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Vibration therapy as an effective approach to improve bone healing in diabetic rats
Open accessAbstract
Objective To investigate the effects of vibration therapy on fracture healing in diabetic and non-diabetic rats. Methods 148 rats underwent fracture surgery and were assigned to four groups: (1) SHAM: weight-matched non-diabetic rats, (2) SHAM+VT: non-diabetic rats treated with vibration therapy (VT), (3) DM: diabetic rats, and (4) DM+VT: diabetic rats treated with VT. Thirty days after diabetes induction with streptozotocin, animals underwent bone fracture, followed by surgical stabilization. Three days after bone fracture, rats began VT. Bone healing was assessed on days 14 and 28 post-fracture by serum bone marker analysis, and femurs collected for dual-energy X-ray absorptiometry, micro-computed tomography, histology, and gene expression. Results Our results are based on 88 animals. Diabetes led to a dramatic impairment of bone healing as demonstrated by a 17% reduction in bone mineral density and decreases in formation-related microstructural parameters compared to non-diabetic control rats (81% reduction in bone callus volume, 69% reduction in woven bone fraction, 39% reduction in trabecular thickness, and 45% in trabecular number). These changes were accompanied by a significant decrease in the expression of osteoblast-related genes (Runx2, Col1a1, Osx), as well as a 92% reduction in serum insulin-like growth factor I (IGF-1) levels. On the other hand, resorption-related parameters were increased in diabetic rats, including a 20% increase in the callus porosity, a 33% increase in trabecular separation, and a 318% increase in serum C terminal telopeptide of type 1 collagen levels. VT augmented osteogenic and chondrogenic cell proliferation at the fracture callus in diabetic rats; increased circulating IGF-1 by 668%, callus volume by 52%, callus bone mineral content by 90%, and callus area by 72%; and was associated with a 19% reduction in circulating receptor activator of nuclear factor kappa beta ligand (RANK-L). Conclusions Diabetes had detrimental effects on bone healing. Vibration therapy was effective at counteracting the significant disruption in bone repair induced by diabetes, but did not improve fracture healing in non-diabetic control rats. The mechanical stimulus not only improved bone callus quality and quantity, but also partially restored the serum levels of IGF-1 and RANK-L, inducing bone formation and mineralization, thus creating conditions for adequate fracture repair in diabetic rats.
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Vibration exposure normalized fracture healing in diabetic and ovariectomized rats with osteoporosis but did not affect bone healing in healthy rats.
“While vibration exposure was shown to normalize fracture healing in diabetic (37) and ovariectomized rats with osteoporosis (38), it did not affect bone healing in healthy rats (37).”
From Methods to accelerate fracture healing – a narrative review from a clinical perspective (2024) · cites this paper as [37]
The Abstract’s Conclusions report that vibration counteracted disrupted fracture repair in diabetic rats and did not improve healing in non-diabetic controls. They do not establish that diabetic healing was fully normalized; the Discussion also reports impaired callus microstructure in healthy sham rats at day 14, so “did not affect” is too absolute. The ovariectomized-rat finding is attached to a different citation in the citing sentence. (LLM, conf=0.87)
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“While vibration exposure was shown to normalize fracture healing in diabetic (37) and ovariectomized rats with osteoporosis (38), it did not affect bone healing in healthy rats (37).”
BackgroundGoncharov, E. N., Koval, O. A., Bezuglov, E., Engelgard, M., Igorevich, E. I., Kotenko, K. V., Ramírez, M. D. J. E., & Montemurro, N. (2024) · Biomedicines · doi:10.3390/biomedicines12020342 · Cited by 14
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Metadata
- Authors
- Campos, M. S., Volpon, J. B., Ximenez, J. P. B., Franttini, A. P., Dalloul, C. E., Sousa-Neto, M. D., Silva, R. A., Kacena, M. A., & Zamarioli, A.
- Affiliations
- Universidade de São Paulo (BR)
- Indiana University School of Medicine (US)
- Richard L. Roudebush VA Medical Center (US)
- Corresponding author
- Zamarioli, Ariane
- Venue
- Frontiers in Endocrinology
- Publisher
- Frontiers Media SA
- Published
- 2022-08-19
- Volume
- 13
- Pages
- 909317-909317
- ISSN
- 1664-2392 (ISSN-L)
- PMC
- PMC9437439
- OpenAlex
- W4292373070
- Cited by
- 9
- References
- 40
- Citations by year
- 2026: 2 · 2025: 1 · 2024: 3 · 2023: 3
- FWCI
- 1.81
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Frontiers in Endocrinology (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- IUScholarWorks (Indiana University) (submittedVersion, cc-by)
- Funders
- Fundação de Amparo à Pesquisa do Estado de São Paulo
- Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
- National Institutes of Health
- Topics
- Health Sciences › Medicine › Orthopedics and Sports Medicine › Effects of Vibration on Health
- Health Sciences › Medicine › Orthopedics and Sports Medicine › Bone fractures and treatments
- Health Sciences › Medicine › Orthopedics and Sports Medicine › Bone health and osteoporosis research
- Keywords
- vibration therapy, fracture healing, diabetic bone healing, diabetes mellitus, osteogenic differentiation, chondrogenic differentiation, insulin-like growth factor I, RANKL, bone mineral density, bone callus, osteoclastogenesis, mechanical stimulation, streptozotocin-induced diabetes, osteoblast gene expression, trabecular bone microarchitecture, bone resorption
- MeSH terms
- Animals, Bony Callus, Diabetes Mellitus, Insulin-Like Growth Factor I, Vibration, Fracture Healing, Fractures, Bone, Rats, X-Ray Microtomography
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