The DOI matches its Crossref record; 33 of the 33 references it cites were found in a registry or an index.
Small-Molecule Binding Aptamers: Selection Strategies, Characterization, and Applications
Open accessAbstract
Aptamers are single-stranded, synthetic oligonucleotides that fold into 3-dimensional shapes capable of binding non-covalently with high affinity and specificity to a target molecule. They are generated via an in vitro process known as the Systematic Evolution of Ligands by EXponential enrichment, from which candidates are screened and characterized, and then used in various applications. These applications range from therapeutic uses to biosensors for target detection. Aptamers for small molecule targets such as toxins, antibiotics, molecular markers, drugs, and heavy metals will be the focus of this review. Their accurate detection is needed for the protection and wellbeing of humans and animals. However, the small molecular weights of these targets, including the drastic size difference between the target and the oligonucleotides, make it challenging to select, characterize, and apply aptamers for their detection. Thus, recent (since 2012) notable advances in small molecule aptamers, which have overcome some of these challenges, are presented here, while defining challenges that still exist are discussed.
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Cites
Tuerk, C., & Gold, L. (1990) · Science · doi:10.1126/science.2200121 · Cited by 10,405
In vitro selection of RNA molecules that bind specific ligands
Ellington, A. D., & Szostak, J. W. (1990) · Nature · doi:10.1038/346818a0 · Cited by 9,812
Adaptive Recognition by Nucleic Acid Aptamers
Hermann, T., & Patel, D. J. (2000) · Science · doi:10.1126/science.287.5454.820 · Cited by 1,650
Structure-Switching Signaling Aptamers
Nutiu, R., & Li, Y. (2003) · Journal of the American Chemical Society · doi:10.1021/ja028962o · Cited by 1,040
Filonov, G. S., Moon, J. D., Svensen, N., & Jaffrey, S. R. (2014) · Journal of the American Chemical Society · doi:10.1021/ja508478x · Cited by 780
The Chemical Biology of Aptamers
Mayer, G. (2009) · Angewandte Chemie International Edition · doi:10.1002/anie.200804643 · Cited by 592
Rohloff, J. C., Gelinas, A. D., Jarvis, T. C., Ochsner, U. A., Schneider, D. J., Gold, L., & Janjic, N. (2014) · Molecular Therapy - Nucleic Acids · doi:10.1038/mtna.2014.49 · Cited by 533
Challenges and Opportunities for Small Molecule Aptamer Development
McKeague, M., & DeRosa, M. C. (2012) · Journal of Nucleic Acids · doi:10.1155/2012/748913 · Cited by 466
Self-citationFluMag-SELEX as an advantageous method for DNA aptamer selection
Stoltenburg, R., Reinemann, C., & Strehlitz, B. (2005) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-005-3388-9 · Cited by 367
Aptamer-Conjugated Nanoparticles for the Collection and Detection of Multiple Cancer Cells
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Cited by
SELEX: Critical factors and optimization strategies for successful aptamer selection
Kohlberger, M., & Gadermaier, G. (2022) · Biotechnology and Applied Biochemistry · doi:10.1002/bab.2244 · Cited by 341
Development of an inducible anti-VEGF rAAV gene therapy strategy for the treatment of wet AMD
Reid, C. A., Nettesheim, E. R., Connor, T. B., & Lipinski, D. M. (2018) · Scientific Reports · doi:10.1038/s41598-018-29726-7 · Cited by 65
Klinghammer, S., Voitsekhivska, T., Licciardello, N., Kim, K., Baek, C.-K., Cho, H., Wolter, K.-J., Kirschbaum, C., Baraban, L., & Cuniberti, G. (2020) · ACS Sensors · doi:10.1021/acssensors.0c02267 · Cited by 62
Lipinski, D. M. (2019) · Advances in Experimental Medicine and Biology · doi:10.1007/978-3-030-27378-1_13 · Cited by 8
Metadata
- Authors
- Ruscito, A., & DeRosa, M. C.
- Venue
- Frontiers in Chemistry
- Publisher
- Frontiers Media SA
- Published
- 2016-05-10
- Volume
- 4
- Pages
- 14-14
- ISSN
- 2296-2646 (ISSN-L)
- OpenAlex
- W2361791156
- Cited by
- 391
- References
- 33
- FWCI
- 13.99
- Type
- journal article
- Language
- en
- Open access
- gold
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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