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Cited by 34Neuroinflammation and Neurodegeneration MechanismsTryptophan and brain disordersNeurological Disease Mechanisms and Treatments

Investigation of the optimal dose for experimental lipopolysaccharide-induced recognition memory impairment: behavioral and histological studies

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Alzahrani, Noor Ahmed, Bahaidrah, Khulud Abdullah, Mansouri, Rasha Abdulrashed, Alsufiani, Hadeil Muhanna, Alghamdi, Badrah Saeed·2022·Journal of Integrative Neuroscience
DOI 10.31083/j.jin2102049
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Abstract

Background: Lipopolysaccharide (LPS) administration is one of the most commonly used methods for inducing inflammation in animal models. Several animal studies have investigated the effects of acute and chronic peripheral administration of LPS on cognitive impairment. However, no previous study has compared the effects of different doses of chronically administered LPS on recognition memory performance. Aim: Here, we aimed to investigate the optimal dose of chronically administered LPS for the induction of recognition memory impairment in mice. Materials and methods: LPS at different doses (0.25, 0.50 and 0.75 mg/kg) was administered to SWR/J mice daily for 7 days. On day 9, the open field, novel object recognition and novel arm discrimination behavioral tests were performed. Additionally, prefrontal cortical histological examination was conducted. Results: Compared with the control group, mice injected with 0.75 mg/kg LPS notably showed no object preference (familiar vs. novel), a reduction in the discrimination index, and spatial recognition impairment. Administration of the 0.25 and 0.50 mg/kg doses of LPS showed a preference for the novel object compared with the familiar object, had no significant impact on the discrimination index, and caused spatial recognition impairment. These behavioral results are in line with the histological examination of the prefrontal cortex, which revealed that the 0.75 mg/kg dose produced the most histological damage. Conclusions: Our findings suggest that for chronic peripheral administration of LPS, 0.75 mg/kg is the optimal dose for inducing neuroinflammation-associated recognition memory deficits.

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Cited by

Sorted by citations

Authors
Alzahrani, N. A., Bahaidrah, K. A., Mansouri, R. A., Alsufiani, H. M., & Alghamdi, B. S.
Affiliations
King Abdulaziz University (SA)
Corresponding author
Alzahrani, Noor Ahmed
ORCID
Venue
Journal of Integrative Neuroscience
Publisher
IMR Press
Published
2022-03-21
Volume
21
Issue
2
Pages
49-49
ISSN
  • 0219-6352 (ISSN-L)
  • 1757-448X
DOI
10.31083/j.jin2102049
OpenAlex
W4220661307
Cited by
34
References
4
Citations by year
2026: 12 · 2025: 6 · 2024: 10 · 2023: 5 · 2022: 1
FWCI
1.91
Type
journal article
Language
en
Open access
Licence
Locations
  • Journal of Integrative Neuroscience (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Topics
  • Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
  • Life Sciences › Neuroscience › Biological Psychiatry › Tryptophan and brain disorders
  • Life Sciences › Neuroscience › Neurology › Neurological Disease Mechanisms and Treatments
Keywords
lipopolysaccharide, dose optimization, recognition memory impairment, neuroinflammation, novel object recognition, spatial recognition, cognitive impairment, mouse model
MeSH terms
Animals, Inflammation, Lipopolysaccharides, Memory Disorders, Recognition, Psychology, Mice, Cognitive Dysfunction

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