Whole-cell biochips for bio-sensing: integration of live cells and inanimate surfaces
Abstract
Recent advances in the convergence of the biological, chemical, physical, and engineering sciences have opened new avenues of research into the interfacing of diverse biological moieties with inanimate platforms. A main aspect of this field, the integration of live cells with micro-machined platforms for high throughput and bio-sensing applications, is the subject of the present review. These unique hybrid systems are configured in a manner that ensures positioning of the cells in designated patterns, and enables cellular viability maintenance, and monitoring of cellular functionality. Here we review both animate and inanimate surface properties and how they affect cellular attachment, describe relevant modifications of both types of surfaces, list technologies for platform engineering and for cell deposition in the desired configurations, and discuss the influence of various deposition and immobilization methods on the viability and performance of the immobilized cells.
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Cells grown in 3D culture can be incorporated into a biosensor either by direct attachment onto a biotic or abiotic substrate surface or by indirect attachment via entrapment in a biocompatible biopolymer.
“Cells grown in 3D culture can be incorporated into a biosensor either by direct attachment onto a biotic or abiotic substrate surface or by indirect attachment via entrapment in a biocompatible biopolymer. 83,84”
From Three-dimensional cell culture systems and their applications in drug discovery and cell-based biosensors (2014) · cites this paper as [83]
Silicon offers advantageous electrical and mechanical properties but lacks optical transparency, limiting the utility of luminescent and fluorescent analytical techniques.
“Silicon offers advantageous electrical and mechanical properties but lacks optical transparency, thus limiting the utility of luminescent and fluorescent analytical techniques. 83”
From Three-dimensional cell culture systems and their applications in drug discovery and cell-based biosensors (2014) · cites this paper as [83]
Plastics are inexpensive but autofluorescent and tend to take up water and other solvents, attributes that can skew fluorescence signals from the sensing element.
“Plastics, although inexpensive, are autofluorescent and have a tendency to take up water and other solvents-attributes that can skew fluorescence signals from the sensing element. 83”
From Three-dimensional cell culture systems and their applications in drug discovery and cell-based biosensors (2014) · cites this paper as [83]
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- Authors
- Ben-Yoav, H., Melamed, S., Freeman, A., Shacham-Diamand, Y., & Belkin, S.
- Affiliations
- Hebrew University of Jerusalem (IL)
- Tel Aviv University (IL)
- Venue
- Critical Reviews in Biotechnology
- Publisher
- Informa UK Limited
- Published
- 2010-12-30
- Volume
- 31
- Issue
- 4
- Pages
- 337-353
- ISSN
- 0738-8551 (ISSN-L)
- 1549-7801
- OpenAlex
- W1969803714
- Cited by
- 52
- References
- 214
- Citations by year
- 2025: 2 · 2024: 1 · 2023: 1 · 2022: 1 · 2021: 1 · 2020: 3 · 2019: 6 · 2018: 1 · 2017: 4 · 2016: 2 · 2015: 4 · 2014: 8 · 2013: 7 · 2012: 7
- FWCI
- 0.47
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Critical Reviews in Biotechnology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Capillary Electrophoresis Applications
- Keywords
- biosensing, cell patterning, cell-surface interactions, surface modification, cell viability
- MeSH terms
- Bacteria, Cell Adhesion, Cell Survival, Surface Properties, Biosensing Techniques, Cells, Immobilized, Microfluidic Analytical Techniques, Microarray Analysis
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