In vitro regeneration and PCR-RAPD based detection of somaclonal variation in kenaf (Hibiscus cannabinus)
Open accessAbstract
Though direct systems of regeneration through culture of organized meristems usually produce true-to-type plants, variations in the progenies have widely been reported. Fiber producing kenaf plants (Hibiscus cannabinus L.) were regenerated from petiole, hypocotyls and cotyledonous petiole explants on MS medium containing BAP (benzyl amino purine) and NAA (?-napthaleneacetic acid) followed by assessment of regenerants by RAPD markers to detect somaclonal variation among them. Genomic DNA from twenty seven plants [three mother plants and two clones (clone 1 and 2) from each mother plant with three replications] was subjected to random amplified polymorphic DNA (RAPD) analysis. Fifteen polymorphic loci amplified by three decamer random primers were used to estimate genetic diversity and relatedness in mother plants and their regenerated plantlets. The results showed some degree of polymorphism between mother plants and their regenerated plantlets as well as between regenerated plantlets indicating somaclonal variation among the regenerants. These suggest that the RAPD technique could effectively be used to detect somaclonal variation in H. cannabinus and could be promising for the detection of markers associated with desirable traits.Progressive Agriculture 28 (2): 100-108, 2017
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In Hibiscus cannabinus, 3 of 25 decametric primers were selected for analysis of the whole sample set.
“Haque et al. (2017) [28], working on Hibiscus cannabinus L., screened 25 decametric primers and selected only 3 for the analysis of the whole sample set.”
From Somaclonal Variation—Advantage or Disadvantage in Micropropagation of the Medicinal Plants (2023) · cites this paper as [28]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures
Murashige, T., & Skoog, F. K. (1962) · Physiologia Plantarum · doi:10.1111/j.1399-3054.1962.tb08052.x · Cited by 67,420 · see citing works
DNA polymorphisms amplified by arbitrary primers are useful as genetic markers
Williams, J. G., Kubelik, A. R., Livak, K. J., Rafalski, J. A., & Tingey, S. V. (1990) · Nucleic Acids Research · doi:10.1093/nar/18.22.6531 · Cited by 13,077 · see citing works
Analysis of Gene Diversity in Subdivided Populations
Nei, M. (1973) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.70.12.3321 · Cited by 8,885 · see citing works
Somaclonal variation — a novel source of variability from cell cultures for plant improvement
Larkin, P. J., & Scowcroft, W. R. (1981) · Theoretical and Applied Genetics · doi:10.1007/bf02342540 · Cited by 3,002 · see citing works
The similarity index and DNA fingerprinting.
Lynch, M. F. (1990) · Molecular Biology and Evolution · doi:10.1093/oxfordjournals.molbev.a040620 · Cited by 643 · see citing works
Genetic instability of plant tissue cultures: breakdown of normal controls.
Phillips, R. L., Kaeppler, S. M., & Olhoft, P. M. (1994) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.91.12.5222 · Cited by 596 · see citing works
Analysis of Population Genetic Structure by DNA Fingerprinting
Lynch, M. (1991) · Proceedings of the Fourth International Symposium on Polarization Phenomena in Nuclear Reactions · doi:10.1007/978-3-0348-7312-3_9 · Cited by 213 · see citing works
Sources and Frequency of Somaclonal Variation
Skirvin, R. M., McPheeters, K. D., & Norton, M. A. (1994) · HortScience · doi:10.21273/hortsci.29.11.1232 · Cited by 212 · see citing works
Plant Responses to Environmental Stresses: From Phytohormones to Genome Reorganization
Tudzynski, B. (2001) · Phytochemistry · doi:10.1016/s0031-9422(00)00375-7 · Cited by 209 · see citing works
Epigenetic aspects of somaclonal variation in plants
Kaeppler, S. M., Kaeppler, H. F., & Rhee, Y. (2000) · Springer eBooks · doi:10.1007/978-94-011-4183-3_4 · Cited by 108 · see citing works
Phenotype and cytological status of plants regenerated from callus cultures of Allium sativum L.
Novák, F. (1980) · Cited by 60 · see citing works
DNA Fingerprinting of Jute Germplasm by RAPD
Hossain, M. B., Haque, S., & Khan, H. (2002) · BMB Reports · doi:10.5483/bmbrep.2002.35.4.414 · Cited by 56 · see citing works
Self-citationCHENG, Z., Lu, B., Baldwin, B. S., 鮫島, 一., & Chen, J. (2002) · Hereditas · doi:10.1034/j.1601-5223.2002.1360309.x · Cited by 45 · see citing works
Genetic polymorphism in rice ( Oryza sativa L.) through RAPD analysis
Rahman, S. N., Islam, M. S., Alam, M. S., & Nasiruddin, K. M. (2007) · Cited by 32 · see citing works
Self-citationCallus induction and adventitious organogenesis of kenaf (Hibiscus cannabinus L.)
McLean, K. S., Lawrence, K. S., & Reichert, N. A. (1992) · Plant Cell Reports · doi:10.1007/bf00236272 · Cited by 27 · see citing works
Analysis of Genetic Diversity in Bangladeshi Chicken using RAPD Markers
Mollah, M. B. R., Islam, F. B., Islam, M. S., Ali, M. A. M., & Alam, M. S. (2009) · Biotechnology(Faisalabad) · doi:10.3923/biotech.2009.462.467 · Cited by 25 · see citing works
Self-citationMaharana, S. M., Mahato, V., Behera, M., Mishra, R. R., & Panigrahi, J. (2012) · Indian Journal of Biotechnology · Cited by 19 · see citing works
Bala, B., Mallik, M., Saclain, S., & Islam, M. S. (2017) · Journal of Genetic Engineering and Biotechnology · doi:10.1016/j.jgeb.2017.02.006 · Cited by 14 · see citing works
Self-citationGenetic and Epigenetic Instability in Tissue Culture and Regenerated Progenies
Olhoft, P. M., & Phillips, R. L. (2018) · Routledge eBooks · doi:10.1201/9780203743157-7 · Cited by 11 · see citing works
Saclain, S., Latif, A., Bala, B., Mallik, M., & Islam, M. S. (2016) · Genetika · doi:10.2298/gensr1601151s · Cited by 6 · see citing works
Self-citation
Flagged first, then by citations
Research works printed but not linked: 5
- References AKS, Proceedings of the 3rd annual American kenaf society conference held on 23-25 February 2000 (American kenaf society corpus christi 2 TX, USA).
- Hanboonsong Y, Vinijsanun T, & Ponragdee W, Molecular characterization and genetic relationships of roselle germplasm in Thailand, in Application of biotechnology in the improvement of jute, kenaf and allied fibres-phase II, (IJO/ AGR/10), final workshop, held on10-12 August 2000, Beijing, China, 2000 95-106.
- JKA, Proceedings of the international kenaf symposium, held on 13-14 October 2000 (Japan Kenaf Association, Hiroshima, Japan).
- Li Z D (1980). Kenaf. In: (ed.), Theory and technology of fiber crops (in Chinese). Scientific and Technological Press, Shanghai. pp. 541-595.
- Michelmore, R.W., Paran, I., and Kesseli, R. Y. (1991). Identification of markers linked to disease resistance genes by bulked segregant analysis: A rapid method to detect markers in specific genomic regions by using segregating population. Proc. Natl. Acad. Sci. USA. 88: 9828-9832.
Cited by
Sorted by citations
Somaclonal Variation—Advantage or Disadvantage in Micropropagation of the Medicinal Plants
Duta-Cornescu, G., Constantin, N., Pojoga, D.-M., Nicuta, D., & Simon-Gruita, A. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms24010838 · Cited by 108 · see citing works
“18% polymorphism between mother plant and micropropagated plants - the decrease in NAA and the increase in BAP increases the percentage of polymorphism [28]”
BackgroundCharacteristics of Kenaf (Hibiscus cannabinus L.) Mutants Induced by Gamma-Ray
Lee, I.-S., Kang, C.-H., Kwon, S.-J., & Na, Y. (2019) · Journal of Agricultural Science and Technology B · doi:10.17265/2161-6264/2019.04.003 · Cited by 1 · see citing works
Saavedra-García, J. M., Hernández-Amasifuen, Á. D., Pineda-Lázaro, A. J., Delgado-Mera, E., Peláez-Rivera, J. L., ASCÓN-DIONICIO, G. U., Ríos-Ramírez, O., Flores, E. T., Sánchez, M. E. R., & Corazón-Guivin, M. A. (2026) · Plants · doi:10.3390/plants15152327
- Authors
- Haque, M., Biswas, T., Islam, M., & Hossain, M.
- Affiliations
- Bangladesh Agricultural University (BD)
- Bangladesh Jute Research Institute (BD)
- ORCID
- Biswas, T
- Venue
- Progressive Agriculture
- Publisher
- Bangladesh Journals Online (JOL)
- Published
- 2017-08-09
- Volume
- 28
- Issue
- 2
- Pages
- 100-108
- ISSN
- 1017-8139 (ISSN-L)
- 2310-2950
- OpenAlex
- W2742498160
- Cited by
- 3
- References
- 20
- Citations by year
- 2026: 1 · 2023: 1 · 2019: 1
- FWCI
- 0.11
- Type
- journal article
- Language
- en
- Open access
- diamond
- doi.org
- Locations
- Progressive Agriculture (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Genetic and Mutation Studies
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Research in Cotton Cultivation
- Keywords
- kenaf, somaclonal variation, in vitro regeneration, RAPD markers, genetic diversity, plant tissue culture, benzylaminopurine, hypocotyl explants, petiole explants, marker-assisted selection
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
