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Cited by 10Algal biology and biofuel productionAquaculture Nutrition and GrowthAquatic Ecosystems and Phytoplankton Dynamics

Effects of Temperature and Nitrogen Concentration on Growth and Lipid Accumulation of the Green Algae Chlorella vulgaris for Biodiesel

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Dokuz Eylül Üniversitesi Deniz Bilimleri ve Teknolojisi Enstitüsü, Seyhaneyıldız Can, Şafak, 2 Ege University Fisheries Faculty, Koru, Edis, Ege University Fisheries Faculty, Cirik, Semra, Ege University Fisheries Faculty, Turan, Gamze +4 more·2021·Acta Natura et Scientia
DOI 10.29329/actanatsci.2021.350.03
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Abstract

This study investigated the effect of different temperatures and different nitrogen concentrations on the lipid content and biomass of Chlorella microalgae. In this study, algae were cultured in five media with different amounts as 3, 1.5, 0.80, 0.40 g/L, and three temperatures (10, 20, 30 °C). The results of the experiments showed that the optimal temperature and nitrogen concentration for the biomass increase in Chlorella vulgaris are 30°C and 3 g/L, respectively. It was observed that biomass decreased and lipid amount increased due to the decrease in nitrogen concentration. The high lipid amount of 20.80% dry weight (DW) was obtained from the algae produced at 30°C in the free-nitrate medium. The contribution of temperature change to lipid production was not as effective as nitrogen deficiency in the study. According to the fatty acid analysis results made by GC-FID, C. vulgaris seems suitable for biodiesel production because it contains medium-length (C16-C18) fatty acid chains.

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Research works printed but not linked: 41

