Micropatterned Poly(D,L-Lactide-Co-Caprolactone) Conduits With KHI-Peptide and NGF Promote Peripheral Nerve Repair After Severe Traction Injury
Open accessAbstract
Severe traction injuries after stretch to peripheral nerves are common and challenging to repair. The nerve guidance conduits (NGCs) are promising in the regeneration and functional recovery after nerve injuries. To enhance the repair of severe nerve traction injuries, in this study KHIFSDDSSE (KHI) peptides were grafted on a porous and micropatterned poly(D,L-lactide-co-caprolactone) (PLCL) film (MPLCL), which was further loaded with a nerve growth factor (NGF). The adhesion number of Schwann cells (SCs), ratio of length/width (L/W), and percentage of elongated SCs were significantly higher in the MPLCL-peptide group and MPLCL-peptide-NGF group compared with those in the PLCL group in vitro. The electromyography (EMG) and morphological changes of the nerve after severe traction injury were improved significantly in the MPLCL-peptide group and MPLCL-peptide-NGF group compared with those in the PLCL group in vivo. Hence, the NGCs featured with both bioactive factors (KHI peptides and NGF) and physical topography (parallelly linear micropatterns) have synergistic effect on nerve reinnervation after severe traction injuries.
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Adding bioactive ingredients such as NGF to nerve conduit further enhances induction and promotion effect of scaffold on nerve fiber growth.
“On this basis, bioactive ingredients such as NGF can be further added to the nerve conduit, which can further enhance the induction and promotion effect of the scaffold on nerve fiber growth [185].”
From Application of PLCL as a biodegradable polymer in biomedical engineering (2026) · cites this paper as [185]
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- Authors
- Yu, X., Zhang, D., Liu, C., Liu, Z., Li, Y., Zhao, Q., Gao, C., & Wang, Y.
- Affiliations
- Second Affiliated Hospital of Zhejiang University (CN)
- Zhejiang University (CN)
- Corresponding author
- Gao, Changyou
- Wang, Yong
- Venue
- Frontiers in Bioengineering and Biotechnology
- Publisher
- Frontiers Media SA
- Published
- 2021-12-09
- Volume
- 9
- Pages
- 744230-744230
- ISSN
- 2296-4185 (ISSN-L)
- PMC
- PMC8696012
- OpenAlex
- W4200037340
- Cited by
- 8
- References
- 41
- Citations by year
- 2026: 3 · 2025: 2 · 2023: 1 · 2022: 2
- FWCI
- 0.35
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Frontiers in Bioengineering and Biotechnology (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Natural Science Foundation of Zhejiang Province
- Medical and Health Research Project of Zhejiang Province
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Nerve injury and regeneration
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neural Engineering
- Physical Sciences › Materials Science › Biomaterials › Electrospun Nanofibers in Biomedical Applications
- Keywords
- nerve conduits, peripheral nerve repair, nerve traction injury, micropatterned surfaces, nerve growth factor, nerve regeneration, electromyography, porous scaffolds
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