to determine whether ghrelin directly activates NPY neurons and, if so, to explore its signaling mechanisms. Whether the neurons that respond to ghrelin could be regulated by orexin and leptin was also examined. We isolated single neurons from the ARC of rats and measured the cytosolic Ca2+ concentration ([Ca2+]i) with fura-2 fluorescence imaging. Ghrelin (10−12 to 10−8 mol/l) concentration-dependently increased [Ca2+]i, which occurred in 35% of the ARC neurons. Approximately 80% of these ghrelin-responsive neurons were proved to be NPY-containing by immunocytochemical staining, and 58% of them were glucose-sensitive neurons as judged by their responses to lowering glucose concentrations. The [Ca2+]i responses to ghrelin were markedly attenuated by inhibitors of protein kinase A (PKA) but not protein kinase C and by a blocker of N-type but not L-type Ca2+ channels. Orexin increased [Ca2+]i and leptin attenuated ghrelin-induced [Ca2+]i increases in the majority (80%) of ghrelin-responsive NPY neurons. These results demonstrate that ghrelin directly interacts with NPY neurons in the ARC to induce Ca2+ signaling via PKA and N-type Ca2+ channel-dependent mechanisms. The integration of stimulatory effects of ghrelin and orexin and inhibitory effect of leptin may play an important role in the regulation of the activity of NPY neurons and thereby feeding.
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Cites
Ghrelin is a growth-hormone-releasing acylated peptide from stomach
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Ghrelin induces adiposity in rodents
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“ICV injection of antighrelin IgG suppresses starvation-induced feeding (3).”
BackgroundA preprandial rise in plasma ghrelin levels suggests a role in meal initiation in humans
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Cited by
Watterson, K. R., Bestow, D., Gallagher, J., Hamilton, D. L., Ashford, F. B., Meakin, P. J., & Ashford, M. L. J. (2013) · Neurosignals · doi:10.1159/000334144 · Cited by 3,389
The role of leptin and ghrelin in the regulation of food intake and body weight in humans: a review
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Biological, Physiological, Pathophysiological, and Pharmacological Aspects of Ghrelin
van der Lely, A. J., Tschöp, M., Heiman, M. L., & Ghigo, E. (2004) · Endocrine Reviews · doi:10.1210/er.2002-0029 · Cited by 1,187
Ghrelin and the short- and long-term regulation of appetite and body weight
Cummings, D. E. (2006) · Physiology & Behavior · doi:10.1016/j.physbeh.2006.05.022 · Cited by 620
The cellular and molecular bases of leptin and ghrelin resistance in obesity
Cui, H., López, M., & Rahmouni, K. (2017) · Nature Reviews Endocrinology · doi:10.1038/nrendo.2016.222 · Cited by 477
Metadata
- Authors
- Kohno, D., Gao, H.-Z., Muroya, S., Kikuyama, S., & Yada, T.
- Venue
- Diabetes
- Publisher
- American Diabetes Association
- Published
- 2003-04-01
- Volume
- 52
- Issue
- 4
- Pages
- 948-956
- ISSN
- 0012-1797 (ISSN-L)
- 1939-327X
- OpenAlex
- W2120228339
- Cited by
- 386
- References
- 41
- FWCI
- 8.63
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Kohno, D., Gao, H.-Z., Muroya, S., Kikuyama, S., & Yada, T. (2003). Ghrelin Directly Interacts With Neuropeptide-Y-Containing Neurons in the Rat Arcuate Nucleus. Diabetes. https://doi.org/10.2337/diabetes.52.4.948
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