The DOI matches its Crossref record; 142 of the 142 references it cites were found in a registry or an index.
Challenges Associated With the Formation of Recombinant Protein Inclusion Bodies in Escherichia coli and Strategies to Address Them for Industrial Applications
Open accessAbstract
Recombinant proteins are becoming increasingly important for industrial applications, where Escherichia coli is the most widely used bacterial host for their production. However, the formation of inclusion bodies is a frequently encountered challenge for producing soluble and functional recombinant proteins. To overcome this hurdle, different strategies have been developed through adjusting growth conditions, engineering host strains of E. coli, altering expression vectors, and modifying the proteins of interest. These approaches will be comprehensively highlighted with some of the new developments in this review. Additionally, the unique features of protein inclusion bodies, the mechanism and influencing factors of their formation, and their potential advantages will also be discussed.
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Metadata
- Authors
- Bhatwa, A., Wang, W., Hassan, Y. I., Abraham, N., Li, X.-Z., & Zhou, T.
- Venue
- Frontiers in Bioengineering and Biotechnology
- Publisher
- Frontiers Media SA
- Published
- 2021-02-10
- Volume
- 9
- Pages
- 630551-630551
- ISSN
- 2296-4185 (ISSN-L)
- OpenAlex
- W3129007842
- Cited by
- 317
- References
- 142
- FWCI
- 10.60
- Type
- journal article
- Language
- en
- Open access
- gold
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- Bhatwa, A., Wang, W., Hassan, Y. I., Abraham, N., Li, X.-Z., & Zhou, T. (2021). Challenges Associated With the Formation of Recombinant Protein Inclusion Bodies in Escherichia coli and Strategies to Address Them for Industrial Applications. Frontiers in Bioengineering and Biotechnology. https://doi.org/10.3389/fbioe.2021.630551
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