  1. Abd El-Baky, H. H., El Baz, F. K., & El-Baroty, G. S. (2004). Production of lipids rich in omega 3 fatty acids from the halotolerant alga Dunaliella salina. Biotechnology, 3(1), 102-108. https://doi.org/10.3923/biotech.2004.102.108 · DOI
  2. Can, Ş. S., Demir, V., & Can, E. (2015). Evaluating the dilution of municipal wastewater on biomass increase, lipid production and nutrient removal by the blue-green algae Spirulina platensis (Geitler). Fresenius Environmental Bulletin, 24(3), 904-909.
  3. Chuck, C. J., Bannister, C. D., Hawley, J. G., Davidson, M. G., La Bruna, I., & Paine, A. (2009). Predictive model to assess the molecular structure of biodiesel fuel. Energy & Fuels, 23(4), 2290-2294. https://doi.org/10.1021/ef801085s · DOI
  4. Converti, A., Casazza, A. A., Ortiz, E. Y., Perego, P., & Del Borghi, M. (2009). Effect of temperature and nitrogen concentration on the growth and lipid content of Nannochloropsis oculata and Chlorella vulgaris for biodiesel production. Chemical Engineering and Processing: Process Intensification, 48(6), 1146-1151. https://doi.org/10.1016/j.cep.2009.03.006 · DOI · Paper page
  5. Deng, X., Li, Y., & Fei, X. (2009). Microalgae: A promising feedstock for biodiesel. African Journal of Microbiology Research, 3(13), 1008-1014.
  6. Dong, H. P., Williams, E., Wang, D. Z., Xie, Z. X., Hsia, R. C., Jenck, A., Halden, R., Li, J., Chen, F., & Place, A. R. (2013). Responses of Nannochloropsis oceanica IMET1 to long-term nitrogen starvation and recovery. Plant Physiology, 162(2), 1110-1126. https://doi.org/10.1104/pp.113.214320 Ekin I. (2019). Quality and composition of lipids used in biodiesel production and methods of transesterification: A review. International Journal of Chemistry and Technology, 3(2), 77-91. https://doi.org/10.32571/ijct.623165 · DOI · Paper page
  7. Griffiths, M. J., & Harrison, S. T. (2009). Lipid productivity as a key characteristic for choosing algal species for biodiesel production. Journal of Applied Phycology, 21(5), 493-507. https://doi.org/10.1007/s10811-008-9392-7 · DOI · Paper page
  8. Guillard, R. R. L. (1975). Culture of phytoplankton for feeding marine invertebrates. In W. L. Smith, & M. H. Chanley (Eds.), Culture of marine invertebrate animals (pp. 29-60). Springer. https://doi.org/10.1007/978-1-4615-8714-9_3 · DOI
  9. Hsieh, C. H., & Wu, W. T. (2009). Cultivation of microalgae for oil production with a cultivation strategy of urea limitation. Bioresource Technology, 100(17), 3921-3926. https://doi.org/10.1016/j.biortech.2009.03.019 · DOI
  10. Illman, A. M., Scragg, A. H., & Shales, S. W. (2000). Increase in Chlorella strains calorific values when grown in low nitrogen medium. Enzyme and Microbial Technology, 27(8), 631-635. https://doi.org/10.1016/s0141-0229(00)00266-0 · DOI · Paper page
  11. Ip, P. F., & Chen, F. (2005). Employment of reactive oxygen species to enhance astaxanthin formation in Chlorella zofingiensis in heterotrophic culture. Process Biochemistry, 40(11), 3491-3496. https://doi.org/10.1016/j.procbio.2005.02.014 · DOI
  12. Lee, S. J., Yoon, B. D., & Oh, H. M. (1998). Rapid method for the determination of lipid from the green alga Botryococcus braunii. Biotechnology Techniques, 12(7), 553-556. https://doi.org/10.1023/A:100881171 · DOI
  13. Li, Q., Du, W., & Liu, D. (2008). Perspectives of microbial oils for biodiesel production. Applied Microbiology and Biotechnology, 80(5), 749-756. https://doi.org/10.1007/s00253-008-1625-9 · DOI
  14. Liew, W. H., Hassim, M. H., & Ng, D. K. (2014). Review of evolution, technology and sustainability assessments of biofuel production. Journal of Cleaner Production, 71, 11-29. https://doi.org/10.1016/j.jclepro.2014.01.006 · DOI
  15. Liu, Z. Y., Wang, G. C., & Zhou, B. C. (2008). Effect of iron on growth and lipid accumulation in Chlorella vulgaris. Bioresource Technology, 99(11), 4717-4722. https://doi.org/10.1016/j.biortech.2007.09.073 · DOI
  16. Lombardi, A. T., & Wangersky, P. J. (1991). Influence of phosphorus and silicon on lipid class production by the marine diatom Chaetoceros gracilis grown in turbidostat cage cultures. Marine Ecology Progress Series, 77, 39-47. https://doi.org/10.3354/meps077039 · DOI
  17. Mandal, S., & Mallick, N. (2009). Microalga Scenedesmus obliquus as a potential source for biodiesel production. Applied Microbiology and Biotechnology, 84(2), 281-291. https://doi.org/10.1007/s00253-009-1935-6 · DOI
  18. Metting, F. B. (1996). Biodiversity and application of microalgae. Journal of Industrial Microbiology, 17(5), 477-489. https://doi.org/10.1007/bf01574779 · DOI
  19. Nigam, S., Rai, M. P., & Sharma, R. (2011). Effect of nitrogen on growth and lipid content of Chlorella pyrenoidosa. American Journal of Biochemistry and Biotechnology, 7(3), 124-129.
  20. Olofsson, M., Lamela, T., Nilsson, E., Bergé, J. P., Del Pino, V., Uronen, P., & Legrand, C. (2014). Combined effects of nitrogen concentration and seasonal changes on the production of lipids in Nannochloropsis oculata. Marine Drugs, 12(4), 1891-1910. https://doi.org/10.3390/md12041891 · DOI
  21. Pal, D., Khozin-Goldberg, I., Cohen, Z., & Boussiba, S. (2011). The effect of light, salinity, and nitrogen availability on lipid production by Nannochloropsis sp. Applied Microbiology and Biotechnology, 90(4), 1429-1441. https://doi.org/10.1007/s00253-011-3170-1 · DOI · Paper page
