The DOI matches its Crossref record; 44 of the 44 references it cites were found in a registry or an index.
Analysis of Antimicrobial-Triggered Membrane Depolarization Using Voltage Sensitive Dyes
Open accessAbstract
The bacterial cytoplasmic membrane is a major inhibitory target for antimicrobial compounds. Commonly, although not exclusively, these compounds unfold their antimicrobial activity by disrupting the essential barrier function of the cell membrane. As a consequence, membrane permeability assays are central for mode of action studies analysing membrane-targeting antimicrobial compounds. The most frequently used in vivo methods detect changes in membrane permeability by following internalization of normally membrane impermeable and relatively large fluorescent dyes. Unfortunately, these assays are not sensitive to changes in membrane ion permeability which are sufficient to inhibit and kill bacteria by membrane depolarization. In this manuscript, we provide experimental advice how membrane potential, and its changes triggered by membrane-targeting antimicrobials can be accurately assessed in vivo. Optimized protocols are provided for both qualitative and quantitative kinetic measurements of membrane potential. At last, single cell analyses using voltage-sensitive dyes in combination with fluorescence microscopy are introduced and discussed.
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Cites
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The toxic shock syndrome exotoxin structural gene is not detectably transmitted by a prophage
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Self-citationMode-of-Action of Antimicrobial Peptides: Membrane Disruption vs. Intracellular Mechanisms
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Metadata
- Authors
- te Winkel, J. D., Gray, D. A., Seistrup, K. H., Hamoen, L. W., & Strahl, H.
- Venue
- Frontiers in Cell and Developmental Biology
- Publisher
- Frontiers Media SA
- Published
- 2016-04-13
- Volume
- 4
- Pages
- 29-29
- ISSN
- 2296-634X (ISSN-L)
- OpenAlex
- W2341866225
- Cited by
- 326
- References
- 44
- FWCI
- 7.13
- Type
- journal article
- Language
- en
- Open access
- gold
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- te Winkel, J. D., Gray, D. A., Seistrup, K. H., Hamoen, L. W., & Strahl, H. (2016). Analysis of Antimicrobial-Triggered Membrane Depolarization Using Voltage Sensitive Dyes. Frontiers in Cell and Developmental Biology. https://doi.org/10.3389/fcell.2016.00029
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