MicroRNA profile in patients with Alzheimer’s disease: analysis of miR-9-5p and miR-598 in raw and exosome enriched cerebrospinal fluid samples
Abstract
Background MicroRNAs have been postulated as potential biomarkers for Alzheimer's disease (AD). Exosomes are nanovesicles which transport microRNAs, proteins, and other cargos. It has been hypothesized that the exosome traffic might be increased in neurodegenerative disorders. Objective i) To assess the cerebrospinal fluid (CSF) microRNA profile in a group of AD patients and control subjects and to validate a group of microRNAs previously reported by other authors. ii) To compare microRNA levels in whole CSF and in the exosome-enriched fraction in AD patients. Methods A panel of 760 microRNAs was analyzed in the CSF of 10 AD patients and 10 healthy subjects. Among microRNAs differently expressed, we selected those that had been previously reported by other authors. Candidates were validated in a larger group by individual qPCR assays. MicroRNA expression was also evaluated in exosome-enriched CSF samples of patients with AD and controls. Results Fifteen microRNAs were differently expressed in AD. MiR-9-5p, miR-134, and miR-598 were selected as candidates for further analysis. MiR-9-5p and miR-598 were detected in 50 and 75% of control CSF samples, respectively, while they were not detected in any AD CSF samples. We observed an opposite pattern when we evaluated the microRNA expression in the exosome-enriched CSF AD samples. No pattern variations were noted among healthy subjects. Conclusion These data propose miR-9-5p and miR-598 as potential biomarkers for AD. Further studie…
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miR-9-5p and miR-598 are depleted in whole CSF from AD patients but enriched in exosome-enriched CSF fractions.
“In one study, Riancho and colleagues demonstrated that miR-9-5p and miR-598 are depleted in whole CSF from AD patients but enriched in exosome-enriched CSF fractions, underscoring how EV trafficking and subfraction choice can profoundly influence biomarker readouts (Riancho et al., 2017).”
From Cell-specific MicroRNA networks orchestrate the pathogenesis of Alzheimer’s disease (2026) · cites this paper as Riancho et al., 2017
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MicroRNA Profiling of CSF Reveals Potential Biomarkers to Detect Alzheimer`s Disease
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Cell-specific MicroRNA networks orchestrate the pathogenesis of Alzheimer’s disease
Donepudi, K., Eadha, S., Rodarte, D., Sharma, B., & Kumar, S. (2026) · Ageing Research Reviews · doi:10.1016/j.arr.2026.103211
- Authors
- Riancho, J., Vázquez-Higuera, J. L., Pozueta, A., Lage, C., Kazimierczak, M., Bravo, M., Calero, M., Gonalezález, A., Rodríguez, E., Lleó, A., & Sánchez-Juan, P.
- Affiliations
- Service of Neurology, University Hospital Marqués de Valdecilla –IDIVAL; CIBERNED, Santander, Spain
- CIBERNED; National Institute of Health Carlos III; Alzheimer Disease Research Unit, CIEN Foundation; Queen Sofia Foundation Alzheimer Center; Chronic Disease Programme Carlos III Institute of Health, Madrid, Spain
- Department of Neurology, Memory Unit, Hospital de la Santa Creu i Sant Pau, Biomedical Research Institute Sant Pau, Universitat Autònoma de Barcelona, Barcelona, Spain; CIBERNED.
- Venue
- Journal of Alzheimer's Disease
- Publisher
- SAGE Publications
- Published
- 2017-03-21
- Volume
- 57
- Issue
- 2
- Pages
- 483-491
- ISSN
- 1387-2877
- 1875-8908
- Cited by
- 143
- References
- 4
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