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Susceptibility Rhythm to E. coli Endotoxin and Bioassay.
Abstract
Light-synchronized periodicity analysis reveals a susceptibility rhythm in C mice to E. coli endotoxin. Susceptibility varies predictably and significantly along the 24-hour time scale. A dose of endotoxin which is compatible with survival of most animals when given during middle of daily dark period is highly lethal when it is given 8-12 hours earlier or later. LD50 also was determined for 2 samples from identical batch of endotoxin tested 12 h apart on separate groups of comparable C mice kept under conditions standardized for periodicity analysis: Differences in potency significant at 1% were seen at 2 dose levels common to both assays, the computed “potency ratio” being 3.22.
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“…mice injected with LPS at the end of the day (ZT11, where ZT0 marks light onset and ZT12 light offset) exhibit a mortality rate of approximately 80%, whereas those injected in the middle of the night (ZT19) have a mortality rate of about 30% (Halberg et al., 1960; Marpegan et al., 2009).”
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“Significant susceptibility rhythms have been also found for harmful agents, such as audiogenic stimulation (Halbert et al., 1955), E. coli endotoxin (Halberg et al., 1960), ethanol, ouabain (Nelson and Halberg, 1973), potassium cyanide (Bafitis et al., 1978) and chemical carcinogens (Iverson et…”
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“This was substantiated experimentally in mice by Halberg et al., who reported that mice exposed to LPS in the active vs rest phase had a statistically significant difference in observed mortality [45,46].”
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Metadata
- Authors
- Halberg, F., Johnson, E. A., Brown, B. W., & Bittner, J. J.
- Venue
- Experimental Biology and Medicine
- Publisher
- Frontiers Media SA
- Published
- 1960-01-01
- Volume
- 103
- Issue
- 1
- Pages
- 142-144
- ISSN
- 0071-3384 (ISSN-L)
- 1535-3702
- 1535-3699
- OpenAlex
- W2321428190
- Cited by
- 280
- References
- 0
- Citations by year
- 2026: 8 · 2025: 15 · 2024: 21 · 2023: 16 · 2022: 12 · 2021: 13 · 2020: 19 · 2019: 14 · 2018: 9 · 2017: 14 · 2016: 12 · 2015: 8 · 2014: 11 · 2013: 11 · 2012: 11
- FWCI
- 3.25
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Experimental Biology and Medicine (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Circadian rhythm and melatonin
- Health Sciences › Medicine › Critical Care and Intensive Care Medicine › Sepsis Diagnosis and Treatment
- Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
- Keywords
- endotoxin, Escherichia coli, circadian rhythm, periodicity analysis, light synchronization, mice, LD50, dose-response relationship, time of administration
- MeSH terms
- Biological Assay, Endotoxins, Escherichia coli, Humans
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