Enhanced healing and antimicrobial efficacy of Chitosan-g-Polyacrylamide in a rat model of gingival ulcers
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Patients in dental hospitals often experience oral ulcerative lesions, which lead to pain and affect the patient’s quality of life. At present, the goal of treating oral ulcerative lesions with drugs is to reduce inflammation and promote ulcer healing. However, very few antibacterial and hemostatic drugs are designed to be suitable for the microenvironment of gingival ulcers. Based on this, we have designed a natural therapeutic agent for oral ulcerative lesions that meets the various requirements of oral ulcerative lesion medication. The chitosan-g-polyacrylamide (CP) copolymer is composed of chitosan as the main chain and polyacrylamide polymers as the side chains. Antibacterial experiments show that this polymer can effectively inhibit the proliferation of Gram-negative (Escherichia coli) and Gram-positive bacteria (Staphylococcus aureus). In vitro cell experiments also show that the CP copolymer is non-toxic, which is conducive to ulcer wound healing. Coagulation experiments prove that the CP copolymer can accelerate blood coagulation to stop bleeding. In experiments using a Wistar rat gingival ulcer model, the CP copolymer significantly promoted ulcer healing and shorten the healing time. These results indicate that the CP copolymer may serve as a potential therapeutic agent for oral ulcerative lesions.
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Polymer nanomicelles could be easily functionally optimized due to their flexible polymer chains; physical properties can be tailored using various hydrophilic or hydrophobic fragments, such as PEG-based micelles for ameliorative dispersity and chitosan micelles for enhanced uptake.
“Moreover, polymer nanomicelles could be easily functionally optimized due to their flexible polymer chains. The physical properties of them could be tailored using various hydrophilic or hydrophobic fragments, such as PEG-based micelles for ameliorative dispersity and chitosan (CS) micelles for enhanced uptake [207].”
From From design to Deployment: The innovative role of nanomicelles in targeted ophthalmic therapeutics (2025) · cites this paper as [207]
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1 retracted
Oral ulcer healing and anti-Candida efficacy of an alcohol-free chitosan-curcumin mouthwash.
Mahattanadul, S., Mustafa, M. W., Kuadkaew, S., Pattharachayakul, S., Ungphaiboon, S., & Sawanyawisuth, K. (2018) · PubMed · doi:10.26355/eurrev_201810_16173 · Cited by 17 · see citing works
RetractedBerger, J., Reist, M., Mayer, J. M., Felt, O., Peppas, N. A., & Gurny, R. (2003) · European Journal of Pharmaceutics and Biopharmaceutics · doi:10.1016/s0939-6411(03)00161-9 · Cited by 2,166 · see citing works
Perspectives on cancer therapy-induced mucosal injury
Sonis, S. T., Elting, L. S., Keefe, D. M. K., Peterson, D. E., Schubert, M., Hauer‐Jensen, M., Bekele, B. N., Raber‐Durlacher, J. E., Donnelly, J. P., & Rubenstein, E. B. (2004) · Cancer · doi:10.1002/cncr.20162 · Cited by 1,420 · see citing works
Antibacterial action of chitosan and carboxymethylated chitosan
Liu, X., Guan, Y. L., Yang, D. Z., Li, Z., & Yao, K. D. (2000) · Journal of Applied Polymer Science · doi:10.1002/1097-4628(20010214)79:7 · Cited by 1,177 · see citing works
Insights into the mode of action of chitosan as an antibacterial compound.
Raafat, D., von Bargen, K., Haas, A., & Sahl, H.-G. (2008) · Applied and Environmental Microbiology · doi:10.1128/aem.00453-08 · Cited by 888 · see citing works
Pathogens and host immunity in the ancient human oral cavity
Warinner, C., Rodrigues, J. F. M., Vyas, R., Trachsel, C., Shved, N., Grossmann, J., Radini, A., Hancock, Y., Tito, R. Y., Fiddyment, S., et al. (2014) · Nature Genetics · doi:10.1038/ng.2906 · Cited by 618 · see citing works
Antibacterial activity of shrimp chitosan against Escherichia coli.
Tsai, G., & Su, W.-H. (1999) · Journal of Food Protection · doi:10.4315/0362-028x-62.3.239 · Cited by 593 · see citing works
Lu, Z., Gao, J., He, Q., Wu, J., Liang, D., Yang, H., & Chen, R. (2016) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2016.09.051 · Cited by 379 · see citing works
Potential Use of Chitosan as a Cell Scaffold Material for Cartilage Tissue Engineering
Nettles, D. L., Elder, S. H., & Gilbert, J. A. (2002) · Tissue Engineering · doi:10.1089/107632702320934100 · Cited by 345 · see citing works
Effect of abiotic factors on the antibacterial activity of chitosan against waterborne pathogens
CHUNG, Y. (2003) · Bioresource Technology · doi:10.1016/s0960-8524(03)00002-6 · Cited by 302 · see citing works
Vieira, E. L. M., Leonel, A. J., Sad, A. P., Beltrão, N. R. M., Costa, T. F., Ferreira, T. M. R., Gomes-Santos, A. C., Faria, A. M. C., Peluzio, M. C. G., Cara, D. C., et al. (2012) · The Journal of Nutritional Biochemistry · doi:10.1016/j.jnutbio.2011.01.007 · Cited by 290 · see citing works
Silver and nanoparticles of silver in wound dressings: a review of efficacy and safety
Wilkinson, L., White, R. J., & Chipman, J. K. (2011) · Journal of Wound Care · doi:10.12968/jowc.2011.20.11.543 · Cited by 281 · see citing works
Physical, Mechanical, and Antibacterial Properties of Chitosan/PEO Blend Films
Zivanovic, S., Li, J., Davidson, P. M., & Kit, K. (2007) · Biomacromolecules · doi:10.1021/bm061140p · Cited by 277 · see citing works
Systematic review of basic oral care for the management of oral mucositis in cancer patients
McGuire, D. B., Fulton, J. S., Park, J., Brown, C. G., Corrêa, M. E. P., Eilers, J., Elad, S., Gibson, F., Oberle-Edwards, L., Bowen, J. M., et al. (2013) · Supportive Care in Cancer · doi:10.1007/s00520-013-1942-0 · Cited by 258 · see citing works
Polyacrylamide Hydrogels for Cell Mechanics: Steps Toward Optimization and Alternative Uses
Kandow, C. E., Georges, P. C., Janmey, P. A., & Beningo, K. A. (2007) · Methods in cell biology · doi:10.1016/s0091-679x(07)83002-0 · Cited by 241 · see citing works
Effect of chitosan molecular weight and deacetylation degree on hemostasis
Yang, J. H., Tian, F., Wang, Z., Wang, Q., Zeng, Y., & Chen, S. (2007) · Journal of Biomedical Materials Research Part B Applied Biomaterials · doi:10.1002/jbm.b.30853 · Cited by 235 · see citing works
Risbud, R. R. B. M. V. (2000) · Drug Delivery · doi:10.1080/107175400266623 · Cited by 203 · see citing works
Surface active properties of chitosan and its derivatives
Elsabee, M. Z., Morsi, R. E., & Al-Sabagh, A. M. (2009) · Colloids and Surfaces B Biointerfaces · doi:10.1016/j.colsurfb.2009.06.021 · Cited by 173 · see citing works
Hydroxyapatite–chitin materials as potential tissue engineered bone substitutes
Ge, Z., Baguenard, S., Lim, L. Y., Wee, A., & Khor, E. (2003) · Biomaterials · doi:10.1016/s0142-9612(03)00612-4 · Cited by 165 · see citing works
Production of ultra-fine bioresorbable carbonated hydroxyapatite
Ramalingam, M., & Ramakrishna, S. (2005) · Acta Biomaterialia · doi:10.1016/j.actbio.2005.09.005 · Cited by 136 · see citing works
Studies on the microspheres comprised of reconstituted collagen and hydroxyapatite
Wu, T.-J., Huang, H.-H., Lan, C., Lin, C.-H., Hsu, F., & Wang, Y.-J. (2003) · Biomaterials · doi:10.1016/s0142-9612(03)00576-3 · Cited by 133 · see citing works
Lewandowska, K. (2006) · Journal of Applied Polymer Science · doi:10.1002/app.25247 · Cited by 128 · see citing works
Inverted‐Colloidal‐Crystal Hydrogel Matrices as Three‐Dimensional Cell Scaffolds
Zhang, Y., Wang, S., Eghtedari, M., Motamedi, M., & Kotov, N. A. (2005) · Advanced Functional Materials · doi:10.1002/adfm.200400325 · Cited by 123 · see citing works
Raber‐Durlacher, J. E., Bültzingslöwen, I. V., Logan, R. M., Bowen, J. M., Al-Azri, A. R., Everaus, H., Gerber, E., Gómez, J. G., Pettersson, B., Soga, Y., et al. (2012) · Supportive Care in Cancer · doi:10.1007/s00520-012-1594-5 · Cited by 122 · see citing works
IL-17-mediated antifungal defense in the oral mucosa is independent of neutrophils
Trautwein-Weidner, K., Gladiator, A., Nur, S., Diethelm, P., & Leibundgut-Landmann, S. (2014) · Mucosal Immunology · doi:10.1038/mi.2014.57 · Cited by 116 · see citing works
Emerging Supramolecular Therapeutic Carriers Based on Host–Guest Interactions
Karim, A. A., Dou, Q., Li, Z., & Loh, X. J. (2016) · Chemistry - An Asian Journal · doi:10.1002/asia.201501434 · Cited by 107 · see citing works
Chitosan and its salts for mucosal and transmucosal delivery
Bonferoni, M. C., Sandri, G., Rossi, S., Ferrari, F., & Caramella, C. M. (2009) · Expert Opinion on Drug Delivery · doi:10.1517/17425240903114142 · Cited by 93 · see citing works
Development of alginate microspheres as nystatin carriers for oral mucosa drug delivery
Martín, M. J., Calpena, A. C., Fernández-Campos, F., Mallandrich, M., Gálvez, P., & Clares, B. (2014) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2014.09.032 · Cited by 88 · see citing works
Distinct phenotype and therapeutic potential of gingival fibroblasts
Häkkinen, L., Larjava, H., & Fournier, B. (2014) · Cytotherapy · doi:10.1016/j.jcyt.2014.04.004 · Cited by 85 · see citing works
Ren, D., Zheng, S., Wu, F., Yang, W. M., Liu, Z., & Yang, M. (2013) · Journal of Applied Polymer Science · doi:10.1002/app.39953 · Cited by 79 · see citing works
Wang, J., & Wang, H. (2011) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2011.01.016 · Cited by 77 · see citing works
Self-citationAutonomous Chitosan-Based Self-Healing Hydrogel Formed through Noncovalent Interactions
Zhang, Z., Liow, S. S., Xue, K., Zhang, X., Li, Z., & Loh, X. J. (2019) · ACS Applied Polymer Materials · doi:10.1021/acsapm.9b00317 · Cited by 57 · see citing works
One-pot synthesis of dual-stimuli responsive hybrid PNIPAAm-chitosan microgels
Echeverría, C., Soares, P. I. P., Robalo, A., Pereira, L. C. J., Novo, C., Ferreira, I., & Borges, J. P. (2015) · Materials & Design · doi:10.1016/j.matdes.2015.07.170 · Cited by 45 · see citing works
Wang, W., & Yu, W. (2015) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2015.03.040 · Cited by 45 · see citing works
Vahabi, S., Vaziri, S., Torshabi, M., & Esfahrood, Z. R. (2015) · PubMed Central · Cited by 42 · see citing works
Shao, Y., & Zhou, H. (2019) · Journal of Dermatological Treatment · doi:10.1080/09546634.2019.1610548 · Cited by 41 · see citing works
Arrays of polyacrylamide hydrogels using a carbodiimide‐mediated crosslinking reaction
Sheng, Q., Tian, W., Lapierre, F., Gao, S., Mulder, R. J., Zhu, Y., Kozielski, K. A., & Wood, C. D. (2014) · Journal of Applied Polymer Science · doi:10.1002/app.40416 · Cited by 38 · see citing works
Treatment of chemically induced oral ulcer using adipose‐derived mesenchymal stem cell sheet
Lee, D. Y., Kim, H., Shim, I. K., Kanai, N., Okano, T., & Kwon, S. K. (2016) · Journal of Oral Pathology and Medicine · doi:10.1111/jop.12517 · Cited by 35 · see citing works
Chemotherapy- and radiotherapy-induced oral mucositis: pathobiology, epidemiology and management.
Feller, L., Essop, R., Wood, N. H., Khammissa, R. A. G., Chikte, U. M. E., Meyerov, R., & Lemmer, J. (2010) · PubMed · Cited by 32 · see citing works
Huang, C.-H., Wang, C.-F., Don, T., & Chiu, W. (2013) · Cellulose · doi:10.1007/s10570-013-9951-1 · Cited by 32 · see citing works
Miscibility and physical properties of chitosan and polyacrylamide blends
Lewandowska, K. (2015) · Journal of Molecular Liquids · doi:10.1016/j.molliq.2015.05.049 · Cited by 25 · see citing works
Aravind, K., Padmavathi, R., & Dharmalingam, S. (2012) · Carbohydrate Research · doi:10.1016/j.carres.2011.12.027 · Cited by 18 · see citing works
Bone Marrow–derived Cell Therapy for Oral Mucosal Repair after Irradiation
Tariq Mahmood, T. I., Sumita, Y., Minamizato, T., Umebayashi, M., Liu, Y., Tran, S. D., & Asahina, I. (2014) · Journal of Dental Research · doi:10.1177/0022034514541124 · Cited by 18 · see citing works
Lee, S., Jung, I., Yu, S., & Hong, J. P. (2014) · Archives of Plastic Surgery · doi:10.5999/aps.2014.41.5.466 · Cited by 7 · see citing works
Li, J., Wei, C. P., Wang, F. M., Dong, L. D., Sun, S. B., & Liu, Q. Y. (2016) · Materials science forum · doi:10.4028/www.scientific.net/msf.852.1277 · Cited by 1 · see citing works
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Nunes, Y. L., de Menezes, F. L., de Sousa, I. G., Cavalcante, A. L. G., Cavalcante, F. T. T., Moreira, K. D. S., de Oliveira, A. L. B., Mota, G. F., Souza, J. E. D. S., Falcão, Í. R. D. A., et al. (2021) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2021.04.004 · Cited by 139 · see citing works
Li, Z., Liu, M., Ke, L., Wang, L.-J., Wu, C., Li, C., Li, Z., & Wu, Y.-L. (2021) · Nanoscale Advances · doi:10.1039/d1na00596k · Cited by 59 · see citing works
Self-citationPolymeric Micelles as Drug Delivery System: Recent Advances, Approaches, Applications and Patents
Jain, A., Bhardwaj, K., & Bansal, M. (2023) · Current Drug Safety · doi:10.2174/1574886318666230605120433 · Cited by 33 · see citing works
He, S., Bai, J., Liu, Y., Zeng, Y., Wang, L., Chen, X., Wang, J., Weng, J., Zhao, Y., Peng, W., et al. (2023) · International Journal of Pharmaceutics · doi:10.1016/j.ijpharm.2023.123607 · Cited by 29 · see citing works
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Chang, Y., Zhao, W. H., Li, W., Zhang, Q., & Wang, G. (2024) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2024.133858 · Cited by 20 · see citing works
Mohammed, E., Aboulkhair, A. G., & Tawifq, M. M. (2021) · Clinical Oral Investigations · doi:10.1007/s00784-021-04293-w · Cited by 16 · see citing works
Advances in smart stimuli-responsive materials for oral wound healing
Liu, X., Huang, X., Liu, N., Zhu, L., Zhao, S., Tang, S., & Wang, P. (2025) · Frontiers in Chemistry · doi:10.3389/fchem.2025.1725373 · Cited by 11 · see citing works
Hong, W., Zhang, Q., Lin, L., Zhang, H., Lei, X., Wang, Y., Zhang, D., Jia, Z., Wang, L., Pang, J., et al. (2025) · Advanced Functional Materials · doi:10.1002/adfm.202525950 · Cited by 9 · see citing works
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Onugwu, A. L., Emencheta, S. C., Sabastine, R. N., Onugwu, S. O., Echezona, A. C., Nwagwu, C. S., Chime, S. A., Ezike, T. C., Eze, O. P., Onyeyilim, E. L., et al. (2024) · Journal of Drug Delivery Science and Technology · doi:10.1016/j.jddst.2024.106219 · Cited by 9 · see citing works
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Sun, M., Wang, J., Fan, W., Wang, K., Wang, Y., Zhang, J., Ding, S., Zhang, C., Feng, Z., Wang, Y.-G., et al. (2025) · Materials today. Bio · doi:10.1016/j.mtbio.2025.102537 · Cited by 7 · see citing works
“The physical properties of them could be tailored using various hydrophilic or hydrophobic fragments, such as PEG-based micelles for ameliorative dispersity and chitosan (CS) micelles for enhanced uptake [207].”
Taha, R. M., & Said, R. H. M. (2023) · Egyptian Dental Journal /Egyptian Dental Journal · doi:10.21608/edj.2022.130994.2055 · Cited by 2 · see citing works
- Authors
- Zheng, Y., Ke, L., Lu, Y., Zuo, Q., Deng, G., Wang, H., & Zeng, X.
- Affiliations
- Xiamen Medical College (CN)
- Xiamen University (CN)
- Corresponding author
- Zheng, Yanfen
- Venue
- Frontiers in Chemistry
- Publisher
- Frontiers Media SA
- Published
- 2020-04-24
- Volume
- 8
- Pages
- 273-273
- ISSN
- 2296-2646 (ISSN-L)
- PMC
- PMC7193946
- OpenAlex
- W3020230379
- Cited by
- 11
- References
- 45
- Citations by year
- 2025: 3 · 2024: 2 · 2023: 3 · 2021: 3
- FWCI
- 0.83
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Frontiers in Chemistry (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Xiamen Municipal Bureau of Science and Technology (3502Z20174025)
- Topics
- Health Sciences › Medicine › Rehabilitation › Wound Healing and Treatments
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmaceutical Science › Advanced Drug Delivery Systems
- Health Sciences › Medicine › Hematology › Hemostasis and retained surgical items
- Keywords
- gingival ulcer, wound healing, antimicrobial activity, antibacterial polymers, hemostasis, coagulation, Escherichia coli, Staphylococcus aureus
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