Changes in the ratio of tetraether to diether lipids in cattle feces in response to altered dietary ratio of grass silage and concentrates1
Open accessAbstract
The distinctive membrane lipids of the archaea can contain a wide range of chemical structures. The membrane lipid composition of ruminal methanogenic archaea has not yet been characterized. In this study, we analyzed proportions of the core archaeal membrane lipids dialkyl glycerol diethers (DGDG) and glycerol dialkyl glycerol tetraether (GDGT). We analyzed the feces of beef steers consuming diets that promoted differences in ruminal conditions that were either favorable (i.e., grass silage) or challenging (i.e., concentrates) for the methanogenic archaea. There was significantly less total ether lipid in the feces of cattle consuming the concentrate diet in comparison to the grass silage diet (97 vs. 218 mg/kg DM, respectively), reflecting the inhibitory effect of dietary concentrate on methanogens. Additionally, the proportion of fecal ether lipids as GDGT was much greater in feces from cattle consuming the concentrate diet than in feces from cattle fed grass silage (90% vs. 67% GDGT). A possible explanation for this adaptation is that membrane lipids composited of GDGT lipids are less permeable to protons, thereby protecting the methanogens against low ruminal pH and helping to maintain the chemiosmotic potential (which is important for ATP production, methanogenesis, and growth). The greater proportion of fecal ether lipids as GDGT may reflect adaptation of membrane lipids within the same species, a shift toward methanogens that have a greater proportion of GDGT (e.g., Thermoplasmata), or both. The effect of ruminal environment on membrane composition means that it will be important to consider the production of both DGDG and GDGT lipids when developing a proxy for methanogenesis.
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Concentrations of the compound archaeol in feces correlate with methane emissions in cattle.
“Nonetheless, concentrations of the compound archaeol in feces has been shown to correlate with methane emissions in cattle (Mccartney et al., 2014).”
From Addressing global ruminant agricultural challenges through understanding the rumen microbiome: past, present, and future (2018) · cites this paper as Mccartney et al., 2014
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- Authors
- McCartney, C. A., Dewhurst, R. J., & Bull, I. D.
- Affiliations
- Teagasc - The Irish Agriculture and Food Development Authority (IE)
- University of Bristol (GB)
- Corresponding author
- McCartney, C. A.
- Venue
- Journal of Animal Science
- Publisher
- Oxford University Press (OUP)
- Published
- 2014-09-01
- Volume
- 92
- Issue
- 9
- Pages
- 4095-4098
- ISSN
- 0021-8812 (ISSN-L)
- 1525-3163
- OpenAlex
- W2111072881
- Cited by
- 7
- References
- 21
- Citations by year
- 2025: 1 · 2019: 1 · 2018: 1 · 2017: 2 · 2015: 1 · 2014: 1
- FWCI
- 2.66
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Animal Science (publishedVersion)
- PubMed (publishedVersion)
- Bristol Research (University of Bristol) (publishedVersion, other-oa)
- Explore Bristol Research (submittedVersion, other-oa)
- Funders
- Biotechnology and Biological Sciences Research Council (1239307)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Agronomy and Crop Science › Ruminant Nutrition and Digestive Physiology
- Physical Sciences › Engineering › Building and Construction › Anaerobic Digestion and Biogas Production
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Nutrition and Physiology
- Keywords
- archaeal membrane lipids, GDGTs, digalactosyldiacylglycerol, ether lipids, cattle feces, grass silage, concentrate diet, rumen pH, methanogenesis, beef steers
- MeSH terms
- Animals, Cattle, Chromatography, High Pressure Liquid, Diet, Feces, Glyceryl Ethers, Poaceae, Lipids, Rumen, Silage, Mass Spectrometry, Molecular Structure, Euryarchaeota
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