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Cited by 25biodegradable polymer synthesis and propertiesPolymer Surface Interaction StudiesCoronary Interventions and Diagnostics

Cytocompatibility Evaluation of a Novel Series of PEG-Functionalized Lactide-Caprolactone Copolymer Biomaterials for Cardiovascular Applications

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Pacharra, Sandra, McMahon, Seán, Duffy, Patrick, Basnett, Pooja, Yu, Wenfa, Seisel, Sabine, Stervbo, Ulrik, Babel, Nina +4 more·2020·Frontiers in Bioengineering and Biotechnology
DOI 10.3389/fbioe.2020.00991PMC PMC7438451
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Abstract

Although the use of bioresorbable materials in stent production is thought to improve long-term safety compared to their durable counterparts, a recent FDA report on the two-year follow-up of the first FDA-approved bioresorbable vascular stent showed an increased occurrence of major adverse cardiac events and thrombosis in comparison to the metallic control. In order to overcome the issues of first generation bioresorbable polymers, a series of polyethylene glycol-functionalized poly-L-lactide-co-ɛ-caprolactone copolymers with varying lactide-to-caprolactone content is developed using a novel one-step PEG-functionalization and copolymerization strategy. This approach represents a new facile way towards surface enhancement for cellular interaction, which is shown by screening these materials regarding their cyto- and hemocompatibility in terms of cytotoxicity, hemolysis, platelet adhesion, leucocyte activation and endothelial cell adhesion. By varying the lactide-to-caprolactone polymer composition, it is possible to gradually affect endothelial and platelet adhesion which allows fine-tuning of the biological response based on polymer chemistry. All polymers developed were non-cytotoxic, had acceptable leucocyte activation levels and presented non-hemolytic (< 2% hemolysis rate) behavior except for PLCL-PEG 55:45 which presented hemolysis rate of 2.5%±0.5. Water contact angles were reduced in the polymers containing PEG functionalization (PLLA-PEG: 69.8°±2.3, PCL-PEG: 61.2°±7.5) versus those without (PLLA: 79.5°±3.2, PCL: 76.4°±10.2) while the materials PCL-PEG550, PLCL-PEG550 90:10 and PLCL-PEG550 70:30 demonstrated best endothelial cell adhesion. PLLA-PEG550 and PLCL-PEG550 70:30 presented as best candidates for cardiovascular implant use from a cytocompatibility perspective across the spectrum of testing completed. Altogether, these polymers are excellent innovative materials suited for an application in stent manufacture due to the ease in translation of this one-step synthesis strategy to device production and their excellent in vitro cyto- and hemocompatibility.

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  • PEG-functionalized lactide-caprolactone copolymers demonstrated improved endothelial cytocompatibility and hemocompatibility in vitro.

    “For example, PEG-functionalized lactide–caprolactone copolymers demonstrated improved endothelial cytocompatibility and hemocompatibility in vitro [199].”

    From Biomaterials in Postoperative Adhesion Barriers and Uterine Tissue Engineering (2025) · cites this paper as [199]

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Authors
Pacharra, S., McMahon, S., Duffy, P., Basnett, P., Yu, W., Seisel, S., Stervbo, U., Babel, N., Roy, I., Viebahn, R., Wang, W., & Salber, J.
Affiliations
  • Ruhr University Bochum (DE)
  • University of Westminster (GB)
  • University Hospitals of the Ruhr-University of Bochum (DE)
  • University of Sheffield (GB)
  • Universitätsklinikum Knappschaftskrankenhaus Bochum (DE)
  • University College Dublin (IE)
Corresponding author
Salber, Jochen
ORCID
Venue
Frontiers in Bioengineering and Biotechnology
Publisher
Frontiers Media SA
Published
2020-08-13
Volume
8
Pages
991-991
ISSN
2296-4185 (ISSN-L)
DOI
10.3389/fbioe.2020.00991
PMC
PMC7438451
OpenAlex
W3049743017
Cited by
25
References
2
Citations by year
2026: 8 · 2025: 7 · 2024: 5 · 2023: 3 · 2021: 2
FWCI
0.33
Type
journal article
Language
en
Open access
Licence
Locations
  • Frontiers in Bioengineering and Biotechnology (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • White Rose Research Online (University of Leeds, The University of Sheffield, University of York) (acceptedVersion)
  • WestminsterResearch (University of Westminster) (acceptedVersion, cc-by)
  • Dokumentenrepositorium der RUB (Ruhr University Bochum) (submittedVersion, cc-by)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • Irish Research Council
  • Seventh Framework Programme
Topics
  • Physical Sciences › Materials Science › Biomaterials › biodegradable polymer synthesis and properties
  • Physical Sciences › Materials Science › Surfaces, Coatings and Films › Polymer Surface Interaction Studies
  • Health Sciences › Medicine › Surgery › Coronary Interventions and Diagnostics
Keywords
polyethylene glycol functionalization, cytocompatibility, hemocompatibility, endothelial cell adhesion, platelet adhesion, leukocyte activation, hemolysis, water contact angle, thrombosis

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