  22. Park, S. J., Choi, Y. E., Kim, E. J., Park, W. K., Kim, C. W., & Yang, J. W. (2012). Serial optimization of biomass production using microalga Nannochloris oculata and corresponding lipid biosynthesis. Bioprocess and Biosystems Engineering, 35(1), 3-9. https://doi.org/10.1007/s00449-011-0639-3 · DOI
  23. Pinto, E., Sigaud‐kutner, T. C. S., Leitão, M. A., Okamoto, O. K., Morse, D., & Colepicolo, P. (2003). Heavy metal–induced oxidative stress in algae. Journal of Phycology, 39(6), 1008-1018. https://doi.org/10.1111/j.0022-3646.2003.02-193.x · DOI
  24. Renaud, S. M., Thinh, L. V., Lambrinidis, G., & Parry, D. L. (2002). Effect of temperature on growth, chemical composition and fatty acid composition of tropical Australian microalgae grown in batch cultures. Aquaculture, 211(1-4), 195-214. https://doi.org/10.1016/S0044-8486(01)00875-4 · DOI · Paper page
  25. Roleda, M. Y., Slocombe, S. P., Leakey, R. J., Day, J. G., Bell, E. M., & Stanley, M. S. (2013). Effects of temperature and nutrient regimes on biomass and lipid production by six oleaginous microalgae in batch culture employing a two-phase cultivation strategy. Bioresource Technology, 129, 439-449. https://doi.org/10.1016/j.biortech.2012.11.043 · DOI · Paper page
  26. Sajjadi, B., Chen, W. Y., Raman, A. A. A., & Ibrahim, S. (2018). Microalgae lipid and biomass for biofuel production: A comprehensive review on lipid enhancement strategies and their effects on fatty acid composition. Renewable and Sustainable Energy Reviews, 97, 200-232. https://doi.org/10.1016/j.rser.2018.07.050 · DOI · Paper page
  27. Sandnes, J. M., Källqvist, T., Wenner, D., & Gislerød, H. R. (2005). Combined influence of light and temperature on growth rates of Nannochloropsis oceanica: linking cellular responses to large-scale biomass production. Journal of Applied Phycology, 17(6), 515-525. https://doi.org/10.1007/s10811-005-9002-x · DOI
  28. Sissener, N. H., Ørnsrud, R., Sanden, M., Frøyland, L., Remø, S., & Lundebye, A. K. (2018). Erucic acid (22: 1n-9) in fish feed, farmed, and wild fish and seafood products. Nutrients, 10(10), 1443. https://doi.org/10.3390/nu10101443 · DOI
  29. Spolaore, P., Joannis-Cassan, C., Duran, E., & Isambert, A. (2006). Commercial applications of microalgae. Journal of Bioscience and Bioengineering, 101(2), 87-96. https://doi.org/10.1263/jbb.101.87 · DOI · Paper page
  30. Su, C. H., Chien, L. J., Gomes, J., Lin, Y. S., Yu, Y. K., Liou, J. S., & Syu, R. J. (2011). Factors affecting lipid accumulation by Nannochloropsis oculata in a two-stage cultivation process. Journal of Applied Phycology, 23(5), 903-908. https://doi.org/10.1007/s10811-010-9609-4 · DOI
  31. Sun, X., Cao, Y., Xu, H., Liu, Y., Sun, J., Qiao, D., & Cao, Y. (2014). Effect of nitrogen-starvation, light intensity and iron on triacylglyceride/carbohydrate production and fatty acid profile of Neochloris oleoabundans HK-129 by a two-stage process. Bioresource Technology, 155, 204-212. https://doi.org/10.1016/j.biortech.2013.12.109 · DOI
  32. Taoka, Y., Nagano, N., Okita, Y., Izumida, H., Sugimoto, S., & Hayashi, M. (2009). Influences of culture temperature on the growth, lipid content and fatty acid composition of Aurantiochytrium sp. strain mh0186. Marine Biotechnology, 11(3), 368-374. https://doi.org/10.1007/s10126-008-9151-4 · DOI
  33. Van Wagenen, J., Miller, T. W., Hobbs, S., Hook, P., Crowe, B., & Huesemann, M. (2012). Effects of light and temperature on fatty acid production in Nannochloropsis salina. Energies, 5(3), 731-740. https://doi.org/10.3390/en5030731 · DOI
  34. Vasudevan, P. T., & Briggs, M. (2008). Biodiesel production—current state of the art and challenges. Journal of Industrial Microbiology and Biotechnology, 35(5), 421. https://doi.org/10.1007/s10295-008-0312-2 · DOI
  35. Widjaja, A., Chien, C. C., & Ju, Y. H. (2009). Study of increasing lipid production from fresh water microalgae Chlorella vulgaris. Journal of the Taiwan Institute of Chemical Engineers, 40(1), 13-20. https://doi.org/10.1016/j.jtice.2008.07.007 · DOI
  36. Xin, L., Hong-Ying, H., Ke, G., & Ying-Xue, S. (2010). Effects of different nitrogen and phosphorus concentrations on the growth, nutrient uptake, and lipid accumulation of a freshwater microalga Scenedesmus sp. Bioresource Technology, 101(14), 5494-5500. https://doi.org/10.1016/j.biortech.2010.02.016 · DOI · Paper page
  37. Yeh, K. L., & Chang, J. S. (2011). Nitrogen starvation strategies and photobioreactor design for enhancing lipid content and lipid production of a newly isolated microalga Chlorella vulgaris ESP‐31: Implications for biofuels. Biotechnology Journal, 6(11), 1358-1366. https://doi.org/10.1002/biot.201000433 · DOI
  38. Seyhaneyıldız Can, Şafak, Koru, E., Cirik, S., Turan, G., Tekoğul, H., & Subakan, T. (2021). Effects of Temperature and Nitrogen Concentration on Growth and Lipid Accumulation of the Green Algae Chlorella vulgaris for Biodiesel. Acta Natura Et Scientia, 2(2), 101–108. https://doi.org/10.29329/actanatsci.2021.350.03
  39. Acta Natura et Scientia: Vol. 4 No. 2 (2023)
  40. Acta Natura et Scientia: Vol. 2 No. 1 (2021)
  41. Acta Natura et Scientia: Vol. 6 No. 1 (2025) https://prensip.gen.trhttps://prensipjournals.comPublished by Prensip PublishingPublisher Address: Kuzeykent Mah. Orgeneral Atilla Ateş Paşa Cad. No:15 CD, İç Kapı No:31 Merkez, Kastamonu/Türkiye 37150Publisher Contact: info@prensip.gen.trPublisher Technical Support: support@prensipjournals.comJournal Contact: info@actanatsci.com All published content is licensed under a Creative Commons Attribution 4.0 International License.

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Authors
Enstitüsü, D. E. Ü. D. B. V. T., Seyhaneyıldız Can, Ş., 2 Ege University Fisheries Faculty, Koru, E., Ege University Fisheries Faculty, Cirik, S., Ege University Fisheries Faculty, Turan, G., Ege University Fisheries Faculty, Tekoğul, H., Ege University Fisheries Faculty, & Subakan, T.
ORCID
Venue
Acta Natura et Scientia
Publisher
Prensip Publishing
Published
2021-09-21
Volume
2
Issue
2
Pages
101-108
ISSN
2718-0638 (ISSN-L)
DOI
10.29329/actanatsci.2021.350.03
OpenAlex
W4200451833
Cited by
10
References
0
Citations by year
2026: 5 · 2025: 2 · 2023: 2 · 2022: 1
FWCI
0.14
Type
journal article
Language
en
Open access
Licence
cc-by-nc-nd
Locations
  • Acta Natura et Scientia (publishedVersion, cc-by-nc-nd)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
Topics
  • Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
  • Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Aquaculture Nutrition and Growth
  • Physical Sciences › Environmental Science › Environmental Chemistry › Aquatic Ecosystems and Phytoplankton Dynamics
Keywords
Chlorella vulgaris, microalgae, biodiesel, lipid accumulation, nitrogen content, temperature effects, microalgal biomass, fatty acid composition, nitrogen deficiency, culture medium optimization

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