Interaction between combustion-generated organic nanoparticles and biological systems:In vitro study of cell toxicity and apoptosis in human keratinocytes
Abstract
The purpose of this work was to evaluate the effect of flame-generated nucleation mode particles with an organic carbon structure on growth and apoptosis in immortalized human keratinocytes. In this study, cells were stimulated with nanoparticles collected from flames that produce only nucleation mode particles operating with a fuel:air mixture typical of low-emission combustion systems. Cytotoxicity as a function of particle concentration was monitored by fluorescence-activated cell sorting (FACS) analysis, and apoptosis was observed by FACS using DNA fragmentation and hypodiploidism and confirmed by annexin assay. A dose-dependent reduction in cell viability by apoptosis in incubation periods of 48 and 72 hours was observed with a statistically significant increase in apoptosis over controls for a dose larger than 7 μg/mL (1.4 μg/cm²). The results presented here may be relevant for understanding the association between exposure to traffic-generated particulate pollution and enhanced skin aging reported in epidemiology studies.
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Free radicals and organic nanoparticles reduced human keratinocyte viability by apoptosis.
“free radicals and organic nanoparticles reduced human keratinocyte viability by apoptosis [43,45,46]”
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- Authors
- Pedata, P., Boccellino, M., La Porta, R., Napolitano, M., Minutolo, P., Sgro, L. A., Zei, F., Sannolo, N., & Quagliuolo, L.
- Affiliations
- University of Campania "Luigi Vanvitelli" (IT)
- Istituto Nazionale Tumori IRCCS "Fondazione G. Pascale" (IT)
- Institute for Research on Combustion (IT)
- University of Naples Federico II (IT)
- Corresponding author
- Minutolo, Patrizia
- Venue
- Nanotoxicology
- Publisher
- Informa UK Limited
- Published
- 2011-05-16
- Volume
- 6
- Issue
- 4
- Pages
- 338-352
- ISSN
- 1743-5390 (ISSN-L)
- 1743-5404
- OpenAlex
- W2008790615
- Cited by
- 31
- References
- 69
- Citations by year
- 2025: 1 · 2024: 1 · 2023: 1 · 2022: 3 · 2021: 2 · 2020: 2 · 2019: 6 · 2018: 1 · 2017: 1 · 2016: 2 · 2015: 4 · 2014: 1 · 2013: 2 · 2012: 3
- FWCI
- 1.62
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Nanotoxicology (publishedVersion)
- PubMed (publishedVersion)
- Archivio Istituzionale della Ricerca - Università degli Studi della Campania "Luigi Vanvitelli" (submittedVersion)
- Topics
- Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Air Quality and Health Impacts
- Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Toxic Organic Pollutants Impact
- Physical Sciences › Materials Science › Materials Chemistry › Nanoparticles: synthesis and applications
- Keywords
- nucleation mode particles, keratinocytes, apoptosis, cytotoxicity, DNA fragmentation, traffic-related particulate matter, skin aging, low-emission combustion
- MeSH terms
- Carbon, Cell Line, DNA, Humans, Spectrometry, Fluorescence, Keratinocytes, Apoptosis, Toxicity Tests, Particulate Matter, Nanoparticles
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