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Cited by 74Pancreatic function and diabetesDiabetes, Cardiovascular Risks, and LipoproteinsDiabetes Management and Research

Importance of Obtaining Independent Measures of Insulin Secretion and Insulin Sensitivity During the Same Test

Tripathy, Devjit, Wessman, Ylva, Gullström, Monica, Tuomi, Tiinamaija, Groop, Leif·2003·Diabetes Care
DOI 10.2337/diacare.26.5.1395
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Abstract

OBJECTIVE: To validate and apply a method for independent assessment of insulin secretion and insulin sensitivity (S(I)) during the same test; that is, an intravenous glucose tolerance test followed by a euglycemic-hyperinsulinemic clamp, also called the Botnia clamp. This test was then applied to nondiabetic subjects with (FH+) and without (FH-) a first-degree family history of diabetes. RESEARCH DESIGN AND METHODS: The Botnia clamp measures the first-phase insulin response (FPIR) to 0.3g/kg glucose i.v. and insulin sensitivity (M-value) from a 2-h euglycemic clamp begun 60 min after the glucose bolus. The M-value obtained during the Botnia clamp was compared with M-values obtained during a regular euglycemic clamp without prior glucose bolus. Repeated tests were performed in random order in subjects with normal and abnormal glucose tolerance. Finally, the test was applied to subjects with and without a family history of type 2 diabetes. RESULTS: S(I) and insulin secretion from this test showed a high degree of reproducibility, and the M-value obtained with the Botnia clamp correlated strongly with the M-value from a euglycemic clamp without prior glucose bolus (r = 0.953, P < 0.005). FH+ subjects showed decreased S(I) (P = 0.02), but similar FPIR, compared with FH- subjects. However, insulin secretion adjusted for the degree of insulin resistance was significantly impaired (P = 0.04). CONCLUSIONS: In conclusion, the Botnia clamp provides reliable and independent measures of S(I) and beta-cell function during the same test. As illustrated above, knowledge of the degree of S(I) is mandatory when presenting data on insulin secretion.

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Crossref · 10.2337/diacare.26.5.1395

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27 of 27 references verified0 citation errors · 0 not checkable

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  • Other authors, in validating different types of metabolic challenge tests, have demonstrated the reproducibility of the test results, especially for SI.

    “Other authors, in validating different types of metabolic challenge tests, have demonstrated the reproducibility of the test results, especially for SI (50).”

    From Sleep Restriction for 1 Week Reduces Insulin Sensitivity in Healthy Men (2010) · cites this paper as [50]

  • It is unlikely that the protocol alone (in the absence of sleep restriction) would lead to decreased insulin sensitivity because, in other published studies, repeating these intensive glucose metabolism tests has not led to worsening of metabolic function.

    “It is unlikely that the protocol alone (in the absence of sleep restriction) would lead to decreased insulin sensitivity because, in other published studies, repeating these intensive glucose metabolism test has not led to worsening of metabolic function.”

    From Sleep Restriction for 1 Week Reduces Insulin Sensitivity in Healthy Men (2010) · cites this paper as [50]

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Authors
Tripathy, D., Wessman, Y., Gullström, M., Tuomi, T., & Groop, L.
Affiliations
  • Lund University (SE)
  • Närpes Health Center, Närpes, Finland
  • Helsinki University Hospital (FI)
ORCID
Venue
Diabetes Care
Publisher
American Diabetes Association
Published
2003-05-01
Volume
26
Issue
5
Pages
1395-1401
ISSN
  • 0149-5992 (ISSN-L)
  • 1935-5548
DOI
10.2337/diacare.26.5.1395
OpenAlex
W2030190783
Cited by
74
References
27
Citations by year
2026: 1 · 2025: 3 · 2024: 2 · 2023: 2 · 2022: 3 · 2021: 3 · 2020: 3 · 2019: 2 · 2018: 4 · 2017: 2 · 2016: 4 · 2015: 3 · 2014: 7 · 2013: 3 · 2012: 4
FWCI
2.88
Type
journal article
Language
en
Open access
closed
Locations
  • Diabetes Care (publishedVersion)
  • PubMed (publishedVersion)
  • Lund University Publications (Lund University) (submittedVersion)
Topics
  • Health Sciences › Medicine › Surgery › Pancreatic function and diabetes
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Diabetes, Cardiovascular Risks, and Lipoproteins
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Diabetes Management and Research
Keywords
insulin secretion, insulin sensitivity, first-phase insulin response, hyperinsulinemic-euglycemic clamp, intravenous glucose tolerance test, beta-cell function, insulin resistance, family history of type 2 diabetes, type 2 diabetes risk, test reproducibility
MeSH terms
Insulin Secretion, Diabetes Mellitus, Family, Glucose Tolerance Test, Humans, Hyperinsulinism, Insulin, Reproducibility of Results, Glucose Clamp Technique

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