Cited by 62Advanced biosensing and bioanalysis techniquesNanowire Synthesis and ApplicationsAnalytical Chemistry and Sensors

Nanosensor-Based Real-Time Monitoring of Stress Biomarkers in Human Saliva Using a Portable Measurement System

Klinghammer, Stephanie, Voitsekhivska, Tetiana, Licciardello, Nadia, Kim, Kihyun, Baek, Chang-Ki, Cho, Hyeonsu, Wolter, Klaus-Jürgen, Kirschbaum, Clemens, Baraban, Larysa, Cuniberti, Gianaurelio·2020·ACS Sensors

Abstract

Small molecules with no or little charge are considered to have minimal impact on signals measured by field effect transistor (FET) sensors. This fact typically excludes steroids from the family of analytes, detected by FETs. We present a portable multiplexed platform based on an array of nanowire sensors for label-free monitoring of daytime levels of the stress hormone cortisol in saliva samples, obtained from multiple donors. To achieve an effective quantification of the cortisol with FETs, we rely on the specific DNA aptamer sequences as receptors, bringing the complex "target-receptor" closer to the nanowire surface. Upon binding, cortisol induces conformational changes of negatively charged aptamers, wrapping it into a close proximity to the silicon nanowires, to efficiently modulate their surface potential. Thus, the sensors allow for a real-time assessment of the steroid biomarkers at low nanomolar concentration. The measurement platform is designed in a building-block concept, consisting of a modular measuring unit and a customizable biochip board, and operates using a complementary metal-oxide-semiconductor-integrated multiplexer. The platform is capable of continuous and simultaneous measurement of samples from multiple patients. Cortisol levels detected with the presented platform agreed well with the results obtained with a commercial high-sensitivity immunoassay.

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Crossref · 10.1021/acssensors.0c02267

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Authors
Klinghammer, S., Voitsekhivska, T., Licciardello, N., Kim, K., Baek, C.-K., Cho, H., Wolter, K.-J., Kirschbaum, C., Baraban, L., & Cuniberti, G.
Affiliations
  • Max Bergmann Center of Biomaterials (DE)
  • Helmholtz-Zentrum Dresden-Rossendorf (DE)
  • Pohang University of Science and Technology (KR)
  • Technische Universität Dresden (DE)
Corresponding author
Baraban, Larysa
Venue
ACS Sensors
Published
2020-12-03
Volume
5
Issue
12
Pages
4081-4091
ISSN
2379-3694 (ISSN-L)
OpenAlex
W3110229389
Cited by
62
References
64
Citations by year
2026: 9 · 2025: 16 · 2024: 12 · 2023: 12 · 2022: 10 · 2021: 3
FWCI
2.03
Type
journal article
Language
en
Open access
closed
Locations
  • ACS Sensors (publishedVersion)
  • PubMed (publishedVersion)
  • Open Access System for Information Sharing (Pohang University of Science and Technology) (submittedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced biosensing and bioanalysis techniques
  • Physical Sciences › Engineering › Biomedical Engineering › Nanowire Synthesis and Applications
  • Physical Sciences › Chemical Engineering › Bioengineering › Analytical Chemistry and Sensors
Keywords
cortisol, stress biomarkers, saliva, field-effect transistor sensors, nanowire sensors, DNA aptamers, label-free detection, real-time monitoring, steroid hormones, biochips, conformational change
MeSH terms
Humans, Saliva, Transistors, Electronic, Biosensing Techniques, Biomarkers, Nanowires

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