The MicroRNA-23a Has Limited Roles in Bone Formation and Homeostasis In Vivo
Open accessAbstract
(miRNAs) inhibit bone formation by inhibiting the translation of specific genes. Several in vitro studies have suggested that miR-23a inhibits osteogenic differentiation by suppressing the translation of Runx2, a transcription factor essential for osteoblastogenesis, and of Satb2, a member of the special AT rich binding protein family. In the present study, we used a gain-of-function approach to determine the roles of miR-23a in bone formation and homeostasis in vivo. The miR-23a transgenic (Tg) mice grew normally and their body size and weight were similar to those of wild-type (WT) littermates. Bone structure and morphology were similar in Tg and WT mice. Furthermore, the numbers of osteoblasts and osteoclasts, as well as their activities in bone were similar between Tg and WT mice. Our results indicate that miR-23 has limited roles in bone formation and maintenance in vivo in mice.
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miR-23a is highly expressed in osteoblasts, chondrocytes, and fibroblast cell lines.
“miR-23a is highly expressed in osteoblasts, chondrocytes, and fibroblast cell lines.”
From Recent advances of miR-23 in human diseases and growth development (2025) · cites this paper as [110]
The Discussion reports induction of miR-23a in MC3T3-E1 cells, an in vitro model of osteogenic differentiation, and the Tissue expression section reports higher expression in bone than in several other tissues. Neither passage establishes high expression specifically in osteoblasts, chondrocytes, and fibroblast cell lines; the copy does not report expression in the latter two cell types. (LLM, conf=0.77)
Experimental — accuracy not yet measuredIn vitro experiments demonstrated that miR-23a can control the progression of osteoblast and chondrocyte lineages and inhibit osteogenic differentiation by targeting Runx2 and Satb2 in mice.
“The experiments in vitro have demonstrated that it can control the progression of osteoblast and chondrocyte lineages and inhibit osteogenic differentiation by targeting the transcription factors Runx2 and Satb2 in mice.”
From Recent advances of miR-23 in human diseases and growth development (2025) · cites this paper as [110]
The Discussion reports that miR-23a overexpression inhibited osteogenic differentiation in cultured MC3T3-E1 cells by suppressing Runx2 and Satb2 translation. Our copy does not establish that miR-23a controls chondrocyte lineage progression. It also reports no comparable effect on osteogenic differentiation in the transgenic mice, so the in vitro finding should not be read as an in vivo result. The supplied passages have section labels but no page numbers. (LLM, conf=0.86)
Experimental — accuracy not yet measuredThere existed no notable variance in the number of osteoblasts and osteoclasts between miR-23 transgenic and wild-type mice, along with their activity in bone.
“there existed no notable variance in the number of osteoblasts and osteoclasts between miR-23 transgenic and wild-type mice, along with their activity in the bone.”
From Recent advances of miR-23 in human diseases and growth development (2025) · cites this paper as [110]
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- Authors
- PARK, J., WADA, S., USHIDA, T., & AKIMOTO, T.
- Affiliations
- Konkuk University (KR)
- Center for Disease Biology and Integrative Medicine, The University of Tokyo (JP)
- The University of Tokyo (JP)
- Venue
- Physiological Research
- Published
- 2015-10-16
- Volume
- 64
- Issue
- 5
- Pages
- 711-719
- ISSN
- 0862-8408 (ISSN-L)
- 1802-9973
- OpenAlex
- W310092238
- Cited by
- 14
- References
- 34
- Citations by year
- 2024: 1 · 2023: 1 · 2022: 2 · 2021: 1 · 2020: 2 · 2019: 3 · 2017: 2 · 2015: 2
- FWCI
- 0.58
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by-nc
- Locations
- Physiological Research (publishedVersion, cc-by-nc)
- PubMed (publishedVersion)
- Funders
- Ministry of Education, Culture, Sports, Science and Technology (21680049)
- University of Tokyo
- Japan Society for the Promotion of Science (21680049, 22240058, 25282198)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
- Health Sciences › Medicine › Orthopedics and Sports Medicine › Bone health and osteoporosis research
- Keywords
- miR-23a, microRNA, bone formation, bone homeostasis, osteoblasts, osteoclasts, Runx2, SATB2, osteogenic differentiation, transgenic mice, gain-of-function
- MeSH terms
- Animals, Homeostasis, Male, Mice, Inbred C57BL, Mice, Transgenic, Osteoblasts, Osteogenesis, MicroRNAs, Mice
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