Cited by 523D Printing in Biomedical ResearchCellular Mechanics and InteractionsMicrofluidic and Capillary Electrophoresis Applications

Whole-cell biochips for bio-sensing: integration of live cells and inanimate surfaces

Ben-Yoav, Hadar, Melamed, Sahar, Freeman, Amihay, Shacham-Diamand, Yosi, Belkin, Shimshon·2011·Critical Reviews in Biotechnology

Abstract

Recent advances in the convergence of the biological, chemical, physical, and engineering sciences have opened new avenues of research into the interfacing of diverse biological moieties with inanimate platforms. A main aspect of this field, the integration of live cells with micro-machined platforms for high throughput and bio-sensing applications, is the subject of the present review. These unique hybrid systems are configured in a manner that ensures positioning of the cells in designated patterns, and enables cellular viability maintenance, and monitoring of cellular functionality. Here we review both animate and inanimate surface properties and how they affect cellular attachment, describe relevant modifications of both types of surfaces, list technologies for platform engineering and for cell deposition in the desired configurations, and discuss the influence of various deposition and immobilization methods on the viability and performance of the immobilized cells.

Integrity signals

Registry

Crossref · 10.3109/07388551.2010.532767

Cites

213 of 213 references verified0 citation errors · 0 not checkable

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  • Cells grown in 3D culture can be incorporated into a biosensor either by direct attachment onto a biotic or abiotic substrate surface or by indirect attachment via entrapment in a biocompatible biopolymer.

    “Cells grown in 3D culture can be incorporated into a biosensor either by direct attachment onto a biotic or abiotic substrate surface or by indirect attachment via entrapment in a biocompatible biopolymer. 83,84”
  • Silicon offers advantageous electrical and mechanical properties but lacks optical transparency, limiting the utility of luminescent and fluorescent analytical techniques.

    “Silicon offers advantageous electrical and mechanical properties but lacks optical transparency, thus limiting the utility of luminescent and fluorescent analytical techniques. 83”
  • Plastics are inexpensive but autofluorescent and tend to take up water and other solvents, attributes that can skew fluorescence signals from the sensing element.

    “Plastics, although inexpensive, are autofluorescent and have a tendency to take up water and other solvents-attributes that can skew fluorescence signals from the sensing element. 83”

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Authors
Ben-Yoav, H., Melamed, S., Freeman, A., Shacham-Diamand, Y., & Belkin, S.
Affiliations
  • Hebrew University of Jerusalem (IL)
  • Tel Aviv University (IL)
Venue
Critical Reviews in Biotechnology
Published
2010-12-30
Volume
31
Issue
4
Pages
337-353
ISSN
  • 0738-8551 (ISSN-L)
  • 1549-7801
OpenAlex
W1969803714
Cited by
52
References
214
Citations by year
2025: 2 · 2024: 1 · 2023: 1 · 2022: 1 · 2021: 1 · 2020: 3 · 2019: 6 · 2018: 1 · 2017: 4 · 2016: 2 · 2015: 4 · 2014: 8 · 2013: 7 · 2012: 7
FWCI
0.47
Type
journal article
Language
en
Open access
closed
Locations
  • Critical Reviews in Biotechnology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
  • Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Capillary Electrophoresis Applications
Keywords
biosensing, cell patterning, cell-surface interactions, surface modification, cell viability
MeSH terms
Bacteria, Cell Adhesion, Cell Survival, Surface Properties, Biosensing Techniques, Cells, Immobilized, Microfluidic Analytical Techniques, Microarray Analysis